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公开(公告)号：US12104191B2
公开(公告)日：2024-10-01
申请号：US17823853
申请日：2022-08-31
发明人： Yaping Xue , Shuping Zou , Zhentao Jiang , Jianmiao Xu , Feng Cheng , Yuguo Zheng
IPC分类号： C12P13/00 , C07K14/245 , C12N1/21 , C12N9/00 , C12N9/04 , C12N9/06 , C12N9/10 , C12N9/12 , C12N15/10 , C12N15/52 , C12N15/70
CPC分类号： C12P13/001 , C07K14/245 , C12N9/0006 , C12N9/0016 , C12N9/1077 , C12N9/1205 , C12N9/1241 , C12N9/93 , C12N15/1082 , C12N15/52 , C12N15/70 , C12Y101/01119 , C12Y104/01002 , C12Y204/02011 , C12Y207/01086 , C12Y207/07018 , C12Y603/01005 , C12N2800/101
摘要： A cofactor self-sufficient Escherichia coli and its construction method and application in the synthesis of L-glufosinate are provided. The present invention expresses a NADH kinase and key enzymes of the cofactor synthesis pathway in E. coli, and knocks out the genes of enzymes that catabolizes cofactor, and with the addition of co-metabolic intermediates during cell incubation, the intracellular NADP(H) concentration is increased by at least 50% and the catalytic activity of glufosinate dehydrogenase by 2-fold, resulting in a significant increase in the spatiotemporal yield of the glufosinate synthesis reaction.
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2.发明公开公开(公告)号：US20240239825A1
公开(公告)日：2024-07-18
申请号：US18555428
申请日：2022-04-22
发明人： Guijie LI , Yuanbin SHE , Jianbin ZHENG , Kewei XU
CPC分类号： C07F15/0086 , C09K11/06 , H10K50/11 , H10K50/15 , H10K50/16 , H10K50/181 , H10K85/346 , C09K2211/185
摘要： The present invention provides a high radiation rate platinum complex based on 1,8-substituted carbazole and an application thereof. The luminescent material of the present invention can efficiently control the excited state properties of material molecules by introducing substituents at 1-, 8-position of carbazole, which increases components of metal-to-ligand charge transfer state (MLCT), and improves the intersystem crossing rate of molecules; at the same time, the introduction of substituents at the 1-, 8-position of carbazole can increase the dihedral angle thereof with a pyridine ring, which can improve the rigidity of molecules, effectively reduce the energy consumed by the vibration and rotation of the carbazole ring in the molecules, and reduce non-radiative attenuation. The described factors can jointly increase the radiation rate of material molecules and shorten the lifetime of the excited state.
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公开(公告)号：US12031160B2
公开(公告)日：2024-07-09
申请号：US17419048
申请日：2019-06-28
发明人： Yaping Xue , Feng Cheng , Qinghua Li , Yuguo Zheng , Jianmiao Xu
CPC分类号： C12N9/0016 , C12P13/04
摘要： The present invention provides a glufosinate-ammonium dehydrogenase mutant and application in synthesis of L-glufosinate-ammonium thereof, the method uses 2-carbonyl-4-[(hydroxy)(methyl)phosphinoyl]-butyric acid or its salts as a substrate and the glufosinate-ammonium dehydrogenase or cells containing the glufosinate-ammonium dehydrogenase as a biocatalyst to carry out reductive amination, thereby obtaining L-glufosinate-ammonium. The method has features of high conversion rate of raw materials, high yield, easy separation and purification of the product, and high chiral purity; compared with other catalytic processes, the method in the present invention has features of relatively simple process and a conversion rate of raw materials up to 100%.
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公开(公告)号：US20240207810A1
公开(公告)日：2024-06-27
申请号：US18357170
申请日：2023-07-24
发明人： Yong NIE , Xiaojiang LIANG , Zhenyu WU , Jianan LUO , Jiawei MA , Wenhao Li
IPC分类号： B01J19/18
CPC分类号： B01J19/1825 , B01J2219/00094
摘要： A gas-liquid reactor is provided. The gas-liquid reactor includes a reactor housing, where an outer side of the reactor housing is provided with a heat exchange jacket device, and the reactor housing is provided with a liquid phase inlet, a gas phase inlet and a gas-liquid phase outlet, and is internally provided with an electric dispersion gas distributor in communication with the gas phase inlet, and the electric dispersion gas distributor is provided with needle electrodes; and row tubes are arranged above the electric dispersion gas distributor, lower ends of the row tubes are grounded, and upper ends of the row tubes are located below the gas-liquid phase outlet. The gas-liquid reactor is configured to continuously produce gas-liquid reaction, and a gas-phase material is dispersed by utilizing the needle electrodes to form micrometer-scaled bubbles to be premixed with a liquid-phase material, and then stably flows through the row tubes.
