公开(公告)号：US08673570B2

公开(公告)日：2014-03-18

申请号：US13403012

申请日：2012-02-23

申请人： David A. Shafer

发明人： David A. Shafer

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C07H21/04 ,  C12Q1/68 ,  C12Q2521/107 ,  C12Q2525/155 ,  C12Q2525/161

摘要： The invention provides a series of reagent compositions and methods for making and amplifying novel cDNA based probe sets from RNA samples to improve analysis with gene expression arrays. The methods globally produce probe sets with common universal linkers at one or both ends, called WRAP-Probes, wherein the linkers do not bind to the target sequences and they can efficiently bind added reporters to the probes. The universal linkers are also designed as primer binding sites for copying and amplifying the probes, either linearly with one linker, or exponentially with double linkers. The capacity to globally and exponentially amplify the probe set by PCR is a primary advantage. Adding reporters by terminal linkers also improves quantification since each probe gets equivalent signaling. The invention allows expression analysis of small research, clinical and forensic samples to enable improved diagnostics, drug discovery, therapeutic monitoring, and medical, agricultural and general research.

摘要翻译： 本发明提供一系列用于从RNA样品制备和扩增新的基于cDNA的探针组的试剂组合物和方法，以改进基因表达阵列的分析。 这些方法全局产生探针组，其中一端或两端具有通用通用接头，称为WRAP探针，其中接头不与目标序列结合，并且它们可以有效地将添加的记录结合到探针中。 通用接头还被设计为用于复制和放大探针的引物结合位点，线性地与一个接头连接，或以双连接体指数地形成。 通过PCR全局和指数扩增探针组的能力是主要的优点。 由终端连接器添加记者也可以提高量化，因为每个探针都获得等效信号。 本发明允许小型研究，临床和法医样品的表达分析，以改进诊断，药物发现，治疗监测以及医学，农业和一般研究。

公开(公告)号：US20120058474A1

公开(公告)日：2012-03-08

申请号：US13294427

申请日：2011-11-11

申请人： David A. Shafer

发明人： David A. Shafer

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6818 ,  C12Q2537/162 ,  C12Q2525/173 ,  C12Q2525/161 ,  C12Q2565/525 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2537/161 ,  C12Q2537/1373

摘要： The invention provides novel compositions and methods for detecting unlabeled nucleic acid targets using labeled polynucleotide probes and partially complementary antiprobes. The interaction of probes, antiprobes and targets result in signaling changes that indicate target frequency. This novel detection mechanism is called a DNA detection switch, and it enable end-point detection, microarray detection and real-time PCR detection of a variety of nucleic acid targets including microbial species and subspecies, drug resistant mutants, and pathogenic strains.

摘要翻译： 本发明提供用于使用标记的多核苷酸探针和部分互补的抗体来检测未标记的核酸靶的新型组合物和方法。 探针，对手和靶标的相互作用导致指示目标频率的信号变化。 这种新型检测机制称为DNA检测开关，可实现多种核酸靶点（包括微生物物种和亚种，耐药性突变体和致病菌株）的终点检测，微阵列检测和实时PCR检测。