The invention discloses an anti-Pf 332-DBL sectional monoclonal antibody capable of inhibiting invasion of plasmodium falciparum. The anti-Pf 332-DBL sectional monoclonal antibody can be specifically bound with DBL sectional functional polypeptide of a Pf 332 membrane protein of the plasmodium falciparum, wherein the DBL sectional functional polypeptide of the Pf 332 membrane protein of the plasmodium falciparum has the amino acid sequence as shown in SEQ ID No.2. The anti-Pf 332-DBL sectional monoclonal antibody can be obtained through a method that splenocyte is fused with an Sp 2/0 cell byusing a DBL recombinant protein immunized mouse with an His label, and the DBL recombinant protein with a GST label is adopted for initially selecting, and the functional polypeptide is adopted for positive selecting. The anti-Pf 332-DBL sectional monoclonal antibody has a powerful function of inhibiting the invasion of polypide, and the function is stronger than that of an MP antibody which is purified from Mali human serum that is infected by the plasmodium falciparum; a monoclonal antibody incapable of being specifically bound with the functional polypeptide has relatively weak or no function of inhibiting the invasion of the polypide; and the DBL sectional functional polypeptide can be applied to the research and development of therapeutic polypeptide drugs for malaria and the development of preventive polypeptide vaccine.