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    Use of a baculovirus to express an exogenous gene in a mammalian cell
    1.
    发明授权
    Use of a baculovirus to express an exogenous gene in a mammalian cell 失效
    使用杆状病毒在哺乳动物细胞中表达外源基因

    公开(公告)号：US07192933B1

    公开(公告)日：2007-03-20

    申请号：US08752032

    申请日：1996-11-19

    申请人： Frederick M. Boyce

    发明人： Frederick M. Boyce

    IPC分类号： A61K31/70 ,  C07H21/02 ,  C12N1/20 ,  C12N15/00 ,  C12N5/00

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/14143

    摘要： Disclosed is a method of expressing an exogenous gene in a mammalian cell, involving infecting the cell with a baculovirus whose genome carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed is a method of treating a gene deficiency disorder in a mammal by providing to a cell a therapeutically effective amount of a baculovirus whose genome carries an exogenous gene and growing the cell under conditions such that the exogenous gene is expressed in the mammal.

    摘要翻译： 公开了一种在哺乳动物细胞中表达外源基因的方法，包括用基因组携带外源基因的杆状病毒感染细胞，并在使基因表达的条件下生长细胞。 还公开了通过向细胞提供治疗有效量的基因组携带外源基因并在使外源基因在哺乳动物中表达的条件下生长细胞的杆状病毒来治疗哺乳动物中的基因缺陷障碍的方法。

    Non-mammalian DNA virus to express an exogenous gene in a mammalian cell
    3.
    发明授权
    Non-mammalian DNA virus to express an exogenous gene in a mammalian cell 失效
    非哺乳动物DNA病毒在哺乳动物细胞中表达外源基因

    公开(公告)号：US5731182A

    公开(公告)日：1998-03-24

    申请号：US486341

    申请日：1995-06-07

    申请人： Frederick M. Boyce

    发明人： Frederick M. Boyce

    IPC分类号： A61K48/00 ,  C12N15/866 ,  C12N9/00 ,  C12N15/63 ,  C12P21/02

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/14143

    摘要： Disclosed is a method of expressing an exogenous gene in a mammalian cell, involving infecting the cell with a non-mammalian virus (e.g., a baculovirus) whose genome carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed is a method of treating a gene deficiency disorder in a mammal by providing to a cell a therapeutically effective amount of a virus whose genome carries an exogenous gene and growing the cell under conditions such that the exogenous gene is expressed in the mammal.

    摘要翻译： 公开了一种在哺乳动物细胞中表达外源基因的方法，包括用基因组携带外源基因的非哺乳动物病毒（例如杆状病毒）感染细胞，并在表达基因的条件下生长细胞。 还公开了通过向细胞提供治疗有效量的基因组携带外源基因并在使外源基因在哺乳动物中表达的条件下生长细胞的病毒的方法来治疗哺乳动物中的基因缺陷病症。

    Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein
    5.
    发明授权
    Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein 失效
    通过使用具有改变的外壳蛋白的非哺乳动物DNA病毒在哺乳动物细胞中表达外源基因

    公开(公告)号：US06338953B1

    公开(公告)日：2002-01-15

    申请号：US08927317

    申请日：1997-09-11

    申请人： Frederick M. Boyce ,  James G. Barsoum

    发明人： Frederick M. Boyce ,  James G. Barsoum

    IPC分类号： C12P2104

    CPC分类号： C12N15/86 ,  A61K38/164 ,  A61K38/1758 ,  A61K38/191 ,  A61K38/45 ,  A61K38/50 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/14121 ,  C12N2710/14132 ,  C12N2710/14143 ,  C12N2710/14145 ,  C12N2760/20222 ,  C12N2810/6081

    摘要： Disclosed are methods, nucleic acids, and cells for expressing an exogenous gene in a mammalian cell, involving introducing into the cell a non-mammalian DNA virus (e.g., a baculovirus) having an altered coat protein, the genome of which virus carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed are methods for treating gene deficiency disorders, neurological disorders, or cancers in a mammal by (1) providing to a cell a therapeutically effective amount of a non-mammalian DNA virus having an altered coat protein, the genome of which virus carries an exogenous, therapeutic gene and (2) growing the cell under conditions such that the exogenous gene is expressed in the mammal.

    摘要翻译： 公开了用于在哺乳动物细胞中表达外源基因的方法，核酸和细胞，包括将具有改变的外壳蛋白的非哺乳动物DNA病毒（例如，杆状病毒）引入细胞，其病毒携带外源 基因，并在使基因表达的条件下生长细胞。 还公开了通过（1）向细胞提供治疗有效量的具有改变的外壳蛋白的非哺乳动物DNA病毒的方法，所述非哺乳动物DNA病毒具有改变的外壳蛋白，其病毒携带的基因组 外源性治疗基因和（2）在外源基因在哺乳动物中表达的条件下生长细胞。

    Use of a non-mammalian DNA virus to express an exogenous gene in a mammalian cell
    6.
    发明授权
    Use of a non-mammalian DNA virus to express an exogenous gene in a mammalian cell 失效
    使用非哺乳动物DNA病毒在哺乳动物细胞中表达外源基因

    公开(公告)号：US06238914B1

    公开(公告)日：2001-05-29

    申请号：US08752030

    申请日：1996-11-19

    申请人： Frederick M. Boyce

    发明人： Frederick M. Boyce

    IPC分类号： C12N1563

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/14143

    摘要： Disclosed is a method of expressing an exogenous gene in a mammalian cell, involving infecting the cell with a non-mammalian virus (e.g., a baculovirus) whose genome carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed is a method of treating a gene deficiency disorder in a mammal by providing to a cell a therapeutically effective amount of a virus whose genome carries an exogenous gene and growing the cell under conditions such that the exogenous gene is expressed in the mammal.

