公开(公告)号：US20010055114A1

公开(公告)日：2001-12-27

申请号：US09764049

申请日：2001-01-19

发明人： Tomohiro Suzuki ,  Tadashi Okamoto ,  Kazuhiro Matsumoto ,  Nobuko Yamamoto

IPC分类号： G01N021/64 ,  G01J003/30

CPC分类号： G01N21/6458 ,  G01J3/4406 ,  G01N21/6428

摘要： To provide a method of efficiently removing excitation light in a device for measuring fluorescence emitted from samples on a measuring surface of a substrate while illuminating the samples with excitation light. The method is a fluorometry characterized in that an excitation light illumination portion where the samples are illuminated with the excitation light and a light detecting portion where measurements are made of the fluorescence are placed in such a manner as to make it possible to prevent the excitation light from approaching the light detecting portion, and measurements of the fluorescence emitted from the samples on the measuring surface of the substrate are made in such a manner as to relatively move the samples from the excitation light illumination portion to the light detecting portion after illuminating the same with the excitation light.

摘要翻译： 提供一种在用激发光照射样品的同时在基板的测量表面上测量从样品发射的荧光的装置中有效地去除激发光的方法。 该方法是一种荧光测定法，其特征在于，其中用激发光照射样品的激发光照射部分和其中测量荧光的光检测部分以使得可以防止激发光 从接近光检测部分，并且以这样的方式制作从基板的测量表面上的样品发射的荧光的测量，使得样品从激发光照射部分相对移动到光检测部分 用激发光。

公开(公告)号：US20010042837A1

公开(公告)日：2001-11-22

申请号：US09805315

申请日：2001-03-13

申请人： Leica Microsystems Heidelberg GmbH

发明人： Juergen Hoffmann

IPC分类号： G01N021/64

CPC分类号： G02B21/0056 ,  G02B21/0032 ,  G02B21/0068 ,  G02B21/0076

摘要： The present invention concerns an apparatus for illuminating a specimen (1), preferably in confocal fluorescence scanning microscopy, having one illumination beam path (2) of one light source (3) and at least one further illumination beam path (4) of a further light source (5), the illumination beam paths (2, 4) being at least partially superimposable on one another, and in order to simplify alignment and reduce the optical components in the illumination beam path, is characterized in that at least one optical component (7) is arranged at least in one of the illumination beam paths (2, 4) and modifies the light; and that the optical properties of the component (7) can be influenced or modified in such a way that the illumination pattern of the illumination beam path (2, 4) in the specimen region changes shape.

摘要翻译： 本发明涉及一种用于照射样本（1）的装置，优选地在共焦荧光扫描显微镜下，具有一个光源（3）的一个照明光束路径（2）和另外一个光源（3）的至少一个另外的照明光束路径（4） 光源（5），照明光束路径（2,4）彼此至少部分重叠，并且为了简化对准并减少照明光束路径中的光学部件，其特征在于至少一个光学部件 （7）至少布置在所述照明光束路径（2,4）中的一个中并修改所述光; 并且可以以这样的方式影响或修改组件（7）的光学特性，使得样本区域中的照明光束路径（2,4）的照明图案改变形状。

公开(公告)号：US20010021063A1

公开(公告)日：2001-09-13

申请号：US09769215

申请日：2001-01-24

申请人： Leica Microsystems Heidelberg GmbH

发明人： Werner Knebel

IPC分类号： G01N021/64 ,  G02B021/00 ,  G02B021/06 ,  G01T001/10 ,  G01J001/58

CPC分类号： G02B21/0032 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/082 ,  G02B21/18

摘要： A microscope, in particular for confocal scanning microscopy, having a light source (1) for illuminating an object (6) to be investigated. An optical device (9, 12) is provided for splitting transmitted light (15) passing through the object (6) and fluorescent light (10, 13) produced in the object (6). A segmenting device (17) acting on the transmitted light (15) is configured with regard to a detection-light path which is as short as possible in such a way that the segmenting device (17) is arranged between the object (6) and the light-splitting device (9, 12).

