Hepatocellular carcinoma protein marker
    13.
    发明授权
    Hepatocellular carcinoma protein marker 失效
    肝细胞癌蛋白标记

    公开(公告)号:US08207301B2

    公开(公告)日:2012-06-26

    申请号:US12452216

    申请日:2008-06-16

    IPC分类号: C07K14/435

    CPC分类号: C07K14/4748 G01N33/57438

    摘要: Provided are: a method of assessing hepatocellular carcinoma by using a protein with a different phosphorylated state in hepatocellular carcinoma cells compared with non-hepatocellular carcinoma cells; and a hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma formed of the protein. The hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma includes tumor rejection antigen gp96 formed of the amino acid represented by SEQ ID NO: 1, and is measured for its phosphorylated state to detect the presence or absence of hepatocellular carcinoma.

    摘要翻译: 提供:与非肝细胞癌细胞相比,通过使用肝细胞癌细胞中具有不同磷酸化状态的蛋白质来评估肝细胞癌的方法; 和用于检测由蛋白质形成的肝细胞癌的肝细胞癌蛋白标记物。 用于检测肝细胞癌的肝细胞癌蛋白标志物包括由SEQ ID NO:1表示的氨基酸形成的肿瘤排斥抗原gp96,并测量其磷酸化状态以检测肝细胞癌的存在或不存在。

    Conductive paste and wiring board using same
    14.
    发明授权
    Conductive paste and wiring board using same 失效
    导电膏和使用其的接线板

    公开(公告)号:US08053066B2

    公开(公告)日:2011-11-08

    申请号:US11887844

    申请日:2007-01-18

    IPC分类号: B32B7/02

    摘要: A conductive paste contains metal powder, an inorganic binder, and an organic vehicle as main ingredients. The organic vehicle includes a solvent having a boiling point of 270° C. or higher, and the proportion of the solvent with respect to the entire solvent included in the organic vehicle is 3 to 100% by weight.

    摘要翻译: 导电糊包含金属粉末,无机粘合剂和有机载体作为主要成分。 有机载体包括沸点为270℃以上的溶剂,溶剂相对于有机载体中所含的全部溶剂的比例为3〜100重量%。

    Verification apparatus and verification method for recording media
    15.
    发明授权
    Verification apparatus and verification method for recording media 失效
    用于记录媒体的验证装置和验证方法

    公开(公告)号:US07778540B2

    公开(公告)日:2010-08-17

    申请号:US11790782

    申请日:2007-04-27

    摘要: On a recording medium serving as a verification object, a picture whose state changes in accordance with optical conditions is formed. A verification apparatus images a plurality of pictures of the recording medium while changing an optical relationship among a light source for illuminating the recording medium, a camera for imaging a picture of the recording medium, and the recording medium with a condition modifying unit. The verification apparatus displays the plurality of pictures of the recording medium imaged by the camera, on the display unit in a state in which the optical relationship has been changed. The verification apparatus determines imaging conditions for imaging a picture for verification on the basis of the optical relationship at the time of photographing selected pictures among the plurality of pictures displayed on the display unit.

    摘要翻译: 在用作验证对象的记录介质上,形成其状态根据光学条件而变化的图像。 验证装置在改变用于照亮记录介质的光源之间的光学关系,用于对记录介质的图像进行成像的相机和具有条件修改单元的记录介质之间的光学关系的同时,对记录介质的多个图像进行成像。 验证装置在光学关系已经改变的状态下在显示单元上显示由相机成像的记录介质的多张图像。 验证装置基于在显示单元上显示的多个图像中拍摄所选择的图像时的光学关系,确定用于对图像进行成像的成像条件。

    Method of analyzing C-terminal amino acid sequence of peptide
    16.
    发明授权
    Method of analyzing C-terminal amino acid sequence of peptide 失效
    分析肽的C-末端氨基酸序列的方法

    公开(公告)号:US07670841B2

    公开(公告)日:2010-03-02

    申请号:US10536824

    申请日:2003-11-28

    IPC分类号: G01N33/00

    CPC分类号: G01N33/6842 G01N33/6821

    摘要: The present invention provides, as a method of analyzing the C-terminal amino acid sequence of a peptide with use of reaction technique for successively releasing the C-terminal amino acids, in which undesirable side reactions, such as cleavage of a peptide bond at the middle of the peptide, can be prevented and chemical treatments therein can be carried out under widely applicable conditions in the course of successive release of the C-terminal amino acids from a peptide, such a method comprising steps of dehydrating the gel on which a target peptide that has been separated by gel electrophoresis is held in the bound state; immersing it in a mixture solution of an alkanoic acid anhydride added with a small amount of a perfluoroalkanoic acid in a dipolar aprotic solvent to re-swell the gel carrier, forming a 5-oxazolone structure, at a temperature chosen in the range of from 30° C. to 80° C., followed by the cleavage of the 5-oxazolone ring to release the C-terminal amino acids, and then specifying the C-terminal amino acid sequence of the peptide based on the measured decrease in the molecular weight of a series of reaction products resulting therefrom.