    摘要翻译： 公开了一种在哺乳动物细胞中表达外源基因的方法，包括用基因组携带外源基因的非哺乳动物病毒（例如杆状病毒）感染细胞，并在表达基因的条件下生长细胞。 还公开了通过向细胞提供治疗有效量的基因组携带外源基因并在使外源基因在哺乳动物中表达的条件下生长细胞的病毒的方法来治疗哺乳动物中的基因缺陷病症。

    COMPOSITIONS AND METHODS FOR NUCLEIC ACID DELIVERY
    7.
    发明申请
    COMPOSITIONS AND METHODS FOR NUCLEIC ACID DELIVERY 审中-公开
    用于核酸递送的组合物和方法

    公开(公告)号：US20110111459A1

    公开(公告)日：2011-05-12

    申请号：US12936555

    申请日：2009-04-20

    申请人： Frederick M. Boyce

    发明人： Frederick M. Boyce

    IPC分类号： C12P21/02 ,  C12N5/071 ,  C12N15/85

    CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N15/88 ,  C12N2710/14022 ,  C12N2710/14023

    摘要： Disclosed herein are methods and compositions for preparing a lipid encapsulated nucleocapsid delivery composition capable of delivering a nucleic acid to a mammalian cell. The nucleocapsid delivery compositions disclosed herein are useful for large-scale protein production, expression of genes in a mammalian expression system, and/or pharmaceutical production of a recombinant protein for treatment of an individual. In addition, the nucleocapsid delivery compositions can be used to treat an individual to replace a gene or gene product, or alternatively to inhibit a gene product using an RNA interference molecule.

    摘要翻译： 本文公开了用于制备能够将核酸递送至哺乳动物细胞的脂质包封的核衣壳递送组合物的方法和组合物。 本文公开的核衣壳递送组合物可用于大规模蛋白质生产，哺乳动物表达系统中基因的表达和/或用于个体治疗的重组蛋白质的药物生产。 此外，核衣壳递送组合物可以用于治疗个体来代替基因或基因产物，或者可以使用RNA干扰分子来抑制基因产物。

    Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein
    8.
    发明授权
    Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein 有权
    通过使用具有改变的外壳蛋白的非哺乳动物DNA病毒在哺乳动物细胞中表达外源基因

    公开(公告)号：US06190887B1

    公开(公告)日：2001-02-20

    申请号：US09514953

    申请日：2000-02-28

    申请人： Frederick M. Boyce ,  James G. Barsoum

    发明人： Frederick M. Boyce ,  James G. Barsoum

    IPC分类号： C12P2104

    CPC分类号： C12N15/86 ,  A61K38/164 ,  A61K38/1758 ,  A61K38/191 ,  A61K38/45 ,  A61K38/50 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/14121 ,  C12N2710/14132 ,  C12N2710/14143 ,  C12N2710/14145 ,  C12N2760/20222 ,  C12N2810/6081

    摘要： Disclosed are methods, nucleic acids, and cells for expressing an exogenous gene in a mammalian cell, involving introducing into the cell a non-mammalian DNA virus (e.g., a baculovirus) having an altered coat protein, the genome of which virus carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed are methods for treating gene deficiency disorders, neurological disorders, or cancers in a mammal by (1) providing to a cell a therapeutically effective amount of a non-mammalian DNA virus having an altered coat protein, the genome of which virus carries an exogenous, therapeutic gene and (2) growing the cell under conditions such that the exogenous gene is expressed in the mammal.

    摘要翻译： 公开了用于在哺乳动物细胞中表达外源基因的方法，核酸和细胞，包括将具有改变的外壳蛋白的非哺乳动物DNA病毒（例如，杆状病毒）引入细胞，其病毒携带外源 基因，并在使基因表达的条件下生长细胞。 还公开了通过（1）向细胞提供治疗有效量的具有改变的外壳蛋白的非哺乳动物DNA病毒的方法，所述非哺乳动物DNA病毒具有改变的外壳蛋白，其病毒携带的基因组 外源性治疗基因和（2）在外源基因在哺乳动物中表达的条件下生长细胞。

    Use of a baculovirus to express and exogenous gene in a mammalian cell
    10.
    发明授权
    Use of a baculovirus to express and exogenous gene in a mammalian cell 失效
    使用杆状病毒在哺乳动物细胞中表达和外源基因

    公开(公告)号：US5871986A

    公开(公告)日：1999-02-16

    申请号：US311157

    申请日：1994-09-23

    申请人： Frederick M. Boyce

    发明人： Frederick M. Boyce

    IPC分类号： A61K48/00 ,  C12N15/866 ,  C12N9/00 ,  C07H21/04 ,  C12N5/10 ,  C12N15/63

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/14143

    摘要： Disclosed is a method of expressing an exogenous gene in a mammalian cell, involving infecting the cell with a baculovirus whose genome carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed is a method of treating a gene deficiency disorder in a mammal by providing to a cell a therapeutically effective amount of a baculovirus whose genome carries an exogenous gene and growing the cell under conditions such that the exogenous gene is expressed in the mammal.

    摘要翻译： 公开了一种在哺乳动物细胞中表达外源基因的方法，包括用基因组携带外源基因的杆状病毒感染细胞，并在使基因表达的条件下生长细胞。 还公开了通过向细胞提供治疗有效量的基因组携带外源基因并在使外源基因在哺乳动物中表达的条件下生长细胞的杆状病毒来治疗哺乳动物中的基因缺陷障碍的方法。