公开(公告)号：US20060192100A1

公开(公告)日：2006-08-31

申请号：US11349551

申请日：2006-02-07

Applicant: Roman Zubarev ,  Alexander Misharin ,  Oleg Silivra ,  Frank Kjeldsen

Inventor： Roman Zubarev ,  Alexander Misharin ,  Oleg Silivra ,  Frank Kjeldsen

IPC: H01J49/00 ,  B01D59/44

CPC classification number: H01J49/005 ,  G01N33/6848 ,  H01J49/02 ,  Y10S250/91 ,  Y10T436/17

Abstract: The invention relates to a method and apparatus for the fragmentation of large molecules, especially biopolymers. The invention consists in reacting analyte ions with excited or radical neutral particles, whereby, at least in the case of bombardment of analyte ions with helium atoms from an FAB generator, a new type of fragmentation occurs which strongly resembles fragmentation by electron capture (ECD). The reactions may be performed in magnetic ion traps (ion cyclotron resonance cells, ICR), in RF ion traps according to Wolfgang Paul, in RF ion guides, or in free beams of analyte ions or neutral particles.

公开(公告)号：US07655903B2

公开(公告)日：2010-02-02

申请号：US11737300

申请日：2007-04-19

Applicant: Roman Zubarev ,  Alexander Misharin

Inventor： Roman Zubarev ,  Alexander Misharin

IPC: H01J49/36

CPC classification number: H01J49/38

Abstract: An ion cyclotron resonance cell has at least one trapping electrode comprised of electrically isolated sections that are used for the detection of an induced ion image signal. Such an arrangement increases the sensitivity of image signal detection without a significant increase in the amplitude of parasitic harmonics. When a multielectrode detection arrangement is used, the resolving power of an analyzer incorporating such a cyclotron resonance cell multiplies without a corresponding sensitivity loss.

Abstract translation: 离子回旋共振池具有至少一个由用于检测诱导离子图像信号的电隔离部分组成的俘获电极。 这种布置增加了图像信号检测的灵敏度，而不会明显增加寄生谐波的幅度。 当使用多电极检测装置时，包含这种回旋共振元件的分析仪的分辨率倍增而没有相应的灵敏度损失。

公开(公告)号：US08304715B2

公开(公告)日：2012-11-06

申请号：US12756015

申请日：2010-04-07

Applicant: Alexander Misharin ,  Roman Zubarev

Inventor： Alexander Misharin ,  Roman Zubarev

IPC: H01J49/00 ,  B01D59/44

CPC classification number: H01J49/38

Abstract: A measuring cell of an ICR mass spectrometer and a method of operating a measuring cell of the ICR mass spectrometer. The method and system trap ions in a first compartment of the ICR measuring cell by generating an electric potential well in the direction of the magnetic field with a minimum of the electric potential well located inside the first compartment. The method and system excite cyclotron motion of the ions trapped in the first compartment. The method and system transfer at least a part of the excited ions from the first compartment to a second compartment of the ICR measuring cell by displacement of a position of the minimum of the electric potential well from the first compartment to the second compartment. The ions are transferred by displacing the position of the minimum of the electric potential well from the first compartment to the second compartment preferably over a period of time equal to or longer than a characteristic period of ion oscillations along the direction of the magnetic field in the electric potential well. The method and system detect ion cyclotron motion of at least a part of the ions in the second compartment.

Abstract translation: ICR质谱仪的测量单元和操作ICR质谱仪的测量单元的方法。 该方法和系统通过在第一隔室内位于最小的电位下在磁场的方向上产生电位而在ICR测量池的第一隔室中捕获离子。 该方法和系统激发捕获在第一隔室中的离子的回旋加速器运动。 该方法和系统通过将最小电势井的位置从第一隔室移位到第二隔室，将激发的离子的至少一部分从第一隔室转移到ICR测量池的第二隔室。 通过将电位阱的最小值的位置从第一隔室移动到第二隔室，优选地在等于或长于沿着沿着磁场方向的离子振荡的特征周期的时间段 电势很好 该方法和系统检测第二隔室中至少一部分离子的离子回旋加速器运动。

公开(公告)号：US20110248159A1

公开(公告)日：2011-10-13

申请号：US12756015

申请日：2010-04-07

Applicant: Alexander MISHARIN ,  Roman Zubarev

Inventor： Alexander MISHARIN ,  Roman Zubarev

IPC: H01J49/34 ,  H01J49/02

CPC classification number: H01J49/38

Abstract: A measuring cell of an ICR mass spectrometer and a method of operating a measuring cell of the ICR mass spectrometer. The method and system trap ions in a first compartment of the ICR measuring cell by generating an electric potential well in the direction of the magnetic field with a minimum of the electric potential well located inside the first compartment. The method and system excite cyclotron motion of the ions trapped in the first compartment. The method and system transfer at least a part of the excited ions from the first compartment to a second compartment of the ICR measuring cell by displacement of a position of the minimum of the electric potential well from the first compartment to the second compartment. The ions are transferred by displacing the position of the minimum of the electric potential well from the first compartment to the second compartment preferably over a period of time equal to or longer than a characteristic period of ion oscillations along the direction of the magnetic field in the electric potential well. The method and system detect ion cyclotron motion of at least a part of the ions in the second compartment.