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5.发明公开公开(公告)号：US20240167634A1
公开(公告)日：2024-05-23
申请号：US18372564
申请日：2023-09-25
发明人： Yi MA , Chuanming LI , Xiaoling DENG , Xudong PENG , Xiangkai MENG , Jinbo JIANG
IPC分类号： F17C13/04
CPC分类号： F17C13/04 , F17C2203/0604 , F17C2203/0619 , F17C2203/066 , F17C2203/0663 , F17C2205/0323 , F17C2205/0394 , F17C2221/012
摘要： A bi-directional penetrating metal bottleneck structure of a type IV hydrogen storage tank is provided, which includes a metal valve seat, a plastic liner, and a fiber winding layer. A valve seat hole is formed on an inner side of the metal valve seat, and the metal valve seat includes an annular flange, a valve seat column section, and a valve seat extension. L-shaped through holes and radial penetrating holes are formed in the valve seat column section. The valve seat hole is an irregular through hole and is provided with an annular groove. The valve seat extension is provided with a winding inclined plane, a self-tightening conical surface, a circumferential groove, and axial penetrating holes. The plastic liner includes an inner plastic joint, an outer plastic joint, a tapered connecting section, L-shaped connectors, radial connectors, axial connectors, and a reinforcement section. A flared annular boss at an upper part of the inner plastic joint is tightly clamped and embedded in the annular groove, and a lower part of the inner plastic joint, the L-shaped connector and the radial connector enclose a “B”-shaped binding structure to wrap a lower part of the valve seat column section inside and outside. According to the present disclosure, binding fastening is achieved, a sealing effect is enhanced, and the reliability and stability of a connection between the plastic liner and the metal valve seat are ensured.
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6.发明公开公开(公告)号：US20240166017A1
公开(公告)日：2024-05-23
申请号：US18087814
申请日：2022-12-23
发明人： Xi Shen , Jiangping Gu , Yuejin Huang , Huaqiang Jin , Zhe Sun , Jihui Zheng , Kang Li , Ling Shi , Wei Hang , Hongyu Chen
CPC分类号： B60H1/00585 , G01M99/007
摘要： Disclosed is a testing device and method for a service life of an air-conditioning compressor of a new energy vehicle running with pure gas. The testing device includes an alternative refrigeration system, a measurement and control system, and a testing environment room. A main refrigeration cycle system of the alternative refrigeration system runs with pure gas, gas of a testing compressor is discharged via a discharge throttle valve, and then enters a suction end via a suction throttle valve and an electronic expansion valve, and since there is no phase change, a heat exchange amount is small; adjustable ranges of the discharge throttle valve, the suction throttle valve and the electronic expansion valve are wide; and based on the system structure, a small refrigeration system can be used to test a compressor having high cooling capacity, and the system can quickly achieve a target working condition with a small amount of refrigerant.
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公开(公告)号：US11879869B2
公开(公告)日：2024-01-23
申请号：US18352263
申请日：2023-07-14
发明人： Cong Ding , Shiqing Feng , Zhongyu Piao , Zhipeng Yuan , Jing Liu
摘要： Disclosed is a method for predicting surface quality of a burnishing workpiece. The method includes the steps: using vibration sensors and signal acquisition instrument to acquire vibration signals generated on a surface of the burnishing workpiece during machining, evaluating the surface quality of the burnishing workpiece based on a coupling coordination degree model, processing signals by using an ensemble empirical mode decomposition method, identifying power spectral density, kurtosis and form factor as signal characteristics, identifying a support vector machine as a decision-making model, optimizing penalty parameters and kernel function parameters by using the Bayesian optimization method, and establishing the relationship between the signal characteristics and the surface quality. The method can quickly identify the signal characteristics for evaluating the workpiece surface quality, