公开(公告)号：US20240344031A1

公开(公告)日：2024-10-17

申请号：US18682918

申请日：2022-08-02

申请人： UNIVERSITÄT BASEL

发明人： Sebastien PIGEOT ,  Ivan MARTIN ,  Paul BOURGINE

IPC分类号： C12N5/0775 ,  C07K14/51 ,  C12N5/077 ,  C12N9/12 ,  C12N9/64

CPC分类号： C12N5/0662 ,  C07K14/51 ,  C12N5/0655 ,  C12N9/1276 ,  C12N9/6472 ,  C12N2506/1346 ,  C12N2510/00 ,  C12N2533/90 ,  C12Y207/07049 ,  C12Y304/22036

摘要： A mesenchymal stem/stromal (MSC) cell line, in particular a human mesenchymal stem cell (hMSC), capable of chondrogenic differentiation when cultured in a chondrogenic medium, comprising a first transgene comprising a first nucleic acid sequence encoding for a preferably mammalian immortalizing enzyme under control of a first promoter sequence operable in said mesenchymal cell and a second transgene comprising a second nucleic acid sequence encoding a preferably mammalian bone morphogenic protein under control of a second promoter sequence operable in said mesenchymal cell.

公开(公告)号：US20240287463A1

公开(公告)日：2024-08-29

申请号：US17793287

申请日：2021-01-22

申请人： Massachusetts Institute of Technology ,  TRUSTEES OF BOSTON UNIVERSITY

发明人： Christopher S. Chen ,  Sangeeta N. Bhatia ,  Arnav Chhabra ,  Amanda Chen ,  Hyun Ho Song

IPC分类号： C12N5/071 ,  A61L27/38 ,  C12N5/00 ,  C12N5/077 ,  C12N9/64

CPC分类号： C12N5/0671 ,  A61L27/3886 ,  A61L27/3895 ,  C12N5/0062 ,  C12N5/0656 ,  C12N9/6472 ,  C12Y304/22062 ,  C12N2502/1323 ,  C12N2513/00

摘要： Inducible engineered tissue constructs comprising at least one cell population comprising a genetic construct are provided. Methods of making and using said constructs are also provided.

公开(公告)号：US20240182942A1

公开(公告)日：2024-06-06

申请号：US18562913

申请日：2021-05-31

申请人： ASYMCHEM LABORATORIES (TIANJIN) CO., LTD.

发明人： Hao HONG ,  Gage JAMES ,  Yi XIAO ,  Na ZHANG ,  Xuecheng JIAO ,  Junqi ZHAO ,  Lei WANG ,  Xiangyu MENG ,  Mujiao ZHANG

IPC分类号： C12P21/06 ,  C12N9/64

CPC分类号： C12P21/06 ,  C12N9/6472 ,  C12Y304/22068

摘要： The preparation method for a polypeptide includes the steps of constructing an engineering strain for fusion-expressing a polypeptide gene with a Sumo tag, and inducing the engineering strain for the soluble expression of a polypeptide, obtaining a crude protein containing a polypeptide precursor from the engineering strain by purification, cleaving the crude protein containing the polypeptide precursor by a Ulp1 protease to remove the Sumo tag, and purifying a cleavage product of the Ulp1 protease by a method of acetonitrile combined with heating precipitation or a method of precipitation with hexafluoro isopropanol to obtain the polypeptide.

公开(公告)号：US11993794B2

公开(公告)日：2024-05-28

申请号：US17203473

申请日：2021-03-16

申请人： Fred Hutchinson Cancer Center

发明人： Rupesh Amin

IPC分类号： C12N9/52 ,  C07K14/705 ,  C07K16/00 ,  C07K16/44 ,  C07K16/46 ,  C12N9/50 ,  C12N9/64 ,  C12N9/96 ,  C12N15/70 ,  C12N15/81

CPC分类号： C12N9/52 ,  C07K14/705 ,  C07K16/005 ,  C07K16/44 ,  C07K16/468 ,  C12N9/50 ,  C12N9/6472 ,  C12N9/96 ,  C07K2317/22 ,  C07K2317/32 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2319/61 ,  C07K2319/70 ,  C07K2319/71 ,  C07K2319/715 ,  C07K2319/80 ,  C12N15/70 ,  C12N15/81

摘要： Methods to create chemically-induced dimerizing (CID) protein systems and uses thereof are described. The methods utilize antibody binding domain dimerizing proteins. The created systems can be used to regulate cellular events such as gene expression, receptor signaling and cell death to effectuate a variety of clinically relevant treatment outcomes.

公开(公告)号：US11926860B2

公开(公告)日：2024-03-12

申请号：US17485606

申请日：2021-09-27

申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Hak Suk Chung ,  Jinsu An ,  Eun Gyeong Yang ,  So Yeon Kim

IPC分类号： C12Q1/02 ,  C12N9/64 ,  G01N33/58

CPC分类号： C12Q1/025 ,  C12N9/6472 ,  C12Y304/22057 ,  C12Q2527/127 ,  C12Y304/22036 ,  C12Y304/22055 ,  C12Y304/22056 ,  C12Y304/22062 ,  C12Y304/22064 ,  G01N33/582 ,  G01N2400/50 ,  G01N2500/00

摘要： Provided are a method of screening an inhibitor of caspase activity by lipopolysaccharide and a method of screening a therapeutic agent for inflammatory diseases or sepsis using the same. Accordingly, it is possible to develop a caspase-4-specific inhibitor.

公开(公告)号：US11845967B2

公开(公告)日：2023-12-19

申请号：US16867923

申请日：2020-05-06

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Henry M. Colecraft ,  Scott Kanner

IPC分类号： C12N9/64 ,  C12N15/85 ,  C12Q1/6883 ,  A61K38/00

CPC分类号： C12N9/6472 ,  C12N15/85 ,  C12Q1/6883 ,  C12Y304/22 ,  A61K38/00 ,  C12Q2600/156

摘要： The present disclosure provides, inter alia, a recombinant engineered deubiquitinase (DUB) and methods for treating or ameliorating an inherited ion channelopathy, such as long QT syndrome, Brugada syndrome, or cystic fibrosis, in a subject. Further provided are methods for screening mutations causing such inherited ion channelopathies for a trafficking-deficient mutation that is treatable by the recombinant engineered DUB disclosed herein.

公开(公告)号：US11839647B2

公开(公告)日：2023-12-12

申请号：US16734104

申请日：2020-01-03

申请人： Bellicum Pharmaceuticals, Inc.

发明人： Kevin Slawin ,  David Michael Spencer ,  Aaron Edward Foster

IPC分类号： A61K38/48 ,  A61K38/16 ,  C12N9/64 ,  C12N5/00 ,  A61K35/17 ,  A61K38/17 ,  A61K48/00 ,  A61K35/28 ,  A61K35/30 ,  A61K35/545 ,  A61K39/00

CPC分类号： A61K38/4873 ,  A61K35/17 ,  A61K35/28 ,  A61K35/30 ,  A61K35/545 ,  A61K38/16 ,  A61K38/177 ,  A61K39/0005 ,  A61K48/005 ,  C12N5/0081 ,  C12N5/0087 ,  C12N9/6472 ,  C12N2501/48 ,  C12N2510/00

摘要： The technology relates in part to methods for inducing partial apoptosis of cells that express an inducible caspase polypeptide. The technology further relates in part to methods for inducing partial apoptosis of cells that express an inducible modified caspase polypeptide, having a modified dose response curve to the multimeric ligand inducer. The technology also relates in part to methods for cell therapy using cells that express the inducible caspase polypeptide or the inducible modified caspase polypeptide, where the proportion of caspase polypeptide-expressing cells eliminated by apoptosis is related to the administered amount of the multimeric ligand inducer.

公开(公告)号：US11834698B2

公开(公告)日：2023-12-05

申请号：US16401906

申请日：2019-05-02

申请人： INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH

发明人： Sandanaraj Selvaraj Britto ,  Punita Bathla

IPC分类号： C12Q1/37 ,  G01N33/542 ,  C12N9/64 ,  G01N33/50

CPC分类号： C12Q1/37 ,  C12N9/6472 ,  G01N33/5005 ,  G01N33/542 ,  C12Y304/22001 ,  C12Y304/2206 ,  C12Y304/22056 ,  C12Y304/22061 ,  C12Y304/22062 ,  G01N2333/96466

摘要： Methods and materials for specific imaging of active enzyme in a live or intact cell are disclosed. The enzyme of interest tagged to reporter protein (donor) is exogenously expressed in a cell. The conversion of proenzyme to active enzyme (containing reporter protein) is achieved upon applying an appropriate stimulus to the target cells. The activated enzyme is labelled with an activity-based probe carrying a fluorophore (acceptor). The covalent labelling of active enzyme by the activity-based probe creates a FRET pair which provides the opportunity to exquisitely image the function of an “active enzyme”. This method is used for specific imaging of the function of active caspase-3,-7,-8,-9 and cathepsin-B and also for profiling of inhibitors of caspases and cathepsin B.

公开(公告)号：US20230302100A1

公开(公告)日：2023-09-28

申请号：US18053676

申请日：2022-11-08

申请人： Hansa Biopharma AB

发明人： Christian Kjellman ,  Sofia Jarnum ,  Emma Nordahl

IPC分类号： A61K38/48 ,  C12N9/64 ,  C12N9/24

CPC分类号： A61K38/48 ,  C12N9/24 ,  C12N9/6472 ,  C12N9/6475 ,  C12Y304/2201 ,  A61K38/00 ,  Y02A50/30

摘要： The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG.

公开(公告)号：US20230277686A1

公开(公告)日：2023-09-07

申请号：US18004372

申请日：2021-07-09

申请人： GENETHON ,  INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCH MEDICALE) ,  UNIVERSITE D'EVRY-VAL-D'ESSONNE

发明人： William LOSTAL ,  Isabelle RICHARD

IPC分类号： A61K48/00 ,  C12N15/86 ,  C12N9/64 ,  C07K14/47 ,  A61P21/00

CPC分类号： A61K48/0058 ,  C12N15/86 ,  C12N9/6472 ,  C12Y304/22054 ,  C07K14/4708 ,  C07K14/47 ,  C07K14/471 ,  A61P21/00 ,  C12N2750/14143 ,  C12N2820/007 ,  C07K2319/00

摘要： The present invention concerns a novel short promoter characterized by a high activity in the skeletal muscles and a low activity in the heart. It then constitutes a valuable candidate especially for driving the expression of transgenes encoding proteins useful for the treatment of muscular dystrophies.