The invention discloses a lotus root cutting machine for shallow-water lotus root raw juice stability research. The invention belongs to the technical field of experimental equipment. The machine includes an outer barrel, wherein the outer barrel comprises a large barrel and a small barrel which are connected with each other; the large barrel is positioned at the front end of the small barrel; anopening is formed in the top of the small barrel; the outer wall of the small barrel is connected with two convex shells; the bottom of the outer cylinder is connected with supporting legs; the rear end of the small barrel is connected with a rotary pushing mechanism, two symmetrically-arranged peeling mechanisms are installed in the two convex shells respectively, an outlet used for lotus root peel falling is formed in the bottom of the small barrel, a slicing mechanism is installed in the large barrel, and the front end of the large barrel is connected with a first cutter. The invention further provides an operation method of the machine. Through the machine and the method, lotus roots can be conveniently sliced into strips, the workload of experimenters is reduced, and follow-up experiments are facilitated.