公开(公告)号：US20230294086A1

公开(公告)日：2023-09-21

申请号：US18016753

申请日：2021-07-29

Applicant: Colorado State University Research Foundation

Inventor： Charles S. Henry ,  Brian J. Geiss ,  David S. Dandy ,  Cody Carrell ,  Jeremy Link ,  Isabelle Samper ,  Ana Sanchez-Cano ,  Ilhoon Jang ,  Zachary Call

IPC: B01L3/00 ,  G01N33/543

CPC classification number: B01L3/5023 ,  G01N33/54388 ,  B01L3/50273 ,  B01L2200/16 ,  B01L2400/0406 ,  B01L2300/069 ,  B01L2300/088

Abstract: An assay device includes a colorimetric testing assembly including a detection area, a fluid inlet, and a microfluidic network including a first path extending to the detection area and a second path extending to the detection area. When a fluid (e.g., a buffer fluid or a combined buffer and sample solution) is provided to the fluid inlet, a first portion of the fluid rehydrates a first dried reagent (e.g., a dried enzyme label) disposed along the first path to produce a first rehydrated reagent and a second portion of the fluid rehydrates a second dried reagent (e.g., a dried substrate) to produce a second rehydrated reagent. The first rehydrated reagent and the second rehydrated reagent are then sequentially delivered to the detection area by capillary-driven flow to perform the assay.