公开(公告)号：US06441197B1

公开(公告)日：2002-08-27

申请号：US09487830

申请日：2000-01-20

Applicant: Tetsuo Nagano ,  Hirotatsu Kojima

Inventor： Tetsuo Nagano ,  Hirotatsu Kojima

IPC: C07D31182

CPC classification number: C07D493/10 ,  Y10T436/177692

Abstract: A compound represented by the following formula (I): wherein R1 and R2 represent amino group that substitute at adjacent positions on the phenyl ring, provided that either of R1 and R2 represents a mono(C1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group; and R3 and R4 independently represent hydrogen atom or an acyl group, and an agent for measurement of nitrogen monoxide which comprises said compound.

Abstract translation: 由下式（I）表示的化合物：其中R 1和R 2表示在苯环的相邻位置取代的氨基，条件是R 1和R 2中的任一个表示单（C 1-6烷基）取代的氨基，并且 其它表示未取代的氨基; 并且R 3和R 4独立地表示氢原子或酰基，以及包含所述化合物的一氧化氮测量剂。

公开(公告)号：US06448407B1

公开(公告)日：2002-09-10

申请号：US09704966

申请日：2000-11-01

Applicant: Linda G. Lee ,  Meng C. Taing ,  Barnett B. Rosenblum

Inventor： Linda G. Lee ,  Meng C. Taing ,  Barnett B. Rosenblum

IPC: C07D31182

CPC classification number: C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C09B11/08 ,  C09B11/24 ,  G01N33/533

Abstract: Substantially pure atropisomers of xanthene compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.

Abstract translation: 公开了呫吨化合物的基本纯的阻转异构体。 各种分子生物学应用利用无异构呫吨荧光染料作为底物的标记，例如核苷酸，核苷，多核苷酸，多肽和碳水化合物。 方法包括DNA测序，DNA片段分析，PCR，SNP分析，寡核苷酸连接，扩增，minisequencing和引物延伸。

公开(公告)号：US06403812B1

公开(公告)日：2002-06-11

申请号：US09580754

申请日：2000-05-30

Applicant: Steven M. Menchen ,  Linda G. Lee ,  Charles R. Connell ,  N. Davis Hershey ,  Vergine Chakerian ,  Sam L. Woo ,  Steven Fung

Inventor： Steven M. Menchen ,  Linda G. Lee ,  Charles R. Connell ,  N. Davis Hershey ,  Vergine Chakerian ,  Sam L. Woo ,  Steven Fung

IPC: C07D31182

CPC classification number: C07H21/00 ,  A61K48/00 ,  C07D311/82 ,  C09B11/08 ,  C12Q1/6816 ,  C12Q1/6869 ,  Y10S435/968 ,  Y10S436/80 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/147777 ,  C12Q2563/107

Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dicloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.

Abstract translation: 提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。 染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和6-）羧基荧光素。 提供了在DNA分析中使用染料的方法和试剂盒。

公开(公告)号：US06245807B1

公开(公告)日：2001-06-12

申请号：US09028400

申请日：1998-02-24

Applicant: Arthur Pardee ,  Chiang J. Li

Inventor： Arthur Pardee ,  Chiang J. Li

IPC: C07D31182

CPC classification number: A61K31/352

Abstract: We have now discovered that unexpectedly compounds of the following formulae I or 11 can be used to selectively stimulate the death of mammalian prostate cells, including both epithelial cell and prostate cancer cells, and thus are useful in treating prostate diseases: wherein R and R1 are each independently selected from the group consisting of hydrogen, hydroxy, thio (SH), halogen, substituted and unsubstituted alkyl, substituted and unsubstituted alkenyl, substituted and unsubstituted aryl, and substituted and unsubstituted alkoxy, and salts thereof, wherein the dotted double bond between the ring carbons to which R and R1 are bonded represent an optional ring double bond. Preferred compounds of formula I include those in which at least one of the substituents R and R1 is hydrogen and/or at least one of said substituents is allyl. Specifically preferred compounds include &bgr;-lapachone (i.e., R and R1 both being hydrogen), allyl-&bgr;-lapachone, particularly 3-allyl-&bgr;-lapachone (i.e. R being allyl and R1 being hydrogen) and 3-bromo-&bgr;-lapachone (i.e. R being bromo and R1 being hydrogen).