    摘要翻译: 本发明提供了使用用于连续释放C-末端氨基酸的反应技术分析肽的C-末端氨基酸序列的方法,其中不期望的副反应,例如在 肽的中间,可以在广泛适用的条件下在肽的C末端氨基酸连续释放的过程中进行其中的化学处理,这种方法包括使凝胶脱水,其中将目标 通过凝胶电泳分离的肽保持在结合状态; 将其加入到加入少量全氟链烷酸的链烷酸与偶极非质子传递溶剂的混合溶液中,使溶胶载体重新溶胀,形成5-恶唑酮结构,温度范围为30℃ ℃至80℃,然后切割5-恶唑酮环以释放C末端氨基酸,然后根据测定的分子量减少指定肽的C-末端氨基酸序列 的一系列由此产生的反应产物。

    Personal authentication medium issuing method
    17.
    发明授权
    Personal authentication medium issuing method 有权
    个人认证介质发行方式

    公开(公告)号:US07243843B2

    公开(公告)日:2007-07-17

    申请号:US10995540

    申请日:2004-11-24

    IPC分类号: G06K5/00

    摘要: In a personal authentication medium issuing apparatus, a main image information is input and whether a personal authentication medium on which synthesized image information having sub-information embedded in the main image information is checked or not is determined based on the state of the input main image information. Further, synthesized image information having sub-information embedded in an invisible state in the main image information which is set in a visible state is formed, a personal authentication medium is formed by printing the synthesized image information on a recording medium and the thus formed personal authentication media are sorted into personal authentication media determined to be completely formed and personal authentication media determined to require a checking process.

    摘要翻译: 在个人认证介质发行装置中,输入主图像信息,并且基于输入主图像的状态来确定是否检测到具有嵌入在主图像信息中的子信息的合成图像信息被检查的个人认证介质 信息。 此外,形成具有嵌入在可见状态的主图像信息中的不可见状态的子信息的合成图像信息,通过将合成图像信息印刷在记录介质上形成个人认证介质,并且由此形成的个人 认证介质被分类为确定为完全形成的个人认证介质,并且确定要求检查过程的个人认证介质。

    Method for analyzing c-terminal amono acid sequence of peptide
    19.
    发明申请
    Method for analyzing c-terminal amono acid sequence of peptide 失效
    分析肽末端氨基酸序列的方法

    公开(公告)号:US20060057731A1

    公开(公告)日:2006-03-16

    申请号:US10538305

    申请日:2003-12-04

    IPC分类号: G01N33/00

    CPC分类号: G01N33/6821 G01N33/6842

    摘要: The present invention provides, as for a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions, a following method wherein a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products by analysis in negative mode of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

    摘要翻译: 本发明提供了通过使用连续释放肽的C末端氨基酸的反应来分析肽的C末端氨基酸序列的方法,该方法可以在连续释放C端 具有长氨基酸长度的肽的氨基酸,这种不利的副反应,如在肽的中间位置处的肽键断裂,并且可以在广泛适用的条件下进行化学处理,以下方法,其中将 具有长氨基酸长度的肽预先进行N-酰化处理; 通过使用其中链烷酸酐与少量全氟链烷酸组合的反应试剂,在温和条件下连续释放C-末端氨基酸; 应用水解处理; 然后通过胰蛋白酶消化进行精氨酸残基位点的选择性碎裂; 此后,通过MALDI-TOF-MS装置的负模式的分析,测量衍生自一系列反应产物的C末端侧片段的分子量的降低; 鉴定出肽样品的C-末端氨基酸序列。

    Board for printed wiring, printed wiring board, and method for manufacturing them
    20.
    发明申请
    Board for printed wiring, printed wiring board, and method for manufacturing them 审中-公开
    印刷电路板,印刷线路板及其制造方法

    公开(公告)号:US20050236182A1

    公开(公告)日:2005-10-27

    申请号:US10517058

    申请日:2003-06-02

    摘要: A novel board for printed wiring comprising a fine conductor wiring having a clear and favorable boundary line and fabricated by an ordinal printing method such as screen printing, a printed wiring board using the same, and methods for manufacturing them. A board for printed wiring and a method for manufacturing the same are characterized in that the surface of a board is subjected to one of the surface treatments: (a) roughening, (2) plasma treatment, (3) roughening and then plasma treatment, and (4) roughening and then forming of a metal film coating by sputtering. A printed wiring board and a method for manufacturing the same is characterize in that a conductor wiring is fabricated by printing using a conductive paste containing metal particles the average particle diameter of which is 4 μm or less and the maximum particle diameter of which is 15 μm or less. Another printed wiring board and a method for manufacturing the same is characterized in that the surface of a conductor wiring fabricated using a conductive paste containing metal particles M and a binder B at a volume ratio of M/B of 1/1 to 1.9/1 is etched, a plating coating is formed on the surface.

    摘要翻译: 一种用于印刷布线的新型印刷电路板,包括具有透明且有利的边界线的精细导体布线,并通过诸如丝网印刷的顺序印刷法,使用其的印刷线路板及其制造方法制造。 印刷电路板及其制造方法的特征在于,对板的表面进行表面处理之一:(a)粗糙化,(2)等离子体处理,(3)粗糙化,然后进行等离子体处理, 和(4)通过溅射粗化然后形成金属膜涂层。 印刷电路板及其制造方法的特征在于,使用含有平均粒径为4μm以下,最大粒径为15μm的金属粒子的导电糊进行印刷,制造导体布线 或更少。 另一种印刷线路板及其制造方法的特征在于,使用含有金属粒子M和粘合剂B的M / B的体积比为1/1〜1.9 / 1的导电性糊剂制造的导体布线的表面 被蚀刻,在表面上形成镀覆层。