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    METHOD AND SYSTEM FOR CONTINUOUS BIOTRANSFORMATION
    6.
    发明申请
    METHOD AND SYSTEM FOR CONTINUOUS BIOTRANSFORMATION 审中-公开
    连续生物化学方法与系统

    公开(公告)号：US20110269181A1

    公开(公告)日：2011-11-03

    申请号：US12769528

    申请日：2010-04-28

    申请人： CHEAN-YEH CHENG ,  HSIANG-RONG TSAI

    发明人： CHEAN-YEH CHENG ,  HSIANG-RONG TSAI

    IPC分类号： C12P33/00 ,  C12P1/02 ,  C12M1/00 ,  C12P1/00

    CPC分类号： C12M41/00 ,  C12M23/58 ,  C12P33/00 ,  C12P33/16

    摘要： One embodiment of the present invention discloses a method for continuous biotransformation. The method is continuously supplying viable biocatalyst cells or biocatalyst biomolecules to a bioreactor containing substrate mediums, so as to mediate the substrate mediums to be converted into the desired bioproducts.

    摘要翻译： 本发明的一个实施方案公开了一种用于连续生物转化的方法。 该方法是将可行的生物催化剂细胞或生物催化剂生物分子连续供给到含有底物培养基的生物反应器中，以便介导待转化成所需生物产物的底物培养基。

    PROCESS FOR THE ENANTIOSELECTIVE REDUCTION AND OXIDATION, RESPECTIVELY, OF STEROIDS
    9.
    发明申请
    PROCESS FOR THE ENANTIOSELECTIVE REDUCTION AND OXIDATION, RESPECTIVELY, OF STEROIDS 有权
    方法选择性减少和氧化的方法，相当于STEROIDS

    公开(公告)号：US20090280525A1

    公开(公告)日：2009-11-12

    申请号：US11910886

    申请日：2007-04-11

    申请人： Antje Gupta ,  Anke Tschentscher ,  Maria Bobkova

    发明人： Antje Gupta ,  Anke Tschentscher ,  Maria Bobkova

    IPC分类号： C12P33/02

    CPC分类号： C12P33/02 ,  C12P33/16 ,  C12P41/002

    摘要： A process for the enantioselective enzymatic reduction of compounds which comprise a steroid structure (ABCD) including one or several heteroatoms, one or several double bonds and/or one aromatic component in the ring structure and have at least one oxo group at position 3, 7, 11, 12 or 17 in the steroid ring system or in the α-position at any carbon moiety on the steroid skeleton (=oxosteroid compound), wherein the oxosteroid compound is reduced with a hydroxysteroid dehydrogenase in the presence of a cofactor NADH or NADPH, is characterized in that a) the oxosteroid compound is provided in the reaction at a concentration of ≧50 g/l, b) the oxidized cofactor NAD or NADP formed by the hydroxysteroid dehydrogenase is regenerated continuously by oxidation of a secondary alcohol of general formula RXRYCHOH, wherein RX, RY independently represent hydrogen, a branched or unbranched C1-C8-alkyl and Ctotal≧3, or by oxidation of a C4-C6-cycloalkanol, and c) an additional oxidoreductase/alcohol dehydrogenase is used for the oxidation of the secondary alcohol of general formula RXRYCHOH or of the cycloalkanol, respectively.

    摘要翻译： 包含在环结构中包含一个或几个杂原子，一个或几个双键和/或一个芳族成分并且在3,7位具有至少一个氧代基的类固醇结构（ABCD）的化合物的对映选择性酶还原的方法 ，类固醇环系统中的11,12或17，或类固醇骨架（= oxosteroid化合物）上任何碳部分的α位置，其中在辅助因子NADH或NADPH存在下，用羟基类固醇脱氢酶还原氧化甾体化合物 其特征在于a）在反应中以> = 50g / l的浓度提供氧化甾体化合物，b）由羟基类固醇脱氢酶形成的氧化辅因子NAD或NADP通过一般仲醇的氧化而连续再生 式RXRYCHOH，其中RX，RY独立地表示氢，支链或非支链C1-C8-烷基和Ctotal = 3，或通过C4-C6-环烷醇的氧化，和c）另外的氧化还原酶/ 醇脱氢酶分别用于通式RXRYCHOH的仲醇或环烷醇的氧化。