Abstract translation: 我们现在已经发现意想不到的是，下列化学式I或11的化合物可用于选择性刺激哺乳动物前列腺细胞，包括上皮细胞和前列腺癌细胞的死亡，因此可用于治疗前列腺疾病：其中R和R1是 各自独立地选自氢，羟基，硫代（SH），卤素，取代和未取代的烷基，取代和未取代的烯基，取代和未取代的芳基，以及取代和未取代的烷氧基及其盐，其中， R和R1键合的环碳代表任选的环双键。 优选的式I化合物包括其中至少一个取代基R和R 1为氢和/或至少一个所述取代基的化合物为烯丙基。 特别优选的化合物包括β-拉帕酮（即，R和R 2都是氢），烯丙基-β-拉帕酮，特别是3-烯丙基-β-拉帕酮（即，R是烯丙基，R 1是氢）和3-溴-β-拉帕酮 （即R为溴，R 1为氢）。

公开(公告)号：US06204396B1

公开(公告)日：2001-03-20

申请号：US09472596

申请日：1999-12-29

Applicant: Hans W. Rasmussen ,  Lawrence H. Allen

Inventor： Hans W. Rasmussen ,  Lawrence H. Allen

IPC: C07D31182

CPC classification number: C05F11/02 ,  C05D3/00

Abstract: A method produces calcium fulvate from naturally-occurring humus material, such as leonardite, or humic shales. The humus material containing solid fulvic acid and solid humic acid is mixed with water and sodium hydroxide for a first selected period of time to form a solution having a pH of approximately 10.0 thereby solubilizing the fulvic acid and the humic acid. The pH of the solution is reduced to approximately 4.5 and is maintained at the approximately pH 4.5 for second selected period of time thereby precipitating the humic acid as a solid while the fulvic acid remains in solution. The fulvic acid solution is separated from the solid humic acid. The pH of the fulvic acid solution is increased to approximately 13 with calcium hydroxide thereby precipitating the solubilized fulvic acid as calcium fulvate.

Abstract translation: 一种方法是从天然存在的腐殖质材料（如白云岩）或腐殖质页岩中产生富含钙的钙。 将含有固体富里酸和固体腐殖酸的腐殖质与水和氢氧化钠混合第一选定的时间以形成pH为约10.0的溶液，从而溶解富里酸和腐殖酸。 溶液的pH降低至约4.5，并在第二选定时间段内保持在约pH 4.5，从而使腐殖酸作为固体沉淀，而富里酸保留在溶液中。 将黄腐酸溶液与固体腐殖酸分离。 用氢氧化钙将富里酸溶液的pH增加至约13，从而使溶解的富里酸作为富钙酸盐沉淀。

公开(公告)号：US06800765B2

公开(公告)日：2004-10-05

申请号：US10108656

申请日：2002-03-27

Applicant: Zhenjun Diwu ,  Jesse J. Twu ,  Guoliang Yi ,  Luke D. Lavis ,  Yen-Wen Chen ,  Kelly J. Cassutt

Inventor： Zhenjun Diwu ,  Jesse J. Twu ,  Guoliang Yi ,  Luke D. Lavis ,  Yen-Wen Chen ,  Kelly J. Cassutt

IPC: C07D31182

CPC classification number: C07D493/10 ,  C07D311/82 ,  G01N1/30 ,  G01N33/84 ,  G01N2001/302 ,  G01N2800/04

Abstract: Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2′,7′-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.

Abstract translation: 用于测量pH的系统，包括组合物和方法，特别是在细胞，细胞器和其它样品中。 组合物包括pH敏感的荧光和荧光2'，7'-二烷基荧光素衍生物和相关的非荧光前体化合物。 组合物可以允许在激发光谱和发射光谱中进行比例测量。 所述方法包括向样品池中加入前体化合物，孵育样品池以释放游离指示物，照射样品池，以及检测游离指示剂的荧光响应。

公开(公告)号：US06605731B2

公开(公告)日：2003-08-12

申请号：US10264389

申请日：2002-10-04

Applicant: Rajnish Batlaw ,  Patrick D. Moore

Inventor： Rajnish Batlaw ,  Patrick D. Moore

IPC: C07D31182

CPC classification number: C09D11/102 ,  Y10T428/24802 ,  Y10T428/31779 ,  Y10T428/3179

Abstract: Novel addition products of isocyanates with oxyalkylene-substituted aminophenol compounds as intermediates for the production of urethane-substituted xanthene colorants, particularly triphenylmethane derivatives such as rhodarnines, are provided. The xanthene colorants exhibit improved wax and/or oil solubility and high purity. The urethane-substituted xanthene, colorant features very good wax and/or oil solubility, and is believed to be relatively nontoxic. A method for producing this novel colorant is also provided.

