公开(公告)号：US20030032201A1

公开(公告)日：2003-02-13

申请号：US09925430

申请日：2001-08-10

发明人： Robert W. Flesher

IPC分类号： G01N033/559 ,  G01N027/26 ,  G01N033/557

CPC分类号： G01N27/44704 ,  G01N27/44717

摘要： This application relates to a method and apparatus for the electrophoresis, blotting and detection of nucleic acids, proteins and other biological molecules using a porous polymer gel carrier and assembly. The combination of a porous polymer gel carrier and attached laminate layer facilitates the electrophoresis and subsequent blotting analysis of nucleic acids, proteins and other biological molecules. The porous polymer gel carrier also facilitates the use, handling and processing of fragile separation materials such as polyacrylimide and agarose gels. The method and apparatus also permits the convenient packaging and distribution of standardized electrophoresis and blotting products.

摘要翻译： 本申请涉及使用多孔聚合物凝胶载体和组合件对核酸，蛋白质和其他生物分子的电泳，印迹和检测的方法和装置。 多孔聚合物凝胶载体和附着的层压层的组合促进了核酸，蛋白质和其他生物分子的电泳和随后的印迹分析。 多孔聚合物凝胶载体还有助于易碎分离材料如聚丙烯酰亚胺和琼脂糖凝胶的使用，处理和加工。 该方法和装置还允许方便地包装和分配标准化的电泳和印迹产品。

公开(公告)号：US20020045160A1

公开(公告)日：2002-04-18

申请号：US09888681

申请日：2001-06-25

申请人： GenVec, Inc.

发明人： Miguel E. Carrion ,  Marilyn C. Menger

IPC分类号： C12Q001/70 ,  C12N013/00 ,  G01N033/558 ,  G01N033/559 ,  G01N033/561

CPC分类号： G01N21/6486 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10351

摘要： The present invention provides methods of detecting and/or characterizing the viral vector particle content of a medium. A medium is provided and contacted with an excitation energy such that, if a viral vector particle is in the medium, an electron associated with the intrinsically fluorogenic portion of the viral vector particle will be raised to an excited energy state. The excited electron is permitted to emit radiation having an emission wavelength which is detected. The viral vector particle content of the medium then can be evaluated by comparing the detected emission wavelength with a standard signal. For example, the number of viral vector particles in a medium can be quantified by comparing the detected wavelength and its corresponding intensity to a standard signal. Similar methods for evaluating the adenoviral vector particle content of a medium and the intrinsically fluorogenic adenoviral structural protein content of a medium are provided.

摘要翻译： 本发明提供了检测和/或表征介质的病毒载体颗粒含量的方法。 提供介质并与激发能接触，使得如果病毒载体颗粒在介质中，与病毒载体颗粒的固有荧光部分相关的电子将被升高到激发能态。 被激发的电子被允许发射具有检测到的发射波长的辐射。 然后可以通过将检测到的发射波长与标准信号进行比较来评估介质的病毒载体颗粒含量。 例如，可以通过将检测到的波长及其对应的强度与标准信号进行比较来量化介质中病毒载体颗粒的数量。 提供了用于评估培养基的腺病毒载体颗粒含量和介质的固有荧光腺病毒结构蛋白含量的类似方法。

公开(公告)号：US20040168917A1

公开(公告)日：2004-09-02

申请号：US10479245

申请日：2003-12-01

发明人： Mari Tabuchi ,  Yoshinobu Baba

IPC分类号： G01N033/559

CPC分类号： G01N27/44747

摘要： A separation carrier comprising null-1,3-glucan and/or methyl cellulose; a running buffer comprising the separation carrier; a method of capillary electrophoresis or microchip electrophoresis, wherein a sample comprising macromolecular compounds is run in the presence of the running buffer; a method of capillary electrophoresis or microchip electrophoresis, comprising the step of injecting the sample by an electrical injection or application of pressure; and a method for analyzing macromolecular compounds by the method of capillary electrophoresis or microchip electrophoresis. According to the present invention, it is possible to achieve high resolution quickly. Therefore, the methods are useful in the High Through-put screening analysis of proteins or sugar chains in gene analysis, proteome analysis or glycome analysis, and applicable in medical diagnostic apparatuses and the elucidation of biological functions, mechanisms of onset of diseases, etc.

