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    Live attenuated antigenically marked classical swine fever vaccine
    8.
    发明授权
    Live attenuated antigenically marked classical swine fever vaccine 有权
    活减毒抗原标记的经典猪瘟疫苗

    公开(公告)号：US09352032B2

    公开(公告)日：2016-05-31

    申请号：US14200802

    申请日：2014-03-07

    申请人： The United States of America, as Represented by the Secretary of Agriculture ,  The University of Connecticut

    发明人： Manuel V. Borca ,  Guillermo R. Risatti ,  Lauren G. Holinka-Patterson

    IPC分类号： C12N7/00 ,  A61K39/12 ,  G01N33/569

    CPC分类号： A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C07K2319/40 ,  C07K2319/43 ,  C12N7/00 ,  C12N2770/24321 ,  C12N2770/24322 ,  C12N2770/24334 ,  C12N2770/24351 ,  C12N2770/24362 ,  C12N2770/24371 ,  G01N33/56983 ,  G01N2333/183

    摘要： Controlling Classical Swine Fever Virus (CSFV) involves either prophylactic vaccination or non-vaccination and elimination of infected herds depending on the epidemiological situation. Marker vaccines allowing distinction between naturally infected from vaccinated swine could complement “stamping out” measures. Previously, we reported the development of FlagT4v, a double antigenic marker live attenuated CSFV strain. FlagT4v was later shown as not to be completely stable in terms of its attenuation when assessed in a reversion to virulence protocol. We have developed a modified version of the FlagT4v where changes in the codon usage of genomic areas encoding for Flag and T4 were introduced to rectify the reversion to the virulent genotype. The new virus, FlagT4-mFT-Gv, possesses the same amino acid sequence as FlagT4v except for one substitution, Asparagine is replaced by Glycine at position 852 of the CSFV polypeptide. FlagT4-mFT-Gv protected swine against challenge with Brescia virulent virus at 21 days post vaccination.

    摘要翻译： 控制经典猪热病毒（CSFV）涉及预防性接种疫苗或非疫苗接种，并根据流行病学情况消除感染的畜群。 允许区分接种猪的天然感染的标记疫苗可以补充“冲洗”措施。 以前，我们报道了FlagT4v的发展，FlagT4v是一种双抗原标志物活减毒CSFV毒株。 FlagT4v后来被证明在评估为毒力方案的逆转时，其衰减方面不能完全稳定。 我们已经开发了FlagT4v的修改版本，其中引入了编码Flag和T4的基因组区域的密码子使用的变化来纠正到毒性基因型的逆转。 新病毒FlagT4-mFT-Gv具有与FlagT4v相同的氨基酸序列，除了一个取代之外，天冬酰胺被CSFV多肽的852位的甘氨酸取代。 FlagT4-mFT-Gv在接种后21天保护猪免受布雷西亚毒性病毒攻击。

    Live Attenuated Antigenically Marked Classical Swine Fever Vaccine
    9.
    发明申请
    Live Attenuated Antigenically Marked Classical Swine Fever Vaccine 有权
    生物减毒的抗原标记的经典猪热疫苗

    公开(公告)号：US20150250866A1

    公开(公告)日：2015-09-10

    申请号：US14200802

    申请日：2014-03-07

    申请人： The University of Connecticut ,  The United States of America, as Represented by the Secretary of Agriculture

    发明人： Manuel V. Borca ,  Guillermo R. Risatti ,  Lauren G. Holinka-Patterson

    IPC分类号： A61K39/12 ,  C12N5/071 ,  G01N33/569 ,  C12N7/00

    CPC分类号： A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C07K2319/40 ,  C07K2319/43 ,  C12N7/00 ,  C12N2770/24321 ,  C12N2770/24322 ,  C12N2770/24334 ,  C12N2770/24351 ,  C12N2770/24362 ,  C12N2770/24371 ,  G01N33/56983 ,  G01N2333/183

    摘要： Controlling Classical Swine Fever Virus (CSFV) involves either prophylactic vaccination or non-vaccination and elimination of infected herds depending on the epidemiological situation. Marker vaccines allowing distinction between naturally infected from vaccinated swine could complement “stamping out” measures. Previously, we reported the development of FlagT4v, a double antigenic marker live attenuated CSFV strain. FlagT4v was later shown as not to be completely stable in terms of its attenuation when assessed in a reversion to virulence protocol. We have developed a modified version of the FlagT4v where changes in the codon usage of genomic areas encoding for Flag and T4 were introduced to rectify the reversion to the virulent genotype. The new virus, FlagT4-mFT-Gv, possesses the same amino acid sequence as FlagT4v except for one substitution, Asparagine is replaced by Glycine at position 852 of the CSFV polypeptide. FlagT4-mFT-Gv protected swine against challenge with Brescia virulent virus at 21 days post vaccination.

    摘要翻译： 控制经典猪热病毒（CSFV）涉及预防性接种疫苗或非疫苗接种，并根据流行病学情况消除感染的畜群。 允许区分接种猪的天然感染的标记疫苗可以补充“冲洗”措施。 以前，我们报道了FlagT4v的发展，FlagT4v是一种双抗原标志物活减毒CSFV毒株。 FlagT4v后来被证明在评估为毒力方案的逆转时，其衰减方面不能完全稳定。 我们已经开发了FlagT4v的修改版本，其中引入了编码Flag和T4的基因组区域的密码子使用的变化来纠正到毒性基因型的逆转。 新病毒FlagT4-mFT-Gv具有与FlagT4v相同的氨基酸序列，除了一个取代之外，天冬酰胺被CSFV多肽的852位的甘氨酸取代。 FlagT4-mFT-Gv在接种后21天保护猪免受布雷西亚毒性病毒攻击。