公开(公告)号：US12234467B2

公开(公告)日：2025-02-25

申请号：US17559924

申请日：2021-12-22

Applicant: KEYGENE N.V.

Inventor： Peter Johannes Van Dijk ,  Diana Rigola ,  Marinus Willem Prins ,  Adrianus Johannes Van Tunen

IPC: C12N15/82 ,  C12N15/113 ,  C12N15/62 ,  C12Q1/68 ,  C12Q1/6895

Abstract: The invention provides nucleotide sequences and amino acid sequences of the Dip gene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.

公开(公告)号：US12227749B2

公开(公告)日：2025-02-18

申请号：US18352347

申请日：2023-07-14

Applicant: The Broad Institute, Inc. ,  Massachusetts Institute of Technology

Inventor： Paul Blainey ,  Jacob Borrajo ,  Mohamad Najia ,  Atray Dixit

IPC: C12N15/62 ,  C07H21/04 ,  C12Q1/6897

Abstract: The present invention provides for methods to obtain multiple information-rich samples at different time points from the same cell while minimally disrupting the cell. The subject matter disclosed herein is generally related to nucleic acid constructs for continuous monitoring of live cells. Specifically, the subject matter disclosed herein is directed to nucleic acid constructs that encode a fusion protein and a construct RNA sequence that induce live cells to self-report cellular contents while maintaining cell viability. The present invention may be used to monitor gene expression in single cells while maintaining cell viability.

公开(公告)号：US20250051823A1

公开(公告)日：2025-02-13

申请号：US18723092

申请日：2022-12-13

Applicant: ABSCI CORPORATION

Inventor： Miles Gander ,  Amy E. Doerner ,  Carla Risso ,  Gregory Hannum ,  Ariel Schwartz

IPC: C12P21/02 ,  C12N9/08 ,  C12N15/62 ,  C12N15/70

Abstract: The disclosure provides products and methods for the heterologous expression of proteins in host cells. The provided expression constructs, bacterial cells and methods produce a PnlP-1 accessory protein that increases the yield of a heterologous protein of interest.

公开(公告)号：US20250043010A1

公开(公告)日：2025-02-06

申请号：US18808411

申请日：2024-08-19

Applicant: UMC UTRECHT HOLDING B.V ,  Gadeta B.V. ,  Albert-Ludwigs-Universität Freiburg

Inventor： Jürgen Herbert Ernst Kuball ,  Anke Janssen ,  Dennis Beringer ,  Paul Fisch ,  Jose Alberto Villacorta Hidalgo

IPC: C07K16/28 ,  A61K38/00 ,  A61K39/00 ,  A61P35/00 ,  C07K14/725 ,  C07K16/30 ,  C12N5/078 ,  C12N5/0783 ,  C12N15/62 ,  C12N15/90 ,  C12Q1/6809 ,  G01N33/569

Abstract: The present invention relates to a method for identifying δT-cell (or γT-cell) receptors chains or parts thereof that mediate an anti-tumor or anti-infection response by identifying amino acid sequences comprising δT-cells (or γT-cell) receptors chains or parts thereof that are shared between different donors.

公开(公告)号：US12202880B2

公开(公告)日：2025-01-21

申请号：US17741438

申请日：2022-05-10

Applicant: THE REGENTS OF THE UNIVERSITY OF COLORADO

Inventor： Brian Freed ,  Christina Roark ,  Elizabeth Sunderhaus

IPC: C07K14/74 ,  A61K35/17 ,  A61K35/28 ,  A61K39/00 ,  A61P37/02 ,  A61P37/06 ,  C12N5/0775 ,  C12N15/62 ,  C12N15/86 ,  C12Q1/6881 ,  A61K38/00

Abstract: Methods of preventing or treating autoimmune disease are disclosed. In some cases, subjects with having or at risk of developing autoimmune disease are identified as possessing one or more autoimmunity-susceptibility HLA alleles at one or more HLA loci. In many cases, the HLA loci are selected from Class I and Class II loci, for example Class I A, B, and C, and Class II DQ, DR, and DP. In many cases, subjects suffering from or at risk of developing an autoimmune disease may be administered a plurality engineered autologous HSCs modified to carry and express a variant susceptibility allele having at least one mutation in the antigen binding cleft that alters antigen binding and/or specificity of that variant HLA molecule. In many embodiments, the engineered HSCs are CD34+ immune cells that express one or more modified HLA proteins.