摘要翻译： 包含β-1,3-葡聚糖和/或甲基纤维素的分离载体; 包括分离载体的运行缓冲器; 毛细管电泳或微芯片电泳的方法，其中包含高分子化合物的样品在运行缓冲液存在下运行; 一种毛细管电泳或微芯片电泳的方法，包括通过电注射或施加压力注射样品的步骤; 以及通过毛细管电泳或微芯片电泳法分析高分子化合物的方法。 根据本发明，可以快速地实现高分辨率。 因此，该方法在蛋白质或糖链的高通量筛选分析中可用于基因分析，蛋白质组分析或者糖蛋白分析，适用于医学诊断仪器，阐明生物学功能，疾病发病机制等。

公开(公告)号：US20030013134A1

公开(公告)日：2003-01-16

申请号：US09256497

申请日：1999-02-23

发明人： MARK ROTH

IPC分类号： G01N033/567 ,  G01N033/53 ,  G01N033/537 ,  G01N033/543 ,  G01N033/564 ,  G01N033/559

CPC分类号： G01N33/564 ,  G01N2800/24 ,  Y10S977/959

摘要： The present invention relates to diagnostic applications. For autoimmune diseases more particularly, it is demonstrated herein that individuals with SLE, APLA, MCDS and PSS have antibodies that are specific for SR proteins. Thus, in particular aspects the present invention provides methods and compositions for diagnosing autoimmune disease using SR proteins and antibodies to detect the presence of SR protein-specific antibodies in an individual suspected of having autoimmune disease, wherein the presence of such antibodies is indicative of said individual suffering from autoimmune disease.

摘要翻译： 本发明涉及诊断应用。 对于自身免疫性疾病更具体地，本文证明具有SLE，APLA，MCDS和PSS的个体具有对SR蛋白特异的抗体。 因此，在特定方面，本发明提供了使用SR蛋白和抗体诊断自身免疫疾病的方法和组合物，以检测在怀疑患有自身免疫性疾病的个体中SR蛋白特异性抗体的存在，其中所述抗体的存在表示所述 个体患有自身免疫性疾病。

公开(公告)号：US20020168643A1

公开(公告)日：2002-11-14

申请号：US09851540

申请日：2001-05-08

发明人： Jamey M. Wierzbowski ,  Alan Dupes

IPC分类号： C12Q001/68 ,  C12M001/34 ,  G01N033/559 ,  G01N033/561

CPC分类号： G01N33/561 ,  B01L3/5025 ,  C12N15/101 ,  C12Q1/6806 ,  G01N27/44704 ,  G01N27/44717 ,  G01N27/44782 ,  G01N33/559 ,  C12Q2523/307

摘要： This invention provides devices and methods for the simultaneous separation and purification of molecular samples by electrophoresis. In a preferred embodiment, the separation and purification of molecular samples is provided in a two-dimensional array. In a further embodiment of the invention, gas removal passageways are provided to remove gas produced by electrodes that are immersed in aqueous solution.

摘要翻译： 本发明提供了通过电泳同时分离和纯化分子样品的装置和方法。 在优选的实施方案中，以二维阵列提供分子样品的分离和纯化。 在本发明的又一个实施例中，提供了气体去除通道，以去除浸在水溶液中的电极产生的气体。

公开(公告)号：US20040101973A1

公开(公告)日：2004-05-27

申请号：US10381029

申请日：2003-04-01

发明人： Gerhard Weber

IPC分类号： G01L001/20 ,  G01N027/26 ,  G01N033/558 ,  G01N033/559

CPC分类号： G01N27/44795 ,  G01N27/44747

摘要： The invention relates to a medium for analytic and preparative electrophoresis comprising a content of acids and bases of different pKS values. Said medium contains at least two acids, whose pKS values (nullpKS) of adjacent acids are no greater than 1.0 but preferably range from 0.8 to 0.5, and contains at least two bases, whose pKS values range from approximately 1.5 to 11, whereby the difference of the pKS values (nullpKS) of adjacent bases is no greater than 1.0 but preferably ranges from 0.8 to 0.5.

