The invention belongs to the field of communicable disease prevention and cure, and particularly relates to a candidated autoantigen of vaccine for blocking malignant malaria transmission. According to the invention, a gene recombination method is used for constructing a plasmodium falciparum pfs25 protein dipolymer derivative, and the dipolymer derivative comprises two pfs25 protein units which are connected through a connection arm. The obtained dipolymer derivative strengthens immunogenicity of pfs25 proteins, and can arouse high antibody response in an animal body. Dipolymers can be produced by recombination yeast cells in a high-expression mode and secreted to a culture medium. An expression product with even compositions is obtained after purification, and the expression product can be applicable to mass production. The plasmodium falciparum pfs25 protein dipolymer derivative is expected to be candidated antigens of blocked vaccines of the malignant malaria transmission.