公开(公告)号：US11866720B2

公开(公告)日：2024-01-09

申请号：US17233956

申请日：2021-04-19

申请人： BASF AGRO B.V.

发明人： Raphael Aponte ,  Stefan Tresch ,  Matthias Witschel ,  Jens Lerchl ,  Dario Massa ,  Tobias Seiser ,  Thomas Mietzner ,  Jill Marie Paulik ,  Chad Brommer

IPC分类号： C12N15/82 ,  C12N9/02 ,  A01H5/00

CPC分类号： C12N15/8274 ,  A01H5/00 ,  C12N9/001 ,  C12Y103/03004

摘要： The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

公开(公告)号：US20230175005A1

公开(公告)日：2023-06-08

申请号：US18049158

申请日：2022-10-24

申请人： BASF SE

发明人： Raphael Aponte ,  Tobias Seiser ,  Stefan Tresch ,  Peter Alexamder Bowerman ,  Liliana Parra Rapado ,  Matthias Witschel ,  Laetitia Souillart ,  Manuel Johannes ,  Thomas Mietzner ,  Jill Marie Paulik

IPC分类号： C12N15/82 ,  A01N43/54

CPC分类号： C12N15/8274 ,  A01N43/54 ,  C12Y103/03004

摘要： The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

公开(公告)号：US20050260727A1

公开(公告)日：2005-11-24

申请号：US11078991

申请日：2005-03-11

申请人： Jorg Freigang ,  Michael Koch ,  Constanze Breithaupt ,  Reiner Kiefersauer ,  Albrecht Messerschmidt ,  Robert Huber

发明人： Jorg Freigang ,  Michael Koch ,  Constanze Breithaupt ,  Reiner Kiefersauer ,  Albrecht Messerschmidt ,  Robert Huber

IPC分类号： C12N9/02 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： C12N9/001 ,  C07K2299/00 ,  C12Y103/03004

摘要： The present invention relates to crystals of a protoporphyrinogen IX oxidase, to processes for preparing crystals of a protoporphyrinogen IX oxidase, to processes for determining the structure of protoporphyrinogen IX oxidases alone or complexed with a ligand, and to processes for identifying ligands of the mitochondrial protoporphyrinogen IX oxidase, which may be used as herbicides. The present invention also relates to the use of the crystal structure described here of the Nicotiana tabacum mitochondrial protoporphyrinogen IX oxidase for generating protein models of related enzymes and to the identification of inhibitors of related proteins.

摘要翻译： 本发明涉及原卟啉原IX氧化酶的晶体，用于制备原卟啉原IX氧化酶的晶体的方法，用于确定单独或与配体络合的原卟啉原IX氧化酶的结构的方法，以及用于鉴定线粒体原卟啉原配体的方法 IX氧化酶，可用作除草剂。 本发明还涉及这里描述的烟草线粒体原卟啉原IX氧化酶的晶体结构用于产生相关酶的蛋白质模型和鉴定相关蛋白质的抑制剂。

公开(公告)号：US06177245B1

公开(公告)日：2001-01-23

申请号：US09071296

申请日：1998-05-01

申请人： Eric R. Ward ,  Sandra Volrath

发明人： Eric R. Ward ,  Sandra Volrath

IPC分类号： C12Q168

CPC分类号： C12Y103/03004 ,  C12N5/04 ,  C12N9/001 ,  C12N15/8274 ,  C12Q1/6895

摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants may be bred or engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or through increased levels of expression of the native protox gene, or they may be transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Diagnostic and other uses for the novel eukaryotic protox sequence are also described. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 还提供了赋予除草剂耐受性的具有改变的排毒活性的植物。 这些植物可以通过将天然protox基因突变为抗性形式或通过增加天然protox基因的表达水平培育或设计用于抵抗前毒素抑制剂，或者可以用经修饰的真核或原核protox编码序列或野生型 类型的原核生殖毒素序列是耐除草剂。 还描述了用于新型真核毒素序列的诊断和其它用途。 还公开了编码野生型和改变的protox的植物基因，纯化的植物protox，从植物中分离protox的方法，以及使用protox编码基因的方法。

公开(公告)号：US20240271153A1

公开(公告)日：2024-08-15

申请号：US18405567

申请日：2024-01-05

申请人： BASF AGRO B.V.

发明人： Raphael Aponte ,  Stefan Tresch ,  Matthias Witschel ,  Jens Lerchl ,  Dario Massa ,  Tobias Seiser ,  Thomas Mietzner ,  Jill Marie Paulik ,  Chad Brommer

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N9/02

CPC分类号： C12N15/8274 ,  A01H5/00 ,  C12N9/001 ,  C12Y103/03004

摘要： The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

公开(公告)号：US20240200091A1

公开(公告)日：2024-06-20

申请号：US18557777

申请日：2022-04-27

申请人： BEIJING DABEINONG BIOTECHNOLOGY CO., LTD.

