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1.发明授权公开(公告)号：US10450555B2
公开(公告)日：2019-10-22
申请号：US16179311
申请日：2018-11-02
发明人： Yi Feng Zheng , Yali Yang , Linda Hudson , Nihmat Morjana
IPC分类号： C12N9/96 , C12N9/04 , G01N33/535 , G01N33/58
摘要： The present invention relates to multi-hapten mutant G6PDH conjugates, methods of their preparation and their use for detection of multiple analytes. The compositions of the invention comprise different types of haptens (molecules) require to be immobilized into the G6PDH to make one multi-hapten-G6PDH conjugate. Both thiol and amine functional groups on G6PDH are utilized for immobilization of different haptens.
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2.发明申请PREPARATION OF MULTI-HAPTEN MUTANT G6PDH CONJUGATES AND THEIR USE FOR DETECTION OF MULTIPLE ANALYTES 审中-公开多发性突变体G6PDH结合体的制备及其用于检测多种分析物公开(公告)号：US20160312208A1
公开(公告)日：2016-10-27
申请号：US15103572
申请日：2014-12-10
发明人： Yi Feng Zheng , Yali Yang , Linda Hudson , Nihmat Morjana
CPC分类号： C12N9/96 , C07K2319/00 , C12N9/0006 , C12Y101/01049 , G01N33/535 , G01N33/581
摘要： The present invention relates to multi-hapten mutant G6PDH conjugates, methods of their preparation and their use for detection of multiple analytes. The compositions of the invention comprise different types of haptens (molecules) require to be immobilized into the G6PDH to make one multi-hapten-G6PDH conjugate. Both thiol and amine functional groups on G6PDH are utilized for immobilization of different haptens.
摘要翻译： 本发明涉及多重半抗原突变体G6PDH缀合物，其制备方法及其用于检测多种分析物的用途。 本发明的组合物包含需要固定到G6PDH中的不同类型的半抗原（分子）以制备一个多重半抗原-G6PDH缀合物。 G6PDH上的硫醇和胺官能团都用于固定不同的半抗原。
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公开(公告)号：US20170362579A1
公开(公告)日：2017-12-21
申请号：US15536279
申请日：2015-12-11
发明人： Yi Feng Zheng , Yali Yang , Guoping Wang
IPC分类号： C12N9/04 , G01N33/58 , G01N33/569 , G01N33/535 , C12Q1/28 , C07K16/44 , C07D405/12 , G01N33/94 , C07D317/58
CPC分类号： C12N9/0006 , C07D317/58 , C07D405/12 , C07D489/02 , C07D489/08 , C07K16/44 , C12Q1/28 , G01N33/535 , G01N33/56911 , G01N33/581 , G01N33/94 , G01N33/9486
摘要： Compositions, methods, and kits are disclosed directed at haptens, immunogens and immunoassays for oxycodone and metabolites thereof. The compounds are exemplified by compounds of the Formula I. The method comprises providing in combination in a medium (i) a sample suspected of containing oxycodone and/or oxycodone metabolites, a compound of the Formula I wherein R4 or R5 is a label, and an antibody for oxycodone or a metabolite thereof. The medium is examined for the presence of a complex comprising the labeled compound of Formula I where the presence of such as complex indicates the presence of oxycodone or oxycodone metabolite in the sample.
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4.发明公开公开(公告)号：US20240010841A1
公开(公告)日：2024-01-11
申请号：US18255654
申请日：2021-11-10
发明人： Yali Yang , David Wen , Bogdan Draghici , Yi Feng Zheng , Raphael Bartz , Qingping Jiang , Roland Janzen , Joshua Whalen , Ling Ngo , William Bedzyk
CPC分类号： C09B62/125 , G01N33/582 , C07K16/28 , G01N33/6872 , G01N2333/99
摘要： Labels that include dibromopyridazinedione attached to signal molecules are disclosed, along with methods of producing and using same. Also disclosed are conjugates of the label attached to an analyte-specific binder, as well as methods of producing and using same. Kits containing the labels and/or conjugates are also disclosed, along with microfluidics devices containing same.