公开(公告)号：US20250019461A1

公开(公告)日：2025-01-16

申请号：US18886464

申请日：2024-09-16

Applicant: Hummingbird Bioscience Pte. Ltd.

Inventor： Jerome Douglas Boyd-Kirkup ,  Piers Ingram ,  Dipti Thakkar ,  Zhihao Wu ,  Konrad Paszkiewicz ,  Vicente Sancenon ,  Siyu Guan

IPC: C07K16/32 ,  A61K39/00 ,  A61P35/00 ,  C07K14/725 ,  C07K16/28 ,  C12N15/62 ,  C12N15/85

Abstract: HER3 antigen-binding molecules are disclosed. Also disclosed are nucleic acids and expression vectors encoding, compositions comprising, and methods using, the HER3 antigen-binding molecules.

公开(公告)号：US20250009798A1

公开(公告)日：2025-01-09

申请号：US18708166

申请日：2022-11-07

Applicant: Baylor College of Medicine

Inventor： Nabil M. Ahmed ,  Jessica Morris ,  Ahmed Gad ,  Sujith Joseph

IPC: A61K35/17 ,  A61P35/00 ,  C07K14/705 ,  C07K14/725 ,  C12N5/0783 ,  C12N15/62 ,  C12N15/86 ,  C12N15/87

Abstract: Disclosed herein, in some aspects, are immune cells comprising one or more engineered antigen receptors and one or more non-canonical CD6 isoforms and/or canonical CD6. Also disclosed are methods for cancer treatment comprising administering such immune cells to a subject in need thereof. Further disclosed are nucleic acids encoding a chimeric antigen receptor and a non-canonical CD6 isoform, and cells harboring same.

公开(公告)号：US12188063B2

公开(公告)日：2025-01-07

申请号：US17428477

申请日：2020-01-23

Applicant: INSTITUTE OF SCIENCE TOKYO

Inventor： Hiroshi Ueda ,  Jiulong Su ,  Tetsuya Kitaguchi ,  Yuki Ohmuro

IPC: C12N9/24 ,  C07K19/00 ,  C12N15/62 ,  C12Q1/34

Abstract: Provided as a mutant of β glucuronidase suitable for homogeneous immunoassays is a β glucuronidase mutant, wherein, in the amino acid sequence of Escherichia coli β glucuronidase, methionine at position 516 is substituted with lysine, and tyrosine at position 517 is unsubstituted or substituted with a non-tyrosine aromatic amino acid.

公开(公告)号：US12187778B2

公开(公告)日：2025-01-07

申请号：US17823223

申请日：2022-08-30

Applicant: INSTIL BIO (UK) LIMITED

Inventor： John Bridgeman ,  Robert Hawkins ,  Ruben Rodriguez ,  Gray Kueberuwa

IPC: C07K14/725 ,  A61K39/00 ,  C07K14/705 ,  C12N5/0783 ,  C12N15/62 ,  C12N15/86

Abstract: The present invention relates to a chimeric costimulatory antigen receptor (CoStAR) useful in adoptive cell therapy (ACT), and cells comprising the CoStAR. The CoStAR can act as a modulator of cellular activity enhancing responses to defined antigens. The present invention also provides CoStAR proteins, nucleic acids encoding the CoStAR and therapeutic uses thereof.

公开(公告)号：US12187769B2

公开(公告)日：2025-01-07

申请号：US18323090

申请日：2023-05-24

Applicant: AUTOLUS LIMITED

Inventor： Martin Pulé ,  Shaun Cordoba ,  Simon Thomas ,  Shimobi Onuoha ,  Matteo Righi

IPC: C12N15/62 ,  A61K35/17 ,  A61K39/00 ,  C07K14/47 ,  C07K14/705 ,  C12N5/0783

Abstract: The present invention relates to a cell comprising a chimeric antigen receptor (CAR) and a constitutively active or inducible Signal Transducer and Activator of Transcription (STAT) molecule.