Provided is a dual-hybridization polynucleotide including a first complementary region that is complementary to the 3′-terminus of a target nucleic and a second complementary region that is complementary to the 5′-terminus of the target nucleic acid, a composition and kit including the polynucleotide, and a method of producing a nucleotide sequence complementary to the target nucleic acid. The first complementary region to be bound at the 3′-terminus of the target nucleic acid can be shortened and the target nucleic acid may be amplified with excellent specificity and/or sensitivity.