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    DETECTING BABESIA SPECIES NUCLEIC ACID IN A SAMPLE
    5.
    发明公开
    DETECTING BABESIA SPECIES NUCLEIC ACID IN A SAMPLE 审中-公开

    公开(公告)号：US20230227922A1

    公开(公告)日：2023-07-20

    申请号：US18159218

    申请日：2023-01-25

    Applicant: Gen-Probe Incorporated

    Inventor： Vanessa BRES ,  Deanna SELF ,  Jeffrey M. LINNEN

    IPC: C12Q1/6893 ,  C12Q1/6806

    CPC classification number: C12Q1/6893 ,  C12Q1/6806 ,  C12Q2600/16 ,  C12Q2600/166

    Abstract: There is described herein a method for specifically detecting Babesia species nucleic acid in a sample, which in one aspect comprises: (1) contacting a sample, said sample suspected of containing Babesia species nucleic acid, with at least two oligomers for amplifying a target region of a Babesia species target nucleic acid, wherein the at least two amplification oligomers comprise: (a) a first amplification oligomer comprising a first target-hybridizing sequence (i) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:66 and comprises SEQ ID NO:56 or 57; or (ii) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:96 and comprises SEQ ID NO:101; or (iii) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:97 and comprises SEQ ID NO:101; (iv) comprises or consists of SEQ ID NO:8; (v) comprises or consists of SEQ ID NO:83 and (b) a second amplification oligomer comprising a second target-hybridizing sequence that is from about 15 to about 33 contiguous nucleotides in length, and (i) is contained in SEQ ID NO:68 and comprises SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, or SEQ ID NO:85; or (ii) is contained in SEQ ID NO:67 and comprises SEQ ID NO:45 or SEQ ID NO:52; or (iii) is contained in SEQ ID NO:70 and comprises SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, or SEQ ID NO:51; (2) performing an in vitro nucleic acid amplification reaction, wherein any Babesia target nucleic acid present in said sample is used as a template for generating an amplification product; and (3) detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Babesia species target nucleic acid in said sample.

    Methods for real-time multiplex isothermal detection and identification of bacterial, viral, and protozoan nucleic acids
    6.
    发明授权
    Methods for real-time multiplex isothermal detection and identification of bacterial, viral, and protozoan nucleic acids 有权

    公开(公告)号：US11473155B2

    公开(公告)日：2022-10-18

    申请号：US16550205

    申请日：2019-08-24

    Applicant: Dougbeh-Chris Nyan

    Inventor： Dougbeh-Chris Nyan

    IPC: C12Q1/68 ,  C12Q1/70 ,  C12Q1/689 ,  C12Q1/6806 ,  C12Q1/6893 ,  C12Q1/6888

    Abstract: Herein disclosed are rapid real-time isothermal multiplex methods of detecting, identifying and quantifying bacterial, viral, and protozoan nucleic acids in a sample. These include contacting the sample with two or more sets of pathogen-specific reverse transcription loop-mediated isothermal amplification primers and novel oligofluorophores specific for the target bacterial, viral, and parasitic nucleic acids of interest such as human immunodeficiency virus, Ebola virus, Marburg virus, Yellow fever virus, hepatitis-B virus, Lassa fever virus, Plasmodium, hepatitis-C virus, hepatitis-E virus, dengue virus, Chikungunya virus, Japanese Encephalitis virus, Middle Eastern Respiratory Syndrome Corona virus, Mycobacterium, West Nile virus, Cytomegalovirus, Parvovirus, Leishmania, Trypanosoma, and Zika virus nucleic acids, under conditions sufficient to produce detectable real-time amplification signals in about 10 to 40 minutes. The amplification signals are produced by pathogen-specific fluorogenic labels included in one or more of the primers. Also, novel reaction and sample lysis buffers, primers, and kits for rapid multiplex detection, quantification, and identification of bacterial, viral, and protozoan nucleic acids by real-time isothermal amplification are herein disclosed.

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