摘要翻译： 显微镜，特别是用于共聚焦扫描显微镜的显微镜，具有用于照亮被研究物体（6）的光源（1）。 提供了一种用于分离穿过物体（6）的透射光（15）和在物体（6）中产生的荧光（10,13）的光学装置（9,12）。 作用在透射光（15）上的分割装置（17）相对于尽可能短的检测光路被配置，使得分割装置（17）布置在物体（6）和 分光装置（9,12）。

公开(公告)号：US20010013930A1

公开(公告)日：2001-08-16

申请号：US09842513

申请日：2001-04-25

发明人： Mark Eyolfson ,  Elton J. Hochhalter ,  Joe Lee Phillips ,  David R. Johnson ,  Peter S. Frank

IPC分类号： G01N021/64 ,  G01N021/88

CPC分类号： G01N21/6456 ,  G01N21/4738 ,  G01N21/643 ,  G01N21/6458 ,  G01N21/94 ,  G01N21/9501 ,  G01N2021/6421 ,  G01N2021/646 ,  G01N2021/6471

摘要： A method and apparatus for detection of a particular material, such as photo-resist material, on a sample surface. A narrow beam of light is projected onto the sample surface and the fluoresced and/or reflected light intensity at a particular wavelength band is measured by a light detector. The light intensity is converted to a numerical value and transmitted electronically to a logic circuit which determines the proper disposition of the sample. The logic circuit controls a sample-handling robotic device which sequentially transfers samples to and from a stage for testing and subsequent disposition. The method is particularly useful for detecting photo-resist material on the surface of a semiconductor wafer.

摘要翻译： 用于在样品表面上检测诸如光致抗蚀剂材料的特定材料的方法和装置。 将窄光束投影到样品表面上，并且通过光检测器测量在特定波长带处的荧光和/或反射光强度。 将光强度转换为数值，并将其电子传输到确定样品的正确配置的逻辑电路。 逻辑电路控制一个样本处理机器人装置，该装置将样本顺序地传送到用于测试和随后的配置的阶段。 该方法对于在半导体晶片的表面上检测光致抗蚀剂材料特别有用。

公开(公告)号：US20010001642A1

公开(公告)日：2001-05-24

申请号：US09091907

申请日：1998-06-24

申请人： Ingo Klimant et al

发明人： INGO KLIMANT ,  GERHARD HOLST

IPC分类号： G01N021/64

CPC分类号： G01N21/7703 ,  G01K11/3213 ,  G01N21/6428 ,  G01N2021/772 ,  G01N2021/7786

摘要： The invention relates to a device for the temperature-compensated determination of chemical parameters, comprising: an optical temperature sensor which contains a temperature indicator that has a temperature-dependent afterglow time and/or luminous intensity and does not react with the surrounding medium; a chemical sensor which contains an indicator that is sensitive to a chemical parameter; means for stimulating the temperature indicator and the chemical indicator to luminesce; means for measuring the luminescence of the temperature indicator and of the chemical indicator; means for establishing an optical connection between indicator, excitation device and measuring equipment; means for detecting luminous radiation.

摘要翻译： 本发明涉及一种用于温度补偿确定化学参数的装置，包括：光学温度传感器，其包含具有温度依赖性余辉时间和/或发光强度并且不与周围介质反应的温度指示器; 包含对化学参数敏感的指示剂的化学传感器; 用于刺激温度指示器和化学指示剂发光的手段; 用于测量温度指示器和化学指示器的发光的装置; 用于在指示器，励磁装置和测量设备之间建立光学连接的装置; 用于检测发光辐射的装置。

公开(公告)号：US20040252304A1

公开(公告)日：2004-12-16

申请号：US10821727

申请日：2004-04-08

发明人： Philippe Jean Goix ,  Paul J. Lingane

IPC分类号： G01N021/64

CPC分类号： G01N15/147 ,  G01N15/1434 ,  G01N21/645 ,  G01N2021/6421

摘要： A multicolor particle analyzer and method is described. The particles each of which either naturally fluoresce or are tagged to fluoresce at distinctive wavelengths are caused to flow through an analyzing volume where fluorescence is excited by an impinging light beam. A tunable optical filter repetitively and sequentially passes emitted light at each of the characteristic wavelengths as each particle travels through the analyzing volume and the light transmitted through the optical filter is received by single detector which provides output signals representative of each distinct wavelength of light emitted by the particle.

摘要翻译： 描述了一种多色粒子分析仪和方法。 天然荧光或被标记以在特定波长发荧光的颗粒被引导流过分析体积，其中荧光被入射光束激发。 可调谐滤光器每个粒子通过分析体积重复地依次通过发射的每个特征波长的光，并且透过滤光器的光被单个检测器接收，该检测器提供表示由 颗粒。

公开(公告)号：US20040223152A1

公开(公告)日：2004-11-11

申请号：US10763681

申请日：2004-01-23

发明人： Salvatore Atzeni ,  James Mattheis ,  Raymond Kaminski

IPC分类号： G01N021/64

CPC分类号： G01N21/6458 ,  G01N21/6408

摘要： An optical imager, such as a microscope for performing multiple frequency fluorometric measurements comprising a light source, such as a laser source is disclosed. The system is used to excite a sample into the fluorescent state. Light from the excited sample is collected by a microscope. The microscope utilizes conventional confocal optics optimized to have a very narrow depth of field, thus limiting the information collected to a thin planar region. Measurements are taken over the fluorescence lifetime of the sample simultaneously from the excitation source and from the excited sample. Information is taken in a matrix and comparison of the image matrix and the standard during simultaneous measurements yields output information.