Abstract translation: ICR质谱仪的测量单元和操作ICR质谱仪的测量单元的方法。 该方法和系统通过在第一隔室内位于最小的电位下在磁场的方向上产生电位而在ICR测量池的第一隔室中捕获离子。 该方法和系统激发捕获在第一隔室中的离子的回旋加速器运动。 该方法和系统通过将最小电势井的位置从第一隔室移位到第二隔室，将激发的离子的至少一部分从第一隔室转移到ICR测量池的第二隔室。 通过将电位阱的最小值的位置从第一隔室移动到第二隔室，优选地在等于或长于沿着沿着磁场方向的离子振荡的特征周期的时间段 电势很好 该方法和系统检测第二隔室中至少一部分离子的离子回旋加速器运动。

公开(公告)号：US07476853B2

公开(公告)日：2009-01-13

申请号：US11349551

申请日：2006-02-07

Applicant: Roman Zubarev ,  Alexander Misharin ,  Oleg Silivra ,  Frank Kjeldsen

Inventor： Roman Zubarev ,  Alexander Misharin ,  Oleg Silivra ,  Frank Kjeldsen

IPC: B01D59/44 ,  H01J49/00

CPC classification number: H01J49/005 ,  G01N33/6848 ,  H01J49/02 ,  Y10S250/91 ,  Y10T436/17

Abstract: The invention relates to a method and apparatus for the fragmentation of large molecules, especially biopolymers. The invention consists in reacting analyte ions with excited or radical neutral particles, whereby, at least in the case of bombardment of analyte ions with helium atoms from an FAB generator, a new type of fragmentation occurs which strongly resembles fragmentation by electron capture (ECD). The reactions may be performed in magnetic ion traps (ion cyclotron resonance cells, ICR), in RF ion traps according to Wolfgang Paul, in RF ion guides, or in free beams of analyte ions or neutral particles.

Abstract translation: 本发明涉及一种用于大分子，特别是生物聚合物破碎的方法和装置。 本发明包括使分析物离子与激发或自由基中性颗粒反应，由此，至少在从FAB发生器轰击具有氦原子的分析物离子的情况下，发生类似于通过电子捕获（ECD）破碎的新型碎裂， 。 反应可以在磁离子阱（离子回旋共振池，ICR），RF离子阱中根据Wolfgang Paul，RF离子导向器或分析离子或中性粒子的自由光束进行。

公开(公告)号：US20070278402A1

公开(公告)日：2007-12-06

申请号：US11737300

申请日：2007-04-19

Applicant: Roman Zubarev ,  Alexander Misharin

Inventor： Roman Zubarev ,  Alexander Misharin

IPC: B01D59/44

CPC classification number: H01J49/38

Abstract: An ion cyclotron resonance cell has at least one trapping electrode comprised of electrically isolated sections that are used for the detection of an induced ion image signal. Such an arrangement increases the sensitivity of image signal detection without a significant increase in the amplitude of parasitic harmonics. When a multielectrode detection arrangement is used, the resolving power of an analyzer incorporating such a cyclotron resonance cell multiplies without a corresponding sensitivity loss.

Abstract translation: 离子回旋共振池具有至少一个由用于检测诱导离子图像信号的电隔离部分组成的俘获电极。 这种布置增加了图像信号检测的灵敏度，而不会明显增加寄生谐波的幅度。 当使用多电极检测装置时，包含这种回旋共振元件的分析仪的分辨率倍增而没有相应的灵敏度损失。

公开(公告)号：US20110121172A1

公开(公告)日：2011-05-26

申请号：US12992839

申请日：2009-05-04

Applicant: Mikhail M. Savitski ,  Roman Zubarev

Inventor： Mikhail M. Savitski ,  Roman Zubarev

IPC: B01D59/44 ,  H01J49/26

CPC classification number: H01J49/0031 ,  G06F19/703 ,  H01J49/0045

Abstract: A method of identifying precursor ion species from their fragments comprises obtaining mass spectra of a plurality of precursor ion species and their fragments to high mass accuracy. The fragment mass spectrum, obtained from fragmentation of multiple precursor ion species, is then scanned it identify pairs of fragments whose combined mass matches the mass of one of the precursor ion species. Once pairs of fragment ion shave been matched to precursor ions, the composite fragment ion spectrum is broken down into portions, one per fragment pair. Analysis continues until no further pairs are identified. A simplified fragment ion spectrum is then reconstructed for each precursor sample ion by stitching together the broken down sections of the composite fragment spectrum. The resultant reconstructed, simplified fragment spectra are sent to a search engine which returns a score-sorted list of likely candidates for each synthetic fragment ion spectrum.