摘要翻译： 本发明涉及用于分析和制备电泳的培养基，其包含不同pKS值的酸和碱的含量。 所述培养基含有至少两种酸，其相邻酸的pKS值（DeltapKS）不大于1.0，优选为0.8至0.5，并且含有至少两个碱基，其pKS值范围为约1.5至11，由此差异 的相邻碱基的pKS值（DeltapKS）不大于1.0，但优选在0.8至0.5的范围内。

公开(公告)号：US20040050700A1

公开(公告)日：2004-03-18

申请号：US10415950

申请日：2003-10-20

发明人： Lilia Lopez-Canovas ,  Ana Maria Riveron Rojas ,  David Higgison Clarke ,  Axel Sanchez Alonso ,  Esther Orozco Orozco ,  Oscar Arencibia Diaz ,  Maria Concepcion Ariosa Acuna ,  Hilda Teresa Clark Donderiz ,  Regnar Gigato Perez

IPC分类号： G01L001/20 ,  C07K001/26 ,  C02F001/469 ,  C25B007/00 ,  G01N027/26 ,  G01N033/559

CPC分类号： C12Q1/6806 ,  G01N27/44773

摘要： A process for rapid typing of yeast, parasites and bacteria is provided. It comprises the following steps: a) Preparing immobilized intact DNA in 5 to 60 minutes aided by a method involving a reagent kit that only contains buffer solution, a detergent, a metal chelating agent and an agent to disrupt the hydrogen bonds. b) Separating intact DNA molecules or their restriction fragments in miniequipments for Pulsed Field Gel Electrophoresis of the CHEF (Contour Clamped Homogeneous Electric Field) and TAFE (Transversal Alternating Field Electrophoresis) systems in times comprised between 2.5 and 7 hours. c) Selecting the optimal conditions that should be set in miniCHEF with the aid of a method to simulate the electrophoresis patterns d) Providing reorientation times, migration velocities and sizes of the molecules calculated with the aid of a method to analyze the migrated distances without the use of size markers.

摘要翻译： 提供了快速分型酵母，寄生虫和细菌的方法。 它包括以下步骤：a）通过涉及仅含有缓冲溶液的试剂盒，洗涤剂，金属螯合剂和破坏氢键的试剂的方法在5至60分钟内制备固定的完整DNA。 b）在2.5-7小时之间的时间内，将CHEF（轮廓钳式均匀电场）和TAFE（横向交替场电泳）系统的脉冲场凝胶电泳分离成小分类的完整DNA分子或其限制性片段。 c）借助于模拟电泳图案的方法选择应在miniCHEF中设置的最佳条件d）提供重新定向时间，迁移速度和分子大小，借助于分析迁移距离而不使用 使用大小标记。

公开(公告)号：US20030173220A1

公开(公告)日：2003-09-18

申请号：US10380081

申请日：2003-03-10

发明人： Bengt Bjellqvist ,  Ronnie Palmgren

IPC分类号： G01N033/559

CPC分类号： G01N27/44795 ,  G01N27/44747

摘要： An electrophoresis separation material, especially for isoelectric focusing, comprising a carrier material to which pH-buffering groups are firmly attached and anode side and cathode sides. At least a first portion of the pH-buffering groups (a) having a pH-dependent charge on a nitrogen atom binding to an sp2-hybridised carbon atom,(b) including groups with pKanull9.5. One embodiment, the groups in (a) have the structure null(NH)nC(nullNH)(NH2)(I), possibly in protonated form, where n is 0 or 1, and the free valence binds to the carrier material via an organic linker. An IEF separation material obtained by polymerisation of a mixture of monomers and containing immobilised buffering groups capable of establishing an immobilised pH-gradient where(1) at least one monomer has the structure CH2nullCHRnullCONR12R13 (V) where (i) Rnull represents hydrogen or methyl; (ii) one or more of R12 and R13 are null((CH2)nnullnullO)nnullnullH where nnull is 2-3 and nnull is 1-5 while any remaining group R12 and R13, is hydrogen and when n is 3, a hydrogen on the middle carbon in (CH2)n may be replaced with hydroxy; and (2) the immobilised pH gradient extends to pH>10.

