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1918 条记录 (耗时 0.038 秒)

    Method of screening, isolating, and purifying rare cells
    111.
    发明授权
    Method of screening, isolating, and purifying rare cells 有权

    公开(公告)号：US10578607B2

    公开(公告)日：2020-03-03

    申请号：US15817431

    申请日：2017-11-20

    申请人： Min-Hsien Wu ,  Wen-Pin Chou ,  Tzu-Keng Chiu ,  Po-Yu Chu

    发明人： Min-Hsien Wu ,  Wen-Pin Chou ,  Tzu-Keng Chiu ,  Po-Yu Chu

    IPC分类号： G01N33/49 ,  C12N5/09 ,  G01N27/327 ,  G01N33/574 ,  G01N27/447 ,  C12N13/00

    摘要： A method of screening, isolating and purifying rare cells includes obtaining cell groups with rare cells from a sample, removing cells not to be screened from the cell groups, and drawing the remaining cells as a cell suspension sample. An introduction device is activated to load the cell suspension sample onto an optically-induced dielectrophoresis chip including a cell isolation zone having a division arrangement wherein at least one optical pattern is configured on the cell isolation zone for screening, isolating and purifying the rare cells. The at least one optical pattern is configured to migrate, rotate or both. The at least one optical pattern is activated to attract the rare cells into a collection tank on the chip, and a liquid drawing device is activated to draw the rare cells out of the collection tank, thereby obtaining purified rare cells from the sample.

    Cell mass culture vessel
    116.
    发明授权
    Cell mass culture vessel 有权

    公开(公告)号：US10472598B2

    公开(公告)日：2019-11-12

    申请号：US15310224

    申请日：2015-05-20

    申请人： SUMITOMO BAKELITE CO., LTD.

    发明人： Ryohei Tsukada ,  Haruo Okubo

    IPC分类号： B01L3/00 ,  C12M1/00 ,  C12M1/32 ,  C12N1/00 ,  C12N5/09

    摘要： A cell aggregate culture vessel (1) is a vessel for culturing a cell aggregate. The cell aggregate culture vessel (1) includes a well (21) having a culture space capable of storing the cell aggregate and culture fluid and a tubular body (3) which is disposed on the well on a plane at which the well has an opening and have an inner cavity communicating with the culture space, and one or more communication portion (3a) capable of discharging the culture fluid without allowing passage of the cell aggregate to the outside of the tubular body (3) is formed in a tubular wall of the tubular body (3). The communication portion (3a) may be, for example, a slit having a width of 0.1 mm or larger and 0.5 mm or smaller.

    Particle filtering device and method
    117.
    发明授权
    Particle filtering device and method 有权

    公开(公告)号：US10465168B2

    公开(公告)日：2019-11-05

    申请号：US14780002

    申请日：2015-04-30

    申请人： UNIST ACADEMY-INDUSTRY RESEARCH CORPORATION

    发明人： Yoon-Kyoung Cho ,  Tae-Hyeong Kim

    IPC分类号： C12N5/09 ,  C12M1/00 ,  C12M1/26

    摘要： A particle filtering device which shortens the separation time of particles and increases the separation efficiency are provided. The particle filtering device comprises: a filtration membrane which separates a particle by filtering a sample; a first body connected to an inlet side of the filtration membrane, which supplies the sample to the inlet side of the filtration membrane; and a second body connected to an outlet side of the filtration membrane, which accommodates a permeate whose particle is separated through the filtration membrane, wherein the particle filtering device has a structure in which the permeate is accommodated in advance between the second body and the outlet side of the filtration membrane.

    METHOD AND KIT FOR EXPANDING CIRCULATING TUMOR CELLS IN VITRO
    120.
    发明申请
    METHOD AND KIT FOR EXPANDING CIRCULATING TUMOR CELLS IN VITRO 审中-公开

    公开(公告)号：US20190300832A1

    公开(公告)日：2019-10-03

    申请号：US16029680

    申请日：2018-07-09

    申请人： Taipei Medical University

    发明人： Peng-Yuan Wang ,  Yin-Ju Chen ,  Long-Sheng Lu ,  Thierry Burnouf

    IPC分类号： C12M1/12 ,  C12N5/09 ,  G01N33/50

    摘要： A method for expanding circulating tumor cell in vitro includes preparing a cell culture tool having a multi-particle colloidal crystal layer, preparing a cell solution including circulating tumor cells, and contacting the cell solution with the multi-particle colloidal crystal layer, to attach the circulating tumor cells in the cell solution to the multi-particle colloidal crystal layer and rapidly expand by 20 times or more. The multi-particle colloidal crystal layer at least includes first particles having a particle size of 1000 to 5000 nm and second particles having a particle size of 20 to 400 nm. The culture medium in the cell solution at least includes a platelet lysate.