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发明公开
CN103290123A 一种黄牛IGF2基因单核苷酸多态性的检测方法及试剂盒 失效 - 权利终止

一种黄牛IGF2基因单核苷酸多态性的检测方法及试剂盒
摘要：
本发明公开了一种黄牛IGF2基因单核苷酸多态性的检测方法及试剂盒，以包含IGF2基因的待测黄牛全基因组DNA为模板，PCR扩增黄牛IGF2基因；通过DNA测序和限制性片段长度多态性聚合酶链反应技术（PCR-RFLP）进行基因多态性的检测与快速分型，该方法是一种在DNA水平上筛查和检测与黄牛生长性状密切相关的分子遗传标记。本发明提供的检测方法可用于黄牛生长性状的分子标记辅助选择，进而快速建立遗传资源优良的黄牛种群。本发明检测方法简单、成本低，检测结果直接、可靠，适用于对黄牛IGF2基因突变位点的大规模的筛查和诊断。
摘要（英）：

The invention discloses a detecting method and a kit of cattle IGF2 (Insulin-like Growth Factor 2) gene mononucleotide polymorphism. The detecting method comprises the following steps of: with IGF2-gene-containing cattle whole genome DNA (deoxyribonucleic acid) to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification on the cattle IGF2 gene; and detecting and quickly parting the gene polymorphism through DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. The detecting method is used for screening and detecting a molecular genetic marker closely related to cattle growth traits on DNA level. The detecting method disclosed by the invention can be used for molecular marker-assisted selection of cattle growth traits so as to quickly create cattle population with excellent genetic resources. The detecting method disclosed by the invention is simple, low in cost, direct and reliable in detecting result and fit for screening and diagnosing the cattle IGF2 gene mutation sites in large scale.

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