公开(公告)号：US08211446B2

公开(公告)日：2012-07-03

申请号：US12870952

申请日：2010-08-30

Applicant: Pappachan E. Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

Inventor： Pappachan E. Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

IPC: A61K39/04 ,  A61K39/02 ,  C12Q1/02

CPC classification number: C12N1/36 ,  C12N1/20

Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.

Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。

公开(公告)号：US20090023596A1

公开(公告)日：2009-01-22

申请号：US12107146

申请日：2008-04-22

Applicant: Pappachan Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

Inventor： Pappachan Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

IPC: C40B30/04 ,  C40B40/02 ,  C40B50/06 ,  C12N1/20

CPC classification number: C12N1/36 ,  C12N1/20

Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.

Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。

公开(公告)号：US20110190479A1

公开(公告)日：2011-08-04

申请号：US12983704

申请日：2011-01-03

Applicant: PAPPACHAN E. KOLATTUKUDY ,  TATIANA SIRAKOVA ,  VINOD S. DUBEY ,  JAIYANTH DANIEL

Inventor： PAPPACHAN E. KOLATTUKUDY ,  TATIANA SIRAKOVA ,  VINOD S. DUBEY ,  JAIYANTH DANIEL

IPC: C07K16/12 ,  C12Q1/48 ,  C07H21/04

CPC classification number: C07H21/04 ,  C07K16/12 ,  C12Q1/48

Abstract: The application reports the discovery that mycobacterial triacylglycerol synthase (MTTGS) enzymes also have a wax synthase function. This bifunctional enzymatic activity, or ‘dual function,’ implies that the enzymes contain separate binding sites for diacylglycerol and acyl alcohol and a common binding site for acyl-coenzyme A. Embodiments of the invention relate to methods of selection and/or design of therapeutic compounds that will reduce any potentially toxic side effects of candidate drugs designed to inhibit the mycobacterial enzyme.

Abstract translation: 该应用报告了分枝杆菌三酰基甘油合酶（MTTGS）酶也具有蜡合酶功能的发现。 这种双功能酶活性或“双重功能”意味着酶含有二酰基甘油和酰基醇的单独结合位点和酰基辅酶A的常见结合位点。本发明的实施方案涉及治疗性选择和/或设计的方法 将减少旨在抑制分枝杆菌酶的候选药物的任何潜在毒性副作用的化合物。

公开(公告)号：US06391928B1

公开(公告)日：2002-05-21

申请号：US09570539

申请日：2000-05-12

Applicant: Ram Rajasekharan ,  Jaiyanth Daniel

Inventor： Ram Rajasekharan ,  Jaiyanth Daniel

IPC: A23L105

CPC classification number: C11B15/00 ,  A23L27/70 ,  B01J13/0065 ,  Y10S514/944

Abstract: This invention relates to a novel synergistic reversible solid/semi-solid organic composition, said composition comprising (a) at least one saturated long chain fatty acid and/or its glycerol esters and (b) one or more liquid neutral organic compounds, said ingredients (a) and (b) being present in a ratio between 0.1 to 40% by weight, and a process for producing said synergistic reversible solid/semi-solid organic composition by mixing the ingredients (a) and (b) defmed above in a ratio between 0.1 to 40% by weight, at a temperature between 2 to 50° C. and at a pressure in the range of 200 torr to 2500 torr.

Abstract translation: 本发明涉及一种新的协同可逆固体/半固体有机组合物，所述组合物包含（a）至少一种饱和长链脂肪酸和/或其甘油酯和（b）一种或多种液体中性有机化合物，所述成分 （a）和（b）以0.1-40重量％的比例存在，以及通过将上述定义的成分（a）和（b）混合在一起制备所述协同可逆固体/半固体有机组合物的方法 的比例为0.1〜40重量％，温度为2〜50℃，压力为200乇〜2500乇。

公开(公告)号：US08241644B2

公开(公告)日：2012-08-14

申请号：US12870945

申请日：2010-08-30

Applicant: Pappachan E. Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

Inventor： Pappachan E. Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

IPC: A61K39/04 ,  A61K39/02 ,  C12Q1/02

CPC classification number: C12N1/36 ,  C12N1/20

Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.

Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。

公开(公告)号：US20110033914A1

公开(公告)日：2011-02-10

申请号：US12870945

申请日：2010-08-30

Applicant: Pappachan Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

Inventor： Pappachan Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

IPC: C12N1/20

CPC classification number: C12N1/36 ,  C12N1/20

Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.

Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。

公开(公告)号：US07601357B2

公开(公告)日：2009-10-13

申请号：US11561460

申请日：2006-11-20

Applicant: Pappachan E. Kolattukudy ,  Chirajyoti Deb ,  Jaiyanth Daniel

Inventor： Pappachan E. Kolattukudy ,  Chirajyoti Deb ,  Jaiyanth Daniel

IPC: A61K39/04 ,  A61K39/00 ,  C07H21/04

CPC classification number: C12Q1/34 ,  A61K38/00 ,  C07K14/35 ,  C12N9/20 ,  G01N2500/00

Abstract: Disclosed herein are novel methods for screening for compounds useful in treating or preventing tuberculosis. In exemplary embodiments, screening methods are based on the implementation or manipulation of triacylglycerol hydrolase like polypeptides or polynucleotides encoding the same. The methods are useful in identifying agents active against TB infection.

Abstract translation: 本文公开了用于筛选用于治疗或预防结核病的化合物的新方法。 在示例性实施方案中，筛选方法基于三酰基甘油水解酶如多肽或编码其的多核苷酸的实施或操作。 该方法可用于鉴定对结核感染有活性的药剂。

公开(公告)号：US20130040829A1

公开(公告)日：2013-02-14

申请号：US13572888

申请日：2012-08-13

Applicant: Pappachan E. Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajoti Deb

Inventor： Pappachan E. Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajoti Deb

IPC: C12N5/0786 ,  C12Q1/18 ,  G01N21/64 ,  C40B30/00 ,  G01N30/90 ,  C12N1/20 ,  C12Q1/68

CPC classification number: C12N1/36 ,  C12N1/20

Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.

Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，较高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导潜伏期。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。

公开(公告)号：US08012492B2

公开(公告)日：2011-09-06

申请号：US12107146

申请日：2008-04-22

Applicant: Pappachan Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

Inventor： Pappachan Kolattukudy ,  Tatiana Sirakova ,  Jaiyanth Daniel ,  Chirajyoti Deb

IPC: A61K39/04 ,  A61K39/02 ,  C12Q1/02

CPC classification number: C12N1/36 ,  C12N1/20

Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.

Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。

公开(公告)号：US20100021453A1

公开(公告)日：2010-01-28

申请号：US12546368

申请日：2009-08-24

Applicant: Pappachan E. Kolattukudy ,  Tatiana Sirakova ,  Vinod S. Dubey ,  Jaiyanth Daniel

Inventor： Pappachan E. Kolattukudy ,  Tatiana Sirakova ,  Vinod S. Dubey ,  Jaiyanth Daniel

IPC: A61K39/395 ,  C07H21/04 ,  C07K14/35 ,  A61K48/00 ,  A61P31/04 ,  A61K38/00 ,  C12N15/63 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10

CPC classification number: G01N33/5695 ,  A61K38/1709 ,  G01N2500/04 ,  G01N2500/10

Abstract: Disclosed herein are novel methods for screening for compounds useful in treating or preventing tuberculosis. In exemplary embodiments, screening methods are based on the implementation or manipulation of triacylglycerol synthase like polypeptides or polynucleotides encoding the same. The methods are useful in identifying agents active against TB infection.

Abstract translation: 本文公开了用于筛选用于治疗或预防结核病的化合物的新方法。 在示例性实施方案中，筛选方法基于三酰基甘油合酶如多肽或编码其的多核苷酸的实施或操作。 该方法可用于鉴定对结核感染有活性的药剂。