Abstract translation: 从其片段鉴定前体离子种类的方法包括以高质量精度获得多种前体离子种类及其片段的质谱。 然后扫描从多个前体离子物质的断裂获得的片段质谱，鉴定其组合质量与一种前体离子物质的质量相匹配的片段对。 一对碎片离子与前体离子相匹配，复合片段离子谱被分解成部分，每个片段对一个。 分析继续进行，直到没有进一步的对被识别为止。 然后通过将复合片段谱的分解部分拼接在一起，为每个前体样品离子重建简化的片段离子谱。 所得到的重构的，简化的片段谱被发送到搜索引擎，该搜索引擎返回每个合成片段离子谱的可能候选的得分分类列表。

公开(公告)号：US20060255259A1

公开(公告)日：2006-11-16

申请号：US11403555

申请日：2006-04-13

Applicant: Roman Zubarev ,  Mikhail Savitski ,  Michael Nielsen

Inventor： Roman Zubarev ,  Mikhail Savitski ,  Michael Nielsen

IPC: B01D59/44

CPC classification number: H01J49/0045

Abstract: The invention relates to acquisition methods for fragment ion spectra of biopolymer molecules in tandem mass spectrometers which are coupled to separation devices. The invention provides a real-time method for calculating a quality coefficient for each fragment ion spectrum. The quality coefficient indicates whether the fragment ion spectrum can be used successfully for identifying the biopolymer molecule or whether it should be acquired once more, possibly with other acquisition parameters.

Abstract translation: 本发明涉及串联质谱仪中分离装置的生物聚合物分子的片段离子光谱的采集方法。 本发明提供了一种用于计算每个碎片离子光谱质量系数的实时方法。 质量系数表示碎片离子谱是否可以成功应用于识别生物聚合物分子，或者是否应该再次获得，可能还有其他采集参数。

公开(公告)号：US06958472B2

公开(公告)日：2005-10-25

申请号：US10471454

申请日：2002-03-22

Applicant: Roman Zubarev

Inventor： Roman Zubarev

IPC: H01J49/10 ,  H01J49/42

CPC classification number: H01J49/0054

Abstract: Methods and apparatus are provided to obtain efficient Electron capture dissociation (ECD) of positive ions, particularly useful in the mass spectrometric analysis of complex samples such as of complex mixtures and large biomolecules of peptides and proteins. Due to the low efficiency of ECD as previously used, the technique has so far only been employed with Penning cell ion cyclotron resonance mass spectrometers, where the ions are confined by a combination of magnetic and electrostatic fields. To substantially increase the efficiency of electron capture, the invention makes use of a high-intensity electron source producing a high-flux low-energy electron beam of a diameter comparable to that of the confinement volume of ions. Such a beam possesses trapping properties for positive ions. The ions confined by electron beam effectively capture electrons, which leads much shorter analysis time. The invention provides the possibility to employs ECD in other trapping and non-trapping instruments beside ICR mass spectrometers.

Abstract translation: 提供了方法和装置以获得正离子的有效电子捕获解离（ECD），特别可用于复杂样品如复合物混合物和大量生物分子的肽和蛋白质的质谱分析。 由于以前使用的ECD的效率低，该技术迄今仅用于Penning细胞离子回旋共振质谱仪，其中离子被磁场和静电场的组合限制。 为了显着提高电子俘获的效率，本发明利用高强度电子源产生直径与离子的约束体积相当的直径的高通量低能电子束。 这样的光束具有正离子的俘获特性。 由电子束限制的离子有效捕获电子，这导致更短的分析时间。 本发明提供了在ICR质谱仪旁边的其他捕获和非捕获仪器中采用ECD的可能性。

公开(公告)号：US06800851B1

公开(公告)日：2004-10-05

申请号：US10644648

申请日：2003-08-20

Applicant: Roman Zubarev ,  Frank Kjeldsen ,  Igor Ivonin ,  Oleg Silivra

Inventor： Roman Zubarev ,  Frank Kjeldsen ,  Igor Ivonin ,  Oleg Silivra

IPC: H01J3934

CPC classification number: H01J49/0054 ,  H01J49/063 ,  H01J49/4225

Abstract: The present invention relates to ion fragmentation techniques by electron-ion reactions in multipolar radiofrequency fields like those in quadrupole ion traps or in ion guides, and devices to perform ion fragmentation by such techniques. The fragmentation techniques are useful for tandem mass spectrometry. The invention consists of the application of a magnetic field essentially parallel to the axis of the radiofrequency field to confine the electrons in the direction perpendicular to the magnetic field.

Abstract translation: 本发明涉及通过多极射频场中的电子 - 离子反应的离子碎片化技术，如四极离子阱或离子导向器中的离子碎片化技术，以及通过这些技术进行离子碎裂的装置。 碎裂技术可用于串联质谱法。 本发明包括施加基本上平行于射频场的轴的磁场，以在垂直于磁场的方向上限制电子。