BIOLOGICAL INDICATOR
    12.
    发明申请
    BIOLOGICAL INDICATOR 审中-公开
    生物指标

    公开(公告)号:US20160312232A1

    公开(公告)日:2016-10-27

    申请号:US15204079

    申请日:2016-07-07

    Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

    Abstract translation: 本发明涉及一种衍生自组合物的生物指示剂,其包含:宿主生物,其包含孢子形成细菌; 用于产生指示酶的报告基因; 调节基因; 以及用于将报道基因和调节基因插入宿主生物体的载体; 宿主生物体在其基因组中具有转座遗传元件,用于将插入序列插入调节基因; 所述插入序列包含转座酶,一对末端反向重复序列和用于表达转座酶的至少一个开放阅读框。 载体可能被宿主生物体吸收。 插入序列可插入调节基因。 宿主生物体可能经历孢子形成以形成生物指示剂。 公开了一种使用生物指示剂的方法和装置。

    Biological indicator
    13.
    发明授权
    Biological indicator 有权
    生物指标

    公开(公告)号:US09416393B2

    公开(公告)日:2016-08-16

    申请号:US14512708

    申请日:2014-10-13

    Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

    Abstract translation: 本发明涉及一种衍生自组合物的生物指示剂,其包含:宿主生物,其包含孢子形成细菌; 用于产生指示酶的报告基因; 调节基因; 以及用于将报道基因和调节基因插入宿主生物体的载体; 宿主生物体在其基因组中具有转座遗传元件,用于将插入序列插入到调节基因中; 所述插入序列包含转座酶,一对末端反向重复序列和用于表达转座酶的至少一个开放阅读框。 载体可能被宿主生物体吸收。 插入序列可插入调节基因。 宿主生物体可能经历孢子形成以形成生物指示剂。 公开了一种使用生物指示剂的方法和装置。

    Process and composition for killing spores

    公开(公告)号:US10750749B2

    公开(公告)日:2020-08-25

    申请号:US14262840

    申请日:2014-04-28

    Abstract: This invention relates to a process and an aqueous composition for killing spores. The process comprises contacting the spores with the aqueous composition for a sufficient period of time to effect a desired reduction (e.g., at least a 4 log reduction) in the number of spores capable of reproduction, metabolism and/or growth. The aqueous composition comprises water, peracetic acid and hydrogen peroxide. The concentration of peracetic acid in the water may be in the range from about 0.001 to about 60% by weight, or from about 0.001 to about 0.5% by weight. The weight ratio of peracetic acid to hydrogen peroxide may be in the range from about 0.001 to about 0.5.

    BIOLOGICAL INDICATOR
    20.
    发明申请

    公开(公告)号:US20160102335A1

    公开(公告)日:2016-04-14

    申请号:US14512708

    申请日:2014-10-13

    Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

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