公开(公告)号：US20190055538A1

公开(公告)日：2019-02-21

申请号：US16179311

申请日：2018-11-02

申请人： Siemens Healthcare Diagnostics Inc.

发明人： Yi Feng Zheng ,  Yali Yang ,  Linda Hudson ,  Nihmat Morjana

IPC分类号： C12N9/96 ,  G01N33/58 ,  C12N9/04 ,  G01N33/535

CPC分类号： C12N9/96 ,  C07K2319/00 ,  C12N9/0006 ,  C12Y101/01049 ,  G01N33/535 ,  G01N33/581

摘要： The present invention relates to multi-hapten mutant G6PDH conjugates, methods of their preparation and their use for detection of multiple analytes. The compositions of the invention comprise different types of haptens (molecules) require to be immobilized into the G6PDH to make one multi-hapten-G6PDH conjugate. Both thiol and amine functional groups on G6PDH are utilized for immobilization of different haptens.

公开(公告)号：US20160231336A1

公开(公告)日：2016-08-11

申请号：US15131120

申请日：2016-04-18

申请人： Siemens Healthcare Diagnostics Inc.

发明人： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

IPC分类号： G01N33/78 ,  G01N33/58

CPC分类号： G01N33/78 ,  G01N33/582 ,  G01N2333/59

摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

摘要翻译： 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 该组合经受用于将分析物与分析物的结合配偶体结合的条件。 敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

公开(公告)号：US09121859B2

公开(公告)日：2015-09-01

申请号：US13693229

申请日：2012-12-04

申请人： Siemens Healthcare Diagnostics Inc.

发明人： Yi Feng Zheng ,  Tie Q. Wei

IPC分类号： G01N33/53 ,  A61K31/436 ,  G01N33/94

CPC分类号： G01N33/9493 ,  A61K31/436 ,  G01N33/5308

摘要： Compositions are disclosed for releasing an FKBP-binding immunosuppressant drug from endogenous binding substances in a sample suspected of containing the FKBP-binding immunosuppressant drug. The compositions include sirolimus derivatives that are modified with a bulky organic radical in the triene portion of the sirolimus molecule. The compositions may be employed in conjunction with assays for an FKBP-binding immunosuppressant drug in a sample suspected of containing the drug.

摘要翻译： 公开了用于从怀疑含有FKBP结合免疫抑制剂药物的样品中的内源性结合物质释放FKBP结合免疫抑制剂药物的组合物。 组合物包括在西罗莫司分子的三烯部分用大体积有机基团修饰的西罗莫司衍生物。 组合物可以结合用于怀疑含有该药物的样品中的FKBP结合免疫抑制剂药物的测定。

公开(公告)号：US20140154706A1

公开(公告)日：2014-06-05

申请号：US13693229

申请日：2012-12-04

申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC.

发明人： Yi Feng Zheng ,  Tie Q. Wei

IPC分类号： G01N33/94

CPC分类号： G01N33/9493 ,  A61K31/436 ,  G01N33/5308

摘要： Compositions are disclosed for releasing an FKBP-binding immunosuppressant drug from endogenous binding substances in a sample suspected of containing the FKBP-binding immunosuppressant drug. The compositions include sirolimus derivatives that are modified with a bulky organic radical in the triene portion of the sirolimus molecule. The compositions may be employed in conjunction with assays for an FKBP-binding immunosuppressant drug in a sample suspected of containing the drug.

摘要翻译： 公开了用于从怀疑含有FKBP结合免疫抑制剂药物的样品中的内源性结合物质释放FKBP结合免疫抑制剂药物的组合物。 组合物包括在西罗莫司分子的三烯部分用大体积有机基团修饰的西罗莫司衍生物。 组合物可以结合用于怀疑含有该药物的样品中的FKBP结合免疫抑制剂药物的测定。