公开(公告)号：US20100240103A1

公开(公告)日：2010-09-23

申请号：US12646514

申请日：2009-12-23

Applicant: Fei Mao ,  Xing Xin

Inventor： Fei Mao ,  Xing Xin

IPC: C12P19/34 ,  C07H21/00

CPC classification number: C12Q1/6818 ,  C12Q1/6851 ,  Y10S435/975 ,  C12Q2565/1015 ,  C12Q2561/101 ,  C12Q2565/1025

Abstract: An embodiment of the invention discloses new methods for designing labeled nucleic acid probes and primers by labeling oligonucleotides with a plurality of spectrally identical or similar dyes and optionally with one or more quencher dyes. Oligonucleotides labeled in accordance with some embodiments of the invention exhibit a detectable increase in signal, for example, fluorescent signal when the labeling dyes are separated from one another. Methods for separating the dye include cleaving the labeled oligonucleotides include using enzymes that have 5′-exonuclease activity. In one embodiment nucleic acid primers of the present invention may fluoresce upon hybridization to a target sequence and incorporation into the amplification product. Nucleic acid probes and primers of the present invention have wide applications ranging from general detection of a target nucleic acid sequence to clinical diagnostics. Major advantages of the oligonucleotides including nucleic acid probes and primers of many embodiments of the present invention are their synthetic simplicity, spectral versatility and superior fluorescent signal.

Abstract translation: 本发明的一个实施方案公开了通过用多个光谱相同或相似的染料和任选地与一种或多种猝灭剂染料标记寡核苷酸来设计标记的核酸探针和引物的新方法。 根据本发明的一些实施方案标记的寡核苷酸在标记染料彼此分离时表现出可检测的信号增加，例如荧光信号。 用于分离染料的方法包括切割标记的寡核苷酸包括使用具有5'-外切核酸酶活性的酶。 在一个实施方案中，本发明的核酸引物可以在与靶序列杂交时发荧光，并掺入扩增产物。 本发明的核酸探针和引物具有广泛的应用范围，从靶核酸序列的一般检测到临床诊断。 包括本发明许多实施方案的核酸探针和引物的寡核苷酸的主要优点是它们的合成简便性，光谱通用性和优异的荧光信号。

公开(公告)号：US07776567B2

公开(公告)日：2010-08-17

申请号：US11377253

申请日：2006-03-16

Applicant: Fei Mao ,  Wai-Yee Leung ,  Xing Xin

Inventor： Fei Mao ,  Wai-Yee Leung ,  Xing Xin

IPC: C12P19/34 ,  C12Q1/68 ,  C07H19/04 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6876 ,  C07D209/08 ,  C07D213/38 ,  C07D221/10 ,  C07D417/06 ,  C07D417/14 ,  C07D491/147 ,  C07H21/00 ,  C09B15/00 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2563/173

Abstract: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.

Abstract translation: 提供二聚和三聚体核酸染料以及相关系统和方法。 这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。 在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。 本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

公开(公告)号：US08354523B2

公开(公告)日：2013-01-15

申请号：US12307358

申请日：2007-07-19

Applicant: Fei Mao ,  Xing Xin

Inventor： Fei Mao ,  Xing Xin

IPC: C07H19/04 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C07H19/04 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6851 ,  C12Q2561/101 ,  C12Q2527/107

Abstract: The present invention provides a dually labeled oligonucleotide probe, methods of preparing and using the same. The subject probes are particularly useful for high-sensitive nucleic acid detection via hybridization assays including but not limited to template-directed polymerization reactions.

Abstract translation: 本发明提供双重标记的寡核苷酸探针，其制备和使用方法。 本发明探针通过杂交测定法对于高敏感性核酸检测特别有用，包​​括但不限于模板指导的聚合反应。

公开(公告)号：US20110201010A1

公开(公告)日：2011-08-18

申请号：US13001735

申请日：2009-07-02

Applicant: Fei Mao ,  Xing Xin ,  Wai-Lee Leung

Inventor： Fei Mao ,  Xing Xin ,  Wai-Lee Leung

IPC: C12Q1/68 ,  C12N9/99 ,  C12P19/34 ,  C12N9/12 ,  C12M1/34

CPC classification number: C12Q1/6844 ,  C07K1/064 ,  C08H1/00 ,  C12N9/1252 ,  C12N9/127 ,  C12P19/34 ,  C12Q2521/101 ,  C12Q2521/119

Abstract: The present invention provides compositions, methods, and kits relating to the protection and deprotection of molecules comprising nucleophilic groups, such as the protection and deprotection of thermostable polymerases. Also provided are methods of performing nucleic acid amplification using polymerases protected according to the invention.

Abstract translation: 本发明提供了与包含亲核基团的分子的保护和去保护有关的组合物，方法和试剂盒，例如耐热聚合酶的保护和去保护。 还提供使用根据本发明保护的聚合酶进行核酸扩增的方法。

公开(公告)号：US20060211028A1

公开(公告)日：2006-09-21

申请号：US11377253

申请日：2006-03-16

Applicant: Fei Mao ,  Wai-Yee Leung ,  Xing Xin

Inventor： Fei Mao ,  Wai-Yee Leung ,  Xing Xin

IPC: C12Q1/68 ,  C07D401/02

CPC classification number: C12Q1/6876 ,  C07D209/08 ,  C07D213/38 ,  C07D221/10 ,  C07D417/06 ,  C07D417/14 ,  C07D491/147 ,  C07H21/00 ,  C09B15/00 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2563/173

Abstract: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.

Abstract translation: 提供二聚和三聚体核酸染料以及相关系统和方法。 这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。 在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。 本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。