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公开(公告)号：US08357523B2
公开(公告)日：2013-01-22
申请号：US12816989
申请日：2010-06-16
IPC分类号： C12N9/26
CPC分类号： C12N9/42 , C12N9/2445 , C12P7/06 , C12P19/14 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The invention provides variants of the Azospirillum irakense CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
摘要翻译： 本发明提供了与野生型酶相比具有改善的β-葡糖苷酶活性，特别是改进的热活性的Azospirillum irakense CelA和葡糖苷酶的变体。 本发明还提供了相关的多核苷酸，载体，宿主细胞，以及用于制备和使用变体的方法。
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公开(公告)号：US20130095554A1
公开(公告)日：2013-04-18
申请号：US13583238
申请日：2011-03-11
IPC分类号： C12N9/42
CPC分类号： C12N9/42 , C11D3/38645 , C11D3/38681 , C12N9/2437 , D06M16/003 , Y02E50/343
摘要： The present invention relates to a modified Family 5 cellulase comprising a substitution of an amino acid at position 363 with a non-native alanine, serine or threonine, said position determined from alignment of the modified Family 5 cellulase with amino acids 71-397 of a Trichoderma reesei Cel5A amino acid sequence as set forth in SEQ ID NO:1 and enzyme mixtures comprising same. Additionally provided is a genetic construct comprising a nucleic acid sequence encoding the modified Family 5 cellulase and a genetically modified microbe comprising the genetic construct. The invention also provides a process for producing the modified Family 5 cellulase.
摘要翻译： 本发明涉及一种修饰的家族5纤维素酶，其包含用位置363的氨基酸与非天然丙氨酸，丝氨酸或苏氨酸取代的所述位置，所述位置通过修饰的家族5纤维素酶与氨基酸71-397的比对确定 里氏木霉Cel5A氨基酸序列，如SEQ ID NO：1所示，和含有它们的酶混合物。 另外提供了包含编码修饰的家族5纤维素酶的核酸序列和包含遗传构建体的遗传修饰微生物的遗传构建体。 本发明还提供了一种制备修饰的家族5纤维素酶的方法。
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3.发明申请公开(公告)号：US20120164696A1
公开(公告)日：2012-06-28
申请号：US13416608
申请日：2012-03-09
申请人： Jie Yang , Xiyun Zhang , Attila Andor
发明人： Jie Yang , Xiyun Zhang , Attila Andor
CPC分类号： C12N9/42 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , Y02E50/16
摘要： The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.
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4.发明申请RECOMBINANT BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF SOLUBLE SUGARS FROM CELLULOSIC BIOMASS 有权用于从细胞生物量生产可溶性糖的重组β-葡萄糖苷酶变体公开(公告)号：US20110129881A1
公开(公告)日：2011-06-02
申请号：US12954447
申请日：2010-11-24
申请人： Jie Yang , Xiyun Zhang , Attila Andor
发明人： Jie Yang , Xiyun Zhang , Attila Andor
CPC分类号： C12N9/42 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , Y02E50/16
摘要： The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.
摘要翻译： 本发明涉及真菌C1菌株和葡萄糖苷酶的变体形式的重组表达。 本发明还涉及通过使用表达“葡糖苷酶”变体的转基因生物体从生物质产生可发酵糖并通过发酵糖生产生物燃料。 本发明提供了通过将纤维二糖与重组体 - 葡糖苷酶变体蛋白（例如由重组宿主细胞分泌的变体蛋白）接触到培养基中来从纤维二糖生产可发酵糖例如葡萄糖的方法。 本发明的方法可用于将生物质底物转化为可发酵的糖，最终转化为乙醇或其他生物燃料。
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公开(公告)号：US08715975B2
公开(公告)日：2014-05-06
申请号：US14018531
申请日：2013-09-05
申请人： Codexis, Inc.
CPC分类号： C12N9/42 , C12N9/2445 , C12P7/06 , C12P19/14 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The invention provides variants of the Azospirillum irakense CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
摘要翻译： 本发明提供了与野生型酶相比具有改善的β-葡糖苷酶活性，特别是改进的热活性的Azospirillum irakense CelA和葡糖苷酶的变体。 本发明还提供了相关的多核苷酸，载体，宿主细胞，以及用于制备和使用变体的方法。
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6.发明授权Recombinant β-glucosidase variants for production of soluble sugars from cellulosic biomass 有权用于从纤维素生物质生产可溶性糖的重组和葡糖苷酶变体公开(公告)号：US08685690B2
公开(公告)日：2014-04-01
申请号：US13416608
申请日：2012-03-09
申请人： Jie Yang , Xiyun Zhang , Attila Andor
发明人： Jie Yang , Xiyun Zhang , Attila Andor
CPC分类号： C12N9/42 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , Y02E50/16
摘要： The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.
摘要翻译： 本发明涉及真菌C1菌株和葡萄糖苷酶的变体形式的重组表达。 本发明还涉及通过使用表达“葡糖苷酶”变体的转基因生物体从生物质产生可发酵糖并通过发酵糖生产生物燃料。 本发明提供了通过将纤维二糖与重组体 - 葡糖苷酶变体蛋白（例如由重组宿主细胞分泌的变体蛋白）接触到培养基中来从纤维二糖生产可发酵糖例如葡萄糖的方法。 本发明的方法可用于将生物质底物转化为可发酵的糖，最终转化为乙醇或其他生物燃料。
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公开(公告)号：US20130095530A1
公开(公告)日：2013-04-18
申请号：US13719431
申请日：2012-12-19
申请人： Codexis, Inc.
IPC分类号： C12N9/42
CPC分类号： C12N9/42 , C12N9/2445 , C12P7/06 , C12P19/14 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The invention provides variants of the Azospirillum irakense CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
摘要翻译： 本发明提供了与野生型酶相比具有改善的β-葡糖苷酶活性，特别是改进的热活性的Azospirillum irakense CelAβ-葡糖苷酶的变体。 本发明还提供了相关的多核苷酸，载体，宿主细胞，以及用于制备和使用变体的方法。
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公开(公告)号：US08715995B2
公开(公告)日：2014-05-06
申请号：US13678647
申请日：2012-11-16
申请人： Novozymes A/S , Novozymes, Inc.
CPC分类号： C12N9/42 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12P19/14 , C12Y302/01021 , C12Y302/01037
摘要： The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
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9.发明授权Method of converting a biomass substrate to a fermentable sugar using a beta-glucosidase variant enzyme 有权使用β-葡糖苷酶变体酶将生物质底物转化为可发酵糖的方法公开(公告)号：US08530201B2
公开(公告)日：2013-09-10
申请号：US13719431
申请日：2012-12-19
申请人： Codexis, Inc.
CPC分类号： C12N9/42 , C12N9/2445 , C12P7/06 , C12P19/14 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The invention provides variants of the Azospirillum irakense CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
摘要翻译： 本发明提供了与野生型酶相比具有改善的β-葡糖苷酶活性，特别是改进的热活性的Azospirillum irakense CelAβ-葡糖苷酶的变体。 本发明还提供了相关的多核苷酸，载体，宿主细胞，以及用于制备和使用变体的方法。
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10.发明授权公开(公告)号：US08323947B2
公开(公告)日：2012-12-04
申请号：US13416555
申请日：2012-03-09
申请人： Jie Yang , Xiyun Zhang , Attila Andor
发明人： Jie Yang , Xiyun Zhang , Attila Andor
CPC分类号： C12N9/42 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , Y02E50/16
摘要： The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.
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