公开(公告)号：US10128097B2

公开(公告)日：2018-11-13

申请号：US15606318

申请日：2017-05-26

申请人： Micromass UK Limited

发明人： Michael Raymond Morris ,  Steven Derek Pringle ,  Keith Richardson ,  Ian Sinclair

IPC分类号： H01J49/10 ,  H01J49/04 ,  G01N29/02 ,  H01J49/00

摘要： An ion source for a mass spectrometer is disclosed comprising an ultrasonic transducer which focuses ultrasonic energy onto a surface of a sample fluid without directly contacting the sample fluid.

公开(公告)号：US10083824B2

公开(公告)日：2018-09-25

申请号：US15317045

申请日：2015-06-11

申请人： Micromass UK Limited

发明人： Kevin Giles ,  Martin Raymond Green ,  Steven Derek Pringle ,  Keith Richardson ,  Jason Lee Wildgoose

IPC分类号： H01J49/00 ,  G01N27/62

CPC分类号： H01J49/0031 ,  G01N27/622 ,  H01J49/0045

摘要： A method of analyzing ions, comprising performing an initial multidimensional survey scan comprising separating parent ions according to a first physico-chemical property and separating said parent ions according to a second physico-chemical property, producing a two-dimensional data set comprising data corresponding to said first physico-chemical property and data corresponding to said second physico-chemical property, identifying one or more target ion species of interest and determining a mode of operation of a mass spectrometer for said target ion species of interest using data relating to said target ion species of interest in said two-dimensional data set, wherein said mode of operation comprises the location of fragmentation of said target ions of interest.

公开(公告)号：US20180174817A1

公开(公告)日：2018-06-21

申请号：US15578053

申请日：2016-05-31

申请人： Micromass UK Limited

发明人： Martin Green ,  Jason Wildgoose ,  Keith Richardson ,  Kevin Giles ,  Daniel Kenny ,  David Langridge ,  Richard Moulds

IPC分类号： H01J49/42 ,  H01J49/06

CPC分类号： H01J49/4215 ,  H01J49/063 ,  H01J49/067 ,  H01J49/4255

摘要： A mass filter is disclosed having at least one electrode (42-48) comprising an aperture (43) or recess. Voltages are applied to the electrodes (42-48) of the mass filter such that ions having mass to charge ratios in a desired range are confined by the electrodes and are transmitted along and through the mass filter, whereas ions (47,49) having mass to charge ratios outside of said desired range are unstable and pass into the aperture (43) or recess such that they are filtered out by the mass filter. The aperture (43) or recess reduces or eliminates the number of ions that would otherwise impact the electrode surface facing the ion transmission axis and hence reduces degradation of the ion transmission properties of the mass filter.

公开(公告)号：US20170254776A1

公开(公告)日：2017-09-07

申请号：US15125406

申请日：2015-03-10

申请人： Micromass UK Limited

发明人： Martin Raymond Green ,  Kevin Giles ,  Keith Richardson ,  Jason Lee Wildgoose

IPC分类号： G01N27/62 ,  H01J49/00

CPC分类号： H01J49/0036 ,  G01N27/622 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/005

摘要： A method of mass spectrometry is disclosed comprising calculating an ion mobility value, collision cross section or interaction cross section of a plurality of different analyte ions under one or more different analytical conditions, and setting one or more operational parameters of a mass spectrometer in response to the calculated ion mobility values, collision cross sections or the calculated interaction cross sections so as to maximise or enhance a subsequent ion mobility separation of a plurality of different analyte ions.

公开(公告)号：US09697996B2

公开(公告)日：2017-07-04

申请号：US14774756

申请日：2014-03-10

申请人： Micromass UK Limited

发明人： Jeffery Mark Brown ,  Kevin Giles ,  Martin Raymond Green ,  John Brian Hoyes ,  Christopher Jones ,  Michael Raymond Morris ,  Steven Derek Pringle ,  Keith Richardson ,  Farnoush Salarzaei ,  Jason Lee Wildgoose

IPC分类号： H01J49/00 ,  G01N27/62

CPC分类号： H01J49/005 ,  G01N27/622 ,  H01J49/0031 ,  H01J49/0045 ,  H01J49/0072

摘要： A method of mass spectrometry is disclosed comprising: performing a survey scan of a plurality of different types of parent or precursor ions, wherein the survey scan comprises analyzing the ion mobilities of the ions and mass analyzing the ions; determining the charge states of parent or precursor ions analyzed in the survey scan based on their determined combinations of ion mobility and mass to charge ratio; selecting a parent or precursor ion for fragmentation or reaction; and fragmenting or reacting the selected ion, wherein the fragmentation or reaction conditions are selected from a plurality of different fragmentation or reaction conditions based upon the determined charge state of the selected ion.

公开(公告)号：US09552970B2

公开(公告)日：2017-01-24

申请号：US14772448

申请日：2014-03-05

申请人： Micromass UK Limited

发明人： Kevin Giles ,  David J. Langridge ,  Keith Richardson ,  Jason Lee Wildgoose

IPC分类号： H01J49/40 ,  H01J49/00 ,  G01N27/62

CPC分类号： H01J49/0031 ,  G01N27/622 ,  H01J49/401

摘要： A method of analyzing ions is disclosed comprising: (i) separating ions according to a physico-chemical property in a separator; (ii) transmitting ions which emerge from the separator through a transfer device with a first transit time t1, energizing a pusher electrode or orthogonal acceleration electrode and obtaining first data; (iii) transmitting ions which subsequently emerge from the separator through the transfer device with a second greater transit time t2, energizing the pusher electrode or orthogonal acceleration electrode and obtaining second data; and (iv) repeating steps (ii) and (iii) one or more times. The pusher electrode or orthogonal acceleration electrode is energized with a period t3, wherein t2-t1 is arranged to equal t3/2. The first and second data are combined to form a composite data set.

摘要翻译： 公开了分析离子的方法，包括：（i）根据分离器中的物理化学性质分离离子; （ii）通过具有第一传送时间t1的传送装置传送从分离器出射的离子，激励推动器电极或正交加速电极并获得第一数据; （iii）传输随后通过传送装置从分离器中排出的离子，其具有第二较大传播时间t2，激励推进器电极或正交加速电极并获得第二数据; 和（iv）重复步骤（ii）和（iii）一次或多次。 推动器电极或正交加速电极以周期t3通电，其中t2-t1被设置为等于t3 / 2。 第一和第二数据被组合以形成复合数据集。

公开(公告)号：US20170003250A1

公开(公告)日：2017-01-05

申请号：US15100034

申请日：2014-12-01

申请人： MICROMASS UK LIMITED

发明人： Martin Raymond Green ,  Keith Richardson ,  Jason Lee Wildgoose

IPC分类号： G01N27/447 ,  H01J49/04 ,  G01N27/62 ,  H01J49/00

CPC分类号： G01N27/44717 ,  G01N27/4473 ,  G01N27/44782 ,  G01N27/44791 ,  G01N27/624 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/0404

摘要： A method of mass spectrometry or ion mobility spectrometry is disclosed in which analyte ions of a desired charge state are isolated. The method comprises: separating analytes according to their electrophoretic mobility; ionising the analytes; and mass filtering the resulting analyte ions, wherein the mass to charge ratios of the ions transmitted by a mass filter are varied as a function of the electrophoretic mobility and according to a predetermined relationship such that substantially only ions having said desired charge state are transmitted by the mass filter.

摘要翻译： 公开了一种质谱法或离子迁移光谱法，其中分离出所需电荷状态的分析物离子。 该方法包括：根据其电泳迁移率分离分析物; 电离分析物; 并且对所得到的分析离子进行质量过滤，其中质量过滤器传输的离子的质荷比随着电泳迁移率的变化而变化，并且根据预定关系使得基本上仅具有所需电荷状态的离子通过 质量过滤器。

公开(公告)号：US09484192B2

公开(公告)日：2016-11-01

申请号：US14776036

申请日：2014-03-14

申请人： Micromass UK Limited

发明人： Richard Chapman ,  Jeffery Mark Brown ,  Paul Murray ,  Keith Richardson ,  Emmy Maria Hoyes

IPC分类号： H01J49/00

CPC分类号： H01J49/0004 ,  H01J49/0031 ,  H01J49/0036

摘要： A method of ion imaging is disclosed comprising scanning a sample and acquiring first mass spectral data related to a first pixel location at a first spatial resolution and determining whether or not the first mass spectral data satisfies a condition. If it is determined that the first mass spectral data does satisfy the condition then the first mass spectral data is stored, recorded or prioritized. If it is determined that the first mass spectral data does not satisfy the condition then the first mass spectral data is discarded or downgraded. Scanning of the sample then continues at the first spatial resolution and further mass spectral data related to further pixel locations is acquired.

摘要翻译： 公开了一种离子成像方法，包括扫描样品并以第一空间分辨率获取与第一像素位置相关的第一质谱数据，并确定第一质谱数据是否满足条件。 如果确定第一质谱数据确实满足条件，则存储，记录或优先化第一质谱数据。 如果确定第一质谱数据不满足条件，则第一质谱数据被丢弃或降级。 样本的扫描然后以第一空间分辨率继续进行，并且获取与另外的像素位置相关的进一步的质谱数据。

公开(公告)号：US20160254129A1

公开(公告)日：2016-09-01

申请号：US15035935

申请日：2014-11-11

申请人： Micromass UK Limited

发明人： Keith Richardson ,  Jason Lee Wildgoose

IPC分类号： H01J49/00

CPC分类号： H01J49/0036 ,  G01N15/1031 ,  G01N27/622 ,  G01N2015/0003 ,  G01N2015/1043 ,  H01J49/0031 ,  H01J49/0045

摘要： A method of mass spectrometry is disclosed comprising comparing mass spectral data so as to identify a precursor ion in the mass spectral data that has a predetermined mass difference to a product ion in the mass spectral data; and determining whether said precursor ion is a precursor ion of interest by comparing the ion signal profile for the precursor ion with the ion signal profile for the product ion. If the profiles match then the precursor ion is determined to be an ion of interest. When a precursor ion is determined to be an ion of interest, the precursor ion is isolated from other precursor ions, fragmented or reacted so as to produce product ions, and the product ions are analysed so as to obtain product ion data that can be used to identify the precursor ion.

摘要翻译： 公开了一种质谱方法，其包括比较质谱数据，以便在质谱数据中识别与产物离子具有预定质量差异的质谱数据中的前体离子; 以及通过将前体离子的离子信号分布与产物离子的离子信号分布进行比较来确定所述前体离子是否是所关注的前体离子。 如果轮廓匹配，则前体离子被确定为感兴趣的离子。 当前体离子被确定为感兴趣的离子时，前体离子与其它前体离子分离，碎裂或反应以产生产物离子，并分析产物离子，以获得可以使用的产物离子数据 以鉴定前体离子。

公开(公告)号：US09390896B2

公开(公告)日：2016-07-12

申请号：US14776175

申请日：2014-03-14

申请人： Micromass UK Limited

发明人： Jeffery Mark Brown ,  Richard Chapman ,  Paul Murray ,  Keith Richardson ,  Emmy Maria Hoyes

IPC分类号： H01J49/00 ,  H01J49/16

CPC分类号： H01J49/0004 ,  H01J49/164

摘要： A method of ion imaging is disclosed comprising scanning a sample at a first resolution and acquiring first mass spectral data related to a first pixel location. A determination is then made as to whether or not the first mass spectral data satisfies a condition, wherein if it is determined that the first mass spectral data does satisfy the condition then the method further comprises: (i) switching to acquire second mass spectral data related to a second pixel location which is substantially adjacent to the first pixel location so that the second mass spectral data is acquired at a second resolution which is higher than the first resolution; and (ii) determining whether or not the second mass spectral data satisfies the condition, wherein if it is determined that the second mass spectral data does satisfy the condition then the method further comprises acquiring third mass spectral data related to a third pixel location which is substantially adjacent to the first or second pixel locations so that the third mass spectral is acquired at the second resolution and wherein if it is determined that the second or third mass spectral data does not satisfy the condition then the method further comprises switching back to scanning the sample at the first resolution.

摘要翻译： 公开了一种离子成像方法，包括以第一分辨率扫描样品并获取与第一像素位置相关的第一质谱数据。 然后确定第一质谱数据是否满足条件，其中如果确定第一质谱数据确实满足条件，则该方法还包括：（i）切换以获取第二质谱数据 涉及与第一像素位置基本相邻的第二像素位置，使得以高于第一分辨率的第二分辨率获取第二质谱数据; 以及（ii）确定所述第二质谱数据是否满足所述条件，其中如果确定所述第二质谱数据确实满足所述条件，则所述方法还包括获取与第三像素位置有关的第三质谱数据，所述第三质谱数据是 基本上与第一或第二像素位置相邻，使得以第二分辨率获取第三质谱，并且其中如果确定第二或第三质谱数据不满足条件，则该方法还包括切换回扫描 以第一分辨率进行抽样。