Abstract translation: 提供了具有氧化烯取代的氨基苯酚化合物的异氰酸酯作为制备氨基甲酸酯取代的呫吨着色剂，特别是三苯基甲烷衍生物如丹参的中间体的新型加成产物。 呫吨着色剂表现出改善的蜡和/或油溶性和高纯度。 氨基甲酸酯取代的呫吨，着色剂具有非常好的蜡和/或油溶性，并且被认为是相对无毒的。 还提供了生产这种新型着色剂的方法。

公开(公告)号：US06387512B1

公开(公告)日：2002-05-14

申请号：US09787362

申请日：2001-03-16

Applicant: David Allan Clarke ,  Bernard Mark Heron ,  Christopher David Gabbutt ,  John David Hepworth ,  Steven Michael Partington ,  Stephen Nigel Corns

Inventor： David Allan Clarke ,  Bernard Mark Heron ,  Christopher David Gabbutt ,  John David Hepworth ,  Steven Michael Partington ,  Stephen Nigel Corns

IPC: C07D31182

CPC classification number: C07D491/04 ,  C07D311/78 ,  C07D311/92 ,  C07D493/04 ,  C07D495/04 ,  Y10S428/913 ,  Y10T428/31855

Abstract: A photochromic grey coloring 2H-naphtho[1,2-b]pyran of formula (1) wherein R1 is selected from mono-, di- or poly-substituted aryl groups, mono-, di- or poly-substituted naphthyl groups and mono-, di- or poly-substituted heteroaryl groups, wherein at least one substituent is a nitrogen containing group, including amino, C1-C20 and C6-C20 alkylamino, C1-C20 and C6-C20 dialkylamino, C2-C20 dialkenylamino, C2-C20 or C4-C20 di(polyalkenyl)amino, arylamino, diarylamino, C1-C20 alkylarylamino, tetra (C1-C10 linear or branched alkyl) guanidino and cyclic-amino groups and at least one of R7 and R9, which may be the same or different, is selected from C1-C20 N alkylamino C1-C20 N-alkylamido, C1-C20 N,N-dialkylamido, amido, nitro, amino, C1-C20 alkylamino, C1-C20 dialkylamino, C2-C20 dialkenylamino, C4-C20 di(polyalkenyl)amino, arylamino, diarylamino, C1-C20 alkylarylamino, or cyclicamino groups. The compounds may be combined with a polymeric host material such as plastic or glass to make a sunglass lens, an ophthalmic lens or a window. The compounds may also be included in an ink or a fuel.

Abstract translation: 式（1）的光致变色灰色着色2H-萘并[1,2-b]吡喃，其中R1选自单取代，二取代或多取代的芳基，单取代，二取代或多取代的萘​​基和单 其中至少一个取代基是含氮基团，包括氨基，C 1 -C 20和C 6 -C 20烷基氨基，C 1 -C 20和C 6 -C 20二烷基氨基，C 2 -C 20二烯基氨基，C 2 -C 20烷基氨基， C20或C4-C20二（聚链烯基）氨基，芳基氨基，二芳基氨基，C1-C20烷基芳基氨基，四（C1-C10直链或支链烷基）胍基和环氨基以及R7和R9中至少一个可以相同 选自C 1 -C 20 N烷基氨基C 1 -C 20 N-烷基酰氨基，C 1 -C 20 N，N-二烷基酰氨基，酰氨基，硝基，氨基，C 1 -C 20烷基氨基，C 1 -C 20二烷基氨基，C 2 -C 20二烯基氨基， C20二（多烯基）氨基，芳基氨基，二芳基氨基，C 1 -C 20烷基芳基氨基或环氨基。 化合物可以与诸如塑料或玻璃的聚合物主体材料组合以制备太阳镜，眼镜或窗。 化合物也可以包括在油墨或燃料中。