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公开(公告)号：US11295833B2
公开(公告)日：2022-04-05
申请号：US17042108
申请日：2019-03-19
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba
Abstract: A mass isolation device selects a precursor ion of a sample that has been digested using a protease. A first fragmentation device fragments the precursor ion using collision-induced dissociation (CID), and the resulting product ions are analyzed using a mass analyzer producing a CID spectrum. A list of theoretical candidate glycopeptide sequences is determined from CID spectrum. The mass isolation device again selects the precursor ion of the sample. A second fragmentation device fragments the precursor ion using electron-based dissociation (ExD), and the resulting product ions are analyzed using the mass analyzer producing a CID spectrum. For each sequence of the list, the sequence is computationally fragmented, producing theoretical fragments, mass-to-charge ratio (m/z) values are calculated for the theoretical fragments, and the sequence is scored using c and z fragment matching rules. The highest scoring sequence is identified as a peptide sequence of a glycopeptide of the sample.
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公开(公告)号：US11002744B2
公开(公告)日：2021-05-11
申请号：US16626732
申请日：2018-06-28
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Takashi Baba , Yves Le Blanc
Abstract: A system and method is described for characterizing glycopeptides which includes a first quadrupole mass filter, a multipole rod set of an ion guide, a lens electrode, an ExD device and a mass analyzer. The multipole rod set is adapted to receive a radial radio frequency (RF) trapping voltage and a radial dipole direct current (DC) voltage The lens electrode is adapted to receive an axial trapping alternating current (AC) voltage and a DC voltage. The ExD device performs electron capture dissociation or electron transfer dissociation, the ExD device being positioned so that an entrance of the ExD device is disposed on the other side of the lens electrode opposite the multipole rod set. The mass analyzer is positioned at an exit of the ExD device for receiving ions from the ExD device.
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公开(公告)号：US10541124B2
公开(公告)日：2020-01-21
申请号：US16069506
申请日：2017-01-10
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Takashi Baba
Abstract: Improvements to a side-on Penning trap include methods to stabilize ions in the trap. The ions are stabilized by injecting ions in the focusing region of the non-uniform DC fields produced by the pad electrodes of the trap. Ions are injected along an injection axis shifted from the central axis of a gap between a positively biased electrode pad and negatively biased electrode pad of the trap. Improvements also include methods to compensate for the Lorentz force that is produced when ions are injected into a side-on Penning trap. Electrodes of an ion injection device are DC biased so that the electrodes produce an electric field along the axis of the device that compensates for the Lorentz force. Finally, methods are provided to increase the m/z range of ions injected into a side-on Penning trap by pre-trapping ions just before injection of the ions into the trap.
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公开(公告)号：US10147593B2
公开(公告)日：2018-12-04
申请号：US15513271
申请日：2015-10-14
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Takashi Baba
IPC: H01J49/26 , B01D59/48 , H01J49/02 , H01J49/48 , H01J37/317
Abstract: A static magnetic field is applied in the same direction using a first magnet and a second magnet. The magnetic field is applied to a gap of uniform thickness between the first and second magnets. Voltages are applied to three or more electrodes located in the gap using a switchable voltage source. The voltages are applied to create a static electric field perpendicular to the static magnetic field in an input channel and at least one channel of two or more output channels. The channels are defined by gaps between the three or more electrodes. The simultaneous application of the static magnetic field and the static electric field causes ions from an ion beam of a mass spectrometer to move into and through the input channel and through at least one channel of the two or more output channels to another location in the mass spectrometer.
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公开(公告)号：US10068752B2
公开(公告)日：2018-09-04
申请号：US15026235
申请日：2014-10-07
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Takashi Baba
Abstract: Systems and methods for multiplexed precursor ion selection are provided. A mass isolator includes a selection region of rods, a transmission region of rods, and a barrier electrode lens separating the selection and transmission regions. Two or more different precursor ions are selected by applying two or more different AC voltage frequencies to rods of a selection region in order to resonate the two or more different precursor ions from a continuous beam of ions. The two or more different precursor ions are transmitted by applying a DC voltage to the barrier electrode lens, creating an electric field potential barrier over which only the resonating ions are transmitted. Precursor ions of product ions from combined product ion spectra produced by multiplexed precursor ion selection are identified by grouping the target precursor ions.
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Patent Agency Ranking
16.发明申请公开(公告)号：US20170221692A1
公开(公告)日：2017-08-03
申请号：US15329376
申请日：2015-07-24
Applicant: DH Technologies Development PTE Ltd.
Inventor： Takashi Baba
CPC classification number: H01J49/403 , H01J49/063 , H01J49/067 , H01J49/429
Abstract: A multipole rod set of an ion guide is adapted to receive a radial RF trapping voltage and a radial dipole direct current DC voltage. A lens electrode of the ion guide is positioned at one end of the multipole rod set to extract ions from the multipole rod set and adapted to receive an axial trapping AC voltage and a DC voltage. A radial dipole DC voltage is applied to the multipole rod set and an axial trapping AC voltage is simultaneously applied to a lens electrode in order to extract a bandpass mass range of ions trapped in the multipole rod set. Alternatively, a radial RF trapping voltage amplitude is applied to the multipole rod set and an axial trapping AC voltage is simultaneously applied to the lens electrode in order to extract a bandpass mass range of ions trapped in the multipole rod set.
