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公开(公告)号：US11591561B2
公开(公告)日：2023-02-28
申请号：US16168464
申请日：2018-10-23
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Charles A. Lissandrello , Ryan A. Dubay , Rebecca Christianson , Jenna Leigh Balestrini , Peter Hsi , Jason Fiering
Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.
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公开(公告)号：US11584908B2
公开(公告)日：2023-02-21
申请号：US16137478
申请日：2018-09-20
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Kenneth T. Kotz , Bryan D. Teece , James Gillett Truslow , Nathan Francis Moore , Jeffrey T. Borenstein , Vishal Tandon
IPC: C12M1/02 , C12M1/12 , C12N15/87 , C12M1/00 , C12N15/86 , C12M3/06 , C12M1/40 , C12M1/34 , A61K48/00
Abstract: A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
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公开(公告)号：US11439749B2
公开(公告)日：2022-09-13
申请号：US16169498
申请日：2018-10-24
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Marcello Peppi , Vishal Tandon
Abstract: The present solution provides trans-round window membrane (RWM) drug delivery. As an overview, the system can include a micropump that is connected to a flexible cannula. The cannula can include a stiffened and sharpened tip to facilitate insertion through the RWM. The cannula can be inserted through the RWM to improve the distribution of the delivered drug throughout the inner ear. The present solution can function as a small implantable or wearable device that can be used for both chronic and acute trans-RWM drug delivery. With this configuration, the micropump can constantly or intermittently deliver, over a period of days to months, small volumes of drugs from an internal reservoir.
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公开(公告)号：US11015161B2
公开(公告)日：2021-05-25
申请号：US16123539
申请日：2018-09-06
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Jeffrey T. Borenstein , Jenna L. Balestrini , Vishal Tandon , Louis B. Kratchman
Abstract: Microfluidic devices and associated methods are disclosed. A microfluidic device includes a target entrainment channel and an effluent channel on opposing sides of a semipermeable membrane. A restrictor channel that is narrower than the effluent channel is interposed between the semipermeable membrane and the effluent channel. Fluid that flows from the target entrainment channel, through the semipermeable membrane and the restrictor channel to the effluent channel, pins target cells along the center of the target entrainment channel for electroporation using an electrode in the channel.
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公开(公告)号：US10596267B2
公开(公告)日：2020-03-24
申请号：US15631933
申请日：2017-06-23
Applicant: The Charles Stark Draper Laboratory, Inc. , Massachusetts Eye and Ear Infirmary , Massachusetts Institute of Technology
Inventor： Jeffrey T. Borenstein , Erin Pararas , Ernest S. Kim , Vishal Tandon , Andrew Ayoob , Michael McKenna , William Sewell , Marcello Peppi , Marc Weinberg , Robert Langer
IPC: A61K9/00 , A61K9/06 , A61K47/10 , A61K47/60 , A61K31/498
Abstract: The present disclosure provides compositions and methods for treating an auditory disease in a subject in need thereof comprising administering an effective amount of a gel-based precursor that includes an inner ear-specific therapeutic compound directly into the cochlea of the subject.
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Patent Agency Ranking
公开(公告)号：US20190292510A1
公开(公告)日：2019-09-26
申请号：US16359629
申请日：2019-03-20
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Jonathan R. Coppeta , Kenneth Kotz , Heena K. Mutha , Jenna Leigh Balestrini
Abstract: A viral transduction and/or electroporation device has s a membrane separating two chambers and two electroporation electrodes for the chambers. An electrical voltage source is used for establishing an electrical field across the membrane and between the two electrodes. In operation, fluid is flowed into the chambers including fluid containing electroporation cargo and viral transduction solution and an electrical field is established across the membrane and between the electrodes to electroporate cells pinned to the membrane and transfecting the cells.
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公开(公告)号：US20240084236A1
公开(公告)日：2024-03-14
申请号：US18515493
申请日：2023-11-21
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Charles A. Lissandrello , Jenna L. Balestrini , Jonathan R. Coppeta , Patricia A. Swierk
CPC classification number: C12M23/16 , C12M35/02 , B01L3/502715
Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
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公开(公告)号：US20200071727A1
公开(公告)日：2020-03-05
申请号：US16557820
申请日：2019-08-30
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Charles A. Lissandrello , Jenna L. Balestrini , Jonathan R. Coppeta , Patricia A. Swierk
Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
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19.发明申请公开(公告)号：US20190010516A1
公开(公告)日：2019-01-10
申请号：US16026709
申请日：2018-07-03
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Daniel K. Freeman , Jonathan R. Coppeta , Jeffrey T. Borenstein , Jenna L. Balestrini
IPC: C12N15/87
Abstract: A method and system of delivering a charged cargo, such as a biomolecule, to a target structure, such as cells, exosomes, other vesicles or micelles, using an electroactive porous membrane. This method comprises contacting an electroactive porous membrane with a fluid flow toward the membrane. The fluid contains charged biomolecules and the membrane and biomolecules are oppositely charged so that the biomolecules in the fluid are trapped on the membrane as the fluid flows through the pores of the membrane. Acceptor cells of interest are pinned to the membrane by the flow of the fluid, thereby aggregating the cells onto the membrane in close proximity to the trapped biomolecules. Finally, the acceptor cells are permeabilized.
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公开(公告)号：US20180179485A1
公开(公告)日：2018-06-28
申请号：US15851393
申请日：2017-12-21
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Jeffrey T. Borenstein , Jenna L. Balestrini , Vishal Tandon , Jonathan R. Coppeta
CPC classification number: C12M35/02 , B01L3/502715 , B01L2200/0647 , C12M23/16 , C12M25/02 , C12M29/00 , C12M41/12 , C12N13/00 , C12N15/87
Abstract: A system and method of using a microfluidic electroporation device for cell treatment is provided. The cell or exosome treatment system can include a microfluidic electroporation device, a voltage source coupled to a plurality of electrodes and a controller coupled to the voltage source. The microfluidic electroporation device can include a fluid receptacle, a semipermeable membrane, and a base including a channel in fluid communication with the fluid receptacle and the semipermeable membrane. A first electrode can be positioned within the fluid receptacle and a second electrode coupled to the base. The second electrode is positioned relative to the first electrode to create an electric field sufficient to electroporate cells or exosomes disposed in the fluid receptacle. The controller can be configured to cause the first and second electrodes to apply voltage electroporating the cells and exosomes.
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