摘要翻译： 一种电泳分离材料，特别是用于等电聚焦的电泳分离材料，其包括牢固地连接有pH-缓冲基团的载体材料和阳极侧和阴极侧。 至少第一部分pH缓冲基团（a）在与sp2杂交的碳原子结合的氮原子上具有pH依赖性电荷，（b）包括pKa = 9.5的基团。 一个实施方案中，（a）中的基团具有结构 - （NH）nC（= NH）（NH 2）（I）），可能是质子化形式，其中n为0或1，并且游离价键通过 有机接头。 通过聚合单体混合物并含有能够建立固定化pH梯度的固定化缓冲基团获得的IEF分离材料，其中（1）至少一种单体具有CH 2 = CHR'CONR 12 R 13（V）的结构，其中（i）R “代表氢或甲基; （ii）R 12和R 13中的一个或多个为 - （（CH 2）n'-O）n“-H，其中n'为2-3且n”为1-5，而任何剩余的基团R12和R13为 氢，当n为3时，（CH2）n中的中碳上的氢可被羟基取代; 和（2）固定的pH梯度延伸至pH> 10。

公开(公告)号：US20030098235A1

公开(公告)日：2003-05-29

申请号：US10278745

申请日：2002-10-21

申请人： Bio-Rad Laboratories, Inc.

发明人： Mingde Zhu ,  Lee Olech

IPC分类号： G01N033/558 ,  C07K001/00 ,  G01N033/559

CPC分类号： G01N33/54366 ,  G01N33/6839 ,  G01N2021/6439 ,  Y10S435/968 ,  Y10S436/80 ,  Y10T436/13

摘要： The proteins in a biological sample that is sought to be analyzed for its protein composition by am electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

摘要翻译： 试图通过电泳或色谱法分析其蛋白质组成的生物样品中的蛋白质以非常有效的方式通过将样品与含有染料，缓冲剂和 在优选的实施方案中，还可以使用变性剂。 组合物的固体和干燥形式可防止染料劣化或分解，组合物中组分的组合允许染料以相对均匀的方式偶联蛋白质，当组合物和样品为 一起加热并在高温下保持短时间。

公开(公告)号：US20040251137A1

公开(公告)日：2004-12-16

申请号：US10457327

申请日：2003-06-10

申请人： Rigel Technology Corporation

发明人： John H. Lee ,  Jane Q. Lee

IPC分类号： C25B007/00 ,  G01N033/559 ,  B01D057/02

CPC分类号： G01N27/44756 ,  G01N27/44704 ,  G01N27/44773

摘要： New slope electrophoresis method and apparatus for performing electrophoresis as a biochemical analysis tool is disclosed. Slope electrophoresis combines the advantages of vertical electrophoresis and horizontal electrophoresis. The angle of slope electrophoresis to the bottom side is between horizontal electrophoresis (0 degree) and vertical electrophoresis (90 degree). The most prefer angle of slope of electrophoresis is between 75 degree to 88 degree. Slope electrophoresis is also used for two-dimensional electrophoresis.

摘要翻译： 公开了一种新的斜面电泳方法和用于进行电泳作为生化分析工具的装置。 斜面电泳结合了垂直电泳和水平电泳的优点。 斜面电泳到底侧的角度在水平电泳（0度）和垂直电泳（90度）之间。 电泳斜率最优选的角度在75度到88度之间。 斜率电泳也用于二维电泳。