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公开(公告)号：US20180251796A1
公开(公告)日：2018-09-06
申请号：US15755976
申请日：2015-08-28
CPC分类号： C12P5/007 , C12N9/10 , C12N9/90 , C12N15/63 , C12N15/74 , C12P23/00 , C12R1/01 , C12Y103/9903 , C12Y104/99 , C12Y202/01007 , C12Y205/01029 , C12Y205/01032 , C12Y503/03002 , C12Y505/01018
摘要： The present invention concerns a method of producing and enantiomerically pure alpha-ionone. Further, the invention concerns a nucleic acid that comprises a sequence that encodes a lycopene-epsilon-cyclase (EC), a lycopene-epsilon-cyclase (EC), plasmids, which encode components of the alpha-ionone biosynthesis and a microorganism that contains heterologous nucleotide sequences which encode the enzymes geranylgeranyl-diphosphate-synthase, isopentenyl-diphosphate-isomerase (IPI), phytoene desaturase-dehydrogenase (crtI), phytoene synthase (crtB), lycopene-epsilon-cyclase (EC) and carotenoid-cleavage-dioxygenase (CCD1). Further, the invention concerns a method of producing highly pure epsilon-carotene.
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公开(公告)号：US20240018556A1
公开(公告)日：2024-01-18
申请号：US18353673
申请日：2023-07-17
发明人： Jeffrey George LINGER , Christine Ann SINGER , Lauren Ann Riley DRUMM , Michael T. GUARNIERI , Davinia SALVACHÚA RODRÍGUEZ , Colin Matthew KNEUCKER , Violeta SANCHEZ NOGUE
CPC分类号： C12P7/52 , C12N9/88 , C12Y401/01018 , C12N9/0014 , C12Y104/99
摘要： Clostridium sp. are an obligate anaerobic bacterium that produces butyric acid as its major end-product. Previously, we have developed an integrated process that couples fermentation of biomass sugars, with in situ product recovery and distillation. This process works best at pH's lower than 7.0. The process serves to extract carboxylic acids during fermentation which enables reduced base-loading and reduced end-product toxicity. Disclosed herein are methods and non-naturally Clostridium sp. that are capable of increased production of butyric acid and increased growth at low pH conditions (e.g., pH
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