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    Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein
    1.
    发明授权
    Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein 有权
    通过使用具有改变的外壳蛋白的非哺乳动物DNA病毒在哺乳动物细胞中表达外源基因

    公开(公告)号：US06190887B1

    公开(公告)日：2001-02-20

    申请号：US09514953

    申请日：2000-02-28

    申请人： Frederick M. Boyce ,  James G. Barsoum

    发明人： Frederick M. Boyce ,  James G. Barsoum

    IPC分类号： C12P2104

    CPC分类号： C12N15/86 ,  A61K38/164 ,  A61K38/1758 ,  A61K38/191 ,  A61K38/45 ,  A61K38/50 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/14121 ,  C12N2710/14132 ,  C12N2710/14143 ,  C12N2710/14145 ,  C12N2760/20222 ,  C12N2810/6081

    摘要： Disclosed are methods, nucleic acids, and cells for expressing an exogenous gene in a mammalian cell, involving introducing into the cell a non-mammalian DNA virus (e.g., a baculovirus) having an altered coat protein, the genome of which virus carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed are methods for treating gene deficiency disorders, neurological disorders, or cancers in a mammal by (1) providing to a cell a therapeutically effective amount of a non-mammalian DNA virus having an altered coat protein, the genome of which virus carries an exogenous, therapeutic gene and (2) growing the cell under conditions such that the exogenous gene is expressed in the mammal.

    摘要翻译： 公开了用于在哺乳动物细胞中表达外源基因的方法，核酸和细胞，包括将具有改变的外壳蛋白的非哺乳动物DNA病毒（例如，杆状病毒）引入细胞，其病毒携带外源 基因，并在使基因表达的条件下生长细胞。 还公开了通过（1）向细胞提供治疗有效量的具有改变的外壳蛋白的非哺乳动物DNA病毒的方法，所述非哺乳动物DNA病毒具有改变的外壳蛋白，其病毒携带的基因组 外源性治疗基因和（2）在外源基因在哺乳动物中表达的条件下生长细胞。

    Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein
    4.
    发明授权
    Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein 失效
    通过使用具有改变的外壳蛋白的非哺乳动物DNA病毒在哺乳动物细胞中表达外源基因

    公开(公告)号：US06338953B1

    公开(公告)日：2002-01-15

    申请号：US08927317

    申请日：1997-09-11

    申请人： Frederick M. Boyce ,  James G. Barsoum

    发明人： Frederick M. Boyce ,  James G. Barsoum

    IPC分类号： C12P2104

    CPC分类号： C12N15/86 ,  A61K38/164 ,  A61K38/1758 ,  A61K38/191 ,  A61K38/45 ,  A61K38/50 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/14121 ,  C12N2710/14132 ,  C12N2710/14143 ,  C12N2710/14145 ,  C12N2760/20222 ,  C12N2810/6081

    摘要： Disclosed are methods, nucleic acids, and cells for expressing an exogenous gene in a mammalian cell, involving introducing into the cell a non-mammalian DNA virus (e.g., a baculovirus) having an altered coat protein, the genome of which virus carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed are methods for treating gene deficiency disorders, neurological disorders, or cancers in a mammal by (1) providing to a cell a therapeutically effective amount of a non-mammalian DNA virus having an altered coat protein, the genome of which virus carries an exogenous, therapeutic gene and (2) growing the cell under conditions such that the exogenous gene is expressed in the mammal.

    摘要翻译： 公开了用于在哺乳动物细胞中表达外源基因的方法，核酸和细胞，包括将具有改变的外壳蛋白的非哺乳动物DNA病毒（例如，杆状病毒）引入细胞，其病毒携带外源 基因，并在使基因表达的条件下生长细胞。 还公开了通过（1）向细胞提供治疗有效量的具有改变的外壳蛋白的非哺乳动物DNA病毒的方法，所述非哺乳动物DNA病毒具有改变的外壳蛋白，其病毒携带的基因组 外源性治疗基因和（2）在外源基因在哺乳动物中表达的条件下生长细胞。