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公开(公告)号：US20170362305A1
公开(公告)日：2017-12-21
申请号：US15536296
申请日：2015-12-11
发明人： Yi Feng Zheng , Tie Q. Wei , Manoj Sharma
IPC分类号： C07K16/00 , G01N33/566 , G01N33/94 , C07D497/04
CPC分类号： C07K16/00 , C07D497/04 , C07K16/44 , C07K2317/10 , C07K2317/54 , G01N33/566 , G01N33/9493
摘要： Methods are disclosed of designing antibodies for a sandwich assay for a small molecule having a molecular weight of about 500 to about 2,000. The method comprises preparing a first antibody that binds to the small molecule, and preparing a second antibody that binds to the small molecule at a portion of the small molecule other than a portion to which the first antibody binds. The second antibody is prepared from an immunogen that comprises a predetermined portion of the small molecule. The antibodies may be employed in sandwich assays for the small molecule.
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公开(公告)号：US20210003596A1
公开(公告)日：2021-01-07
申请号：US16488524
申请日：2018-02-23
发明人： Yi Feng Zheng , Lauren Parker , John Driscoll , Zhijian Zhao , Patrick Donovan
摘要： Chemiluminescent androstenedione conjugates are disclosed. These chemiluminescent androstenedione conjugates may be used as chemiluminescent tracers in immunoassays for the quantification and identification of certain analytes.
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公开(公告)号：US09720004B2
公开(公告)日：2017-08-01
申请号：US15131120
申请日：2016-04-18
发明人： Pratap Singh , Yi Feng Zheng , Liping Geng , Roland Janzen , Carsten Schelp
CPC分类号： G01N33/78 , G01N33/582 , G01N2333/59
摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.
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公开(公告)号：US11377483B2
公开(公告)日：2022-07-05
申请号：US17247219
申请日：2020-12-04
发明人： Yi Feng Zheng , Tie Q. Wei , Manoj Sharma
IPC分类号： C07K16/00 , G01N33/94 , G01N33/566 , C07K16/44 , C07D497/04
摘要： Methods are disclosed of designing antibodies for a sandwich assay for a small molecule having a molecular weight of about 500 to about 2,000. The method comprises preparing a first antibody that binds to the small molecule, and preparing a second antibody that binds to the small molecule at a portion of the small molecule other than a portion to which the first antibody binds. The second antibody is prepared from an immunogen that comprises a predetermined portion of the small molecule. The antibodies may be employed in sandwich assays for the small molecule.
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公开(公告)号：US20210087255A1
公开(公告)日：2021-03-25
申请号：US17247219
申请日：2020-12-04
发明人： Yi Feng Zheng , Tie Q. Wei , Manoj Sharma
IPC分类号： C07K16/00 , G01N33/94 , C07K16/44 , G01N33/566
摘要： Methods are disclosed of designing antibodies for a sandwich assay for a small molecule having a molecular weight of about 500 to about 2,000. The method comprises preparing a first antibody that binds to the small molecule, and preparing a second antibody that binds to the small molecule at a portion of the small molecule other than a portion to which the first antibody binds. The second antibody is prepared from an immunogen that comprises a predetermined portion of the small molecule. The antibodies may be employed in sandwich assays for the small molecule.
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公开(公告)号：US10351830B2
公开(公告)日：2019-07-16
申请号：US15536279
申请日：2015-12-11
发明人： Yi Feng Zheng , Yali Yang , Guoping Wang
IPC分类号： G01N33/58 , G01N33/94 , G01N33/535 , C07K16/44 , C07D317/58 , C12N9/04 , C07D489/02 , C07D489/08 , C07D405/12 , C12Q1/28 , G01N33/569
摘要： Compositions, methods, and kits are disclosed directed at haptens, immunogens and immunoassays for oxycodone and metabolites thereof. The compounds are exemplified by compounds of the Formula I. The method comprises providing in combination in a medium (i) a sample suspected of containing oxycodone and/or oxycodone metabolites, a compound of the Formula I wherein R4 or R5 is a label, and an antibody for oxycodone or a metabolite thereof. The medium is examined for the presence of a complex comprising the labeled compound of Formula I where the presence of such as complex indicates the presence of oxycodone or oxycodone metabolite in the sample.
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