摘要翻译： 公开了一种光学成像仪，例如用于执行包括光源（例如激光源）的多频荧光测量的显微镜。 该系统用于激发样品进入荧光状态。 来自激发样品的光由显微镜收集。 该显微镜利用了经过优化的传统共焦光学镜头，具有非常窄的景深，从而将收集的信息限制在薄平面区域。 从激发源和激发的样品同时测量样品的荧光寿命。 以矩阵形式获取信息，同时测量时图像矩阵与标准的比较可以产生输出信息。

公开(公告)号：US20040223151A1

公开(公告)日：2004-11-11

申请号：US10868712

申请日：2004-06-15

发明人： Tsipouras Petros ,  Tafas Triantafyllos

IPC分类号： G01N021/64

CPC分类号： G02B1/045 ,  G01N21/7703 ,  G01N2021/1725 ,  G01N2021/7783 ,  G02B6/02033 ,  G02B6/1221

摘要： The present invention provides devices and methods for detection of particles, such as biological cells, in samples using a photosensitive waveguide. The photosensitive waveguide changes its transmissivity in a detectable manner in response to controlling radiation emitted from the particles. In preferred embodiments, the waveguide is two-dimensional and the position of the particles as well as their presence is obtained by scanning the waveguide in two non-parallel directions. The provided devices are preferably used to locate labeled cells. The present invention also includes control systems and methods for detecting and locating cells using the devices provided.

公开(公告)号：US20040222384A1

公开(公告)日：2004-11-11

申请号：US10863535

申请日：2004-06-08

发明人： Jerald D. Lee ,  Stanley D. Dabell

IPC分类号： G01N021/64

CPC分类号： G01N21/6452 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/1805 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2201/062 ,  Y10T436/143333

摘要： This invention concerns a fluorometer preferably combined with a thermal cycler useful in biochemical protocols such as polymerase chain reaction (PCR) and DNA melting curve analysis. The present fluorometer features a low heat-generating light source such as a light emitting diode (LED), having a one-to-one correspondence to each of a plurality of sample containers, such as capped PCR tubes in a standard titer tray. The fluorometer of the present invention further comprises an optical path between each LED and its correspondingly positioned container, and another optical path between each fluorescing sample within the positioned container and an optical signal sensing means. The instrument can be computer controlled.

摘要翻译： 本发明涉及一种荧光计，优选与可用于生物化学方案如PCR聚合酶链反应（PCR）和DNA熔解曲线分析的热循环仪组合。 本发明的荧光计具有诸如发光二极管（LED）的低发热光源，其与多个样品容器中的每一个具有一一对应关系，例如标准滴定盘中的封盖PCR管。 本发明的荧光计还包括在每个LED和其相应定位的容器之间的光路，以及在定位的容器内的每个荧光样品与光信号感测装置之间的另一光路。 仪器可以由电脑控制。

公开(公告)号：US20040218174A1

公开(公告)日：2004-11-04

申请号：US10833717

申请日：2004-04-27

申请人： Olympus Corporation

发明人： Yasuaki Natori

IPC分类号： G01N021/64

CPC分类号： G01N21/6458 ,  G01N21/39 ,  G01N21/6428 ,  G01N2021/6423 ,  G01N2021/6441 ,  G01N2201/067

摘要： A laser beam is scanned on a sample while a wavelength of the laser beam is varied. Quantity of fluorescence from the sample is detected. Absorption wavelength spectral characteristic data of fluorescence indicators whose kinds are known and which dye the sample is acquired. The absorption wavelength spectral characteristic data of a site to be discriminated is compared with the absorption wavelength spectral characteristic data of each of the fluorescence indicators of known kinds. The kind of fluorescence indicator dyeing the site to be discriminated is discriminated from a result of the comparison.

摘要翻译： 在激光束的波长变化的同时，在样品上扫描激光束。 检测来自样品的荧光量。 荧光指示器的吸收波长光谱特征数据，其种类是已知的，并且获取样品的染料。 将要鉴别的部位的吸收波长光谱特征数据与已知种类的每个荧光指示剂的吸收波长光谱特征数据进行比较。 对待鉴定场所的荧光指示剂的种类与比较的结果进行区分。