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公开(公告)号：US20160336160A1
公开(公告)日：2016-11-17
申请号：US15106865
申请日：2014-11-18
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： Takashi Baba
IPC: H01J49/06
CPC classification number: H01J49/062 , H01J49/06
Abstract: Methods and systems for transmitting ions in an ion guide are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can cause at least a portion of ions entrained in a gas flow entering an ion guide to be extracted from the gas jet and be guided downstream along one or more path of gas flow, where the gas lacking the ions can be removed from the ion guide. In some embodiments, the ions extracted from the gas stream can be guided into a focusing region in which the ions can be focused, e.g., via RF focusing, to enter into subsequence processing stages, such as a mass analyzer.
Abstract translation: 本文提供了用于在离子导向器中传输离子的方法和系统。 根据申请人的教导的各个方面，所述方法和系统可以使夹带在进入离子导向器的气流中的至少一部分离子从气体射流中提取出来，并沿气流的一个或多个路径向下游引导 ，其中可以从离子导向器去除缺乏离子的气体。 在一些实施方案中，从气流提取的离子可以被引导到聚焦区域中，其中离子可以例如通过RF聚焦被聚焦，以进入子序列处理阶段，例如质量分析器。
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公开(公告)号：US09287103B2
公开(公告)日：2016-03-15
申请号：US14431606
申请日：2013-10-11
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Takashi Baba
CPC classification number: H01J49/062 , H01J49/0031 , H01J49/067 , H01J49/22
Abstract: An ion guide is provided having an enclosure extending longitudinally around a central axis from a proximal inlet end to a distal outlet end. The proximal inlet end receives a plurality of ions entrained in a gas flow through an inlet orifice. A deflection plate is disposed within the enclosure between the proximal and distal ends and deflects at least a portion of the gas flow away from a central direction of the gas flow. A plurality of electrically conductive, elongate elements extend from the proximal end to the distal end within the enclosure and generate an electric field via a combination of RF and DC electric potentials. The electric field deflects the entrained ions away from the central direction of the gas flow proximal to the deflection plate and confines the deflected ions in proximity of the elongated elements as the ions travel downstream.
Abstract translation: 提供离子引导件，其具有围绕中心轴线从近端入口端部至远端出口端部纵向延伸的外壳。 近端入口端容纳夹带在通过入口孔的气流中的多个离子。 偏转板在近端和远端之间的外壳内设置，并使气流的至少一部分偏离气流的中心方向。 多个导电细长元件从外壳内的近端延伸到远端，并通过RF和DC电势的组合产生电场。 电场使夹带的离子偏离靠近偏转板的气流的中心方向，并且当离子向下游行进时，将偏转的离子限制在细长元件附近。
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公开(公告)号：US09177771B2
公开(公告)日：2015-11-03
申请号：US14368703
申请日：2012-11-28
Applicant: DH Technologies Development Pte. Ltd.
Inventor： Takashi Baba
CPC classification number: H01J49/063 , H01J49/062 , H01J49/36
Abstract: A method and apparatus is provided including an ion source for generating ions, a vacuum chamber having an inlet aperture for receiving ions and an exit aperture for passing ions from the vacuum chamber. At least one ion guide is provided between the inlet and exit apertures, the at least one ion guide having an entrance end and an exit end. The at least one ion guide having an inner cylinder and an outer cylinder. The inner cylinder having a plurality of sections, each section having a different number of slots and the inner cylinder coaxially disposed within the outer cylinder wherein the inner cylinder is configured to generate more than one multipole field. A power supply is provided for providing an RF voltage between the outer and inner cylinders.
Abstract translation: 提供了一种方法和装置，包括用于产生离子的离子源，具有用于接收离子的入口孔的真空室和用于使来自真空室的离子的出口孔。 至少一个离子导向件设置在入口孔和出口孔之间，所述至少一个离子导向件具有入口端和出口端。 所述至少一个离子导向件具有内筒和外筒。 所述内筒具有多个部分，每个部分具有不同数量的槽，并且所述内筒同轴地设置在所述外筒内，其中所述内筒被配置为产生多于一个多极场。 提供电源以在外筒和内筒之间提供RF电压。
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公开(公告)号：US20150279647A1
公开(公告)日：2015-10-01
申请号：US14431606
申请日：2013-10-11
Applicant: DH Technologies Development PTE. LTD.
Inventor： Takashi Baba
CPC classification number: H01J49/062 , H01J49/0031 , H01J49/067 , H01J49/22
Abstract: An ion guide is provided having an enclosure extending longitudinally around a central axis from a proximal inlet end to a distal outlet end. The proximal inlet end receives a plurality of ions entrained in a gas flow through an inlet orifice. A deflection plate is disposed within the enclosure between the proximal and distal ends and deflects at least a portion of the gas flow away from a central direction of the gas flow. A plurality of electrically conductive, elongate elements extend from the proximal end to the distal end within the enclosure and generate an electric field via a combination of RF and DC electric potentials. The electric field deflects the entrained ions away from the central direction of the gas flow proximal to the deflection plate and confines the deflected ions in proximity of the elongated elements as the ions travel downstream.
Abstract translation: 提供离子引导件，其具有围绕中心轴线从近端入口端部至远端出口端部纵向延伸的外壳。 近端入口端容纳夹带在通过入口孔的气流中的多个离子。 偏转板在近端和远端之间的外壳内设置，并使气流的至少一部分偏离气流的中心方向。 多个导电细长元件从外壳内的近端延伸到远端，并通过RF和DC电势的组合产生电场。 电场使夹带的离子偏离靠近偏转板的气流的中心方向，并且当离子向下游行进时，将偏转的离子限制在细长元件附近。
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