发明人： Xiang XIAO ,  Qingfang SONG ,  Qing TAO ,  Caihong YU ,  Xiaoming BAO

IPC分类号： C12N15/82 ,  A01N33/22 ,  A01N43/54 ,  A01N43/653 ,  A01N43/84 ,  A01P13/02 ,  C12N9/02

CPC分类号： C12N15/8274 ,  A01N33/22 ,  A01N43/54 ,  A01N43/653 ,  A01N43/84 ,  A01P13/02 ,  C12N9/001 ,  C12Y103/03004

摘要： The present invention relates to the use of a protoporphyrinogen oxidase. A method for controlling weeds comprises applying a herbicide containing an effective dose of a PPO inhibitor to a field in which at least one transgenic plant is present, wherein the transgenic plant comprises, in the genome thereof, a polynucleotide sequence encoding protoporphyrinogen oxidase, and the transgenic plant has reduced plant damage and/or has an increased plant yield compared with other plants that do not have a polynucleotide sequence encoding protoporphyrinogen oxidase. The protoporphyrinogen oxidase PPO1-PPO14 of the present invention has a high tolerance to a PPO inhibitor herbicide. In addition, the plants containing the polynucleotide sequence encoding protoporphyrinogen oxidase have a strong tolerance to the PPO-inhibitor herbicide, and show high-resistance tolerance to almost all of 4-fold field concentration of oxyfluorfen, saflufenacil and flumioxazin and 2-fold field concentration of sulfentrazone. Therefore, the protoporphyrinogen oxidase has broad application prospects in plants.

公开(公告)号：US20240026371A1

公开(公告)日：2024-01-25

申请号：US18157370

申请日：2023-01-20

申请人： BASF AGRO B.V.

发明人： Raphael Aponte ,  Stefan Tresch ,  Matthias Witschel ,  Jens Lerchl ,  Jill Marie Paulik ,  Chad Brommer

IPC分类号： C12N15/82 ,  G01N33/50 ,  C12N9/02 ,  A01N43/84

CPC分类号： C12N15/8274 ,  G01N33/5097 ,  C12N9/0059 ,  A01N43/84 ,  C12N9/0004 ,  C12Y103/03004 ,  G01N2333/90206

摘要： The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

公开(公告)号：US20150299725A1

公开(公告)日：2015-10-22

申请号：US14408439

申请日：2013-06-19

申请人： BASF SE

发明人： Jens LERCHL ,  Stefan TRESCH ,  Dario MASSA ,  Tobias SEISER ,  Matthias WITSCHEL ,  Raphael APONTE ,  Jill Marie PAULIK ,  Chad BROMMER

IPC分类号： C12N15/82 ,  A01N43/54 ,  A01N43/84 ,  C12N9/02

CPC分类号： C12N15/8274 ,  C12Y103/03004

摘要： The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

摘要翻译： 本发明涉及一种用于在植物栽培部位控制不需要的植被的方法，所述方法包括以下步骤：在所述部位提供包含至少一种核酸的植物，所述核酸包含编码野生型或突变型的核苷酸序列 通过向所述部位施用有效量的所述除草剂，对PPO抑制性除草剂具有抗性或耐受性的原卟啉原氧化酶（PPO）。 本发明还涉及包含野生型或突变型PPO酶的植物，以及获得这种植物的方法。

公开(公告)号：US20120149576A1

公开(公告)日：2012-06-14

申请号：US13377115

申请日：2010-06-09

申请人： Takanori Saijo ,  Akitsu Nagasawa

发明人： Takanori Saijo ,  Akitsu Nagasawa

IPC分类号： A01N43/54 ,  A01P13/00 ,  C12Q1/26

CPC分类号： C12N15/8274 ,  A01H5/10 ,  A01N43/54 ,  A01N63/02 ,  C12N9/001 ,  C12N9/0077 ,  C12Y103/03004

摘要： The present invention enables providing a method for cultivating a crop plant into which has been introduced either one or both of (1) DNA comprising a nucleotide sequence encoding the amino acid sequence of a cytochrome P450 that shows saflufenacil metabolizing activity and (2) DNA comprising a nucleotide sequence encoding the amino acid sequence of a protein that shows protoporphyrinogen IX oxidase activity, wherein said method comprises applying a weed control agent that contains saflufenacil as an active ingredient to an area where said crop plant is cultivated; among others.

摘要翻译： 本发明提供了一种栽培作物植物的方法，其中已经引入了（1）包含编码显示香草氟苯代谢活性的细胞色素P450的氨基酸序列的核苷酸序列的DNA中的一种或两种，（2）包含 编码显示原卟啉原IX氧化酶活性的蛋白质的氨基酸序列的核苷酸序列，其中所述方法包括将包含香草氟苯作为活性成分的杂草控制剂施用于所述作物栽培的区域; 等等

公开(公告)号：US12077768B2

公开(公告)日：2024-09-03

申请号：US17721822

申请日：2022-04-15

申请人： BASF AGRO B.V.

发明人： Raphael Aponte ,  Stefan Tresch ,  Matthias Witschel ,  Jens Lerchl ,  Dario Massa ,  Tobias Seiser ,  Thomas Mietzner ,  Jill Marie Paulik ,  Chad Brommer

IPC分类号： C12N15/82 ,  A01N37/10 ,  A01N43/54 ,  A01N43/653 ,  A01N43/84 ,  C12N9/02 ,  C12N9/10

CPC分类号： C12N15/8274 ,  A01N37/10 ,  A01N43/54 ,  A01N43/653 ,  A01N43/84 ,  C12N9/001 ,  C12N9/1059 ,  C12Y103/03004

摘要： The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.