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公开(公告)号：US08357523B2
公开(公告)日：2013-01-22
申请号：US12816989
申请日：2010-06-16
IPC分类号： C12N9/26
CPC分类号： C12N9/42 , C12N9/2445 , C12P7/06 , C12P19/14 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The invention provides variants of the Azospirillum irakense CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
摘要翻译： 本发明提供了与野生型酶相比具有改善的β-葡糖苷酶活性，特别是改进的热活性的Azospirillum irakense CelA和葡糖苷酶的变体。 本发明还提供了相关的多核苷酸，载体，宿主细胞，以及用于制备和使用变体的方法。
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公开(公告)号：US08772010B2
公开(公告)日：2014-07-08
申请号：US13375189
申请日：2010-06-16
申请人： Xiyun Zhang , Jie Yang , Dipnath Baidyaroy , Sally Rhiannon Postlethwaite , Attila Andor , Louis Clark , Lisa M. Newman
发明人： Xiyun Zhang , Jie Yang , Dipnath Baidyaroy , Sally Rhiannon Postlethwaite , Attila Andor , Louis Clark , Lisa M. Newman
CPC分类号： C12N9/2445 , C12N9/2482 , C12Y302/01021 , Y02E50/17
摘要： The invention relates to recombinantly produced β-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant β-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target β-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
摘要翻译： 本发明涉及与天然存在的蛋白质相比具有增强的热活性的重组产生的和 - 葡萄糖苷酶变体。 本发明还提供用于通过鉴定靶标和葡糖苷酶多肽中的性能敏感位置并用热活性增强残基取代该位置处的残基来制备具有改进的热活性的变体和葡糖苷酶多肽的方法。
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公开(公告)号：US20120077216A1
公开(公告)日：2012-03-29
申请号：US13375189
申请日：2010-06-16
申请人： Xiyun Zhang , Jie Yang , Dipnath Baidyaroy , Sally Rhiannon Postlethwaite , Attila Andor , Louis Clark , Lisa M. Newman
发明人： Xiyun Zhang , Jie Yang , Dipnath Baidyaroy , Sally Rhiannon Postlethwaite , Attila Andor , Louis Clark , Lisa M. Newman
CPC分类号： C12N9/2445 , C12N9/2482 , C12Y302/01021 , Y02E50/17
摘要： The invention relates to recombinantly produced β-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant β-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target β-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
摘要翻译： 本发明涉及与天然存在的蛋白质相比具有增强的热活性的重组产生的和 - 葡萄糖苷酶变体。 本发明还提供用于通过鉴定靶标和葡糖苷酶多肽中的性能敏感位置并用热活性增强残基取代该位置处的残基来制备具有改进的热活性的变体和葡糖苷酶多肽的方法。
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公开(公告)号：US20100317059A1
公开(公告)日：2010-12-16
申请号：US12816989
申请日：2010-06-16
CPC分类号： C12N9/42 , C12N9/2445 , C12P7/06 , C12P19/14 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The invention provides variants of the Azospirillum irakense CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.
摘要翻译： 本发明提供了与野生型酶相比具有改善的β-葡糖苷酶活性，特别是改进的热活性的Azospirillum irakense CelA和葡糖苷酶的变体。 本发明还提供了相关的多核苷酸，载体，宿主细胞，以及用于制备和使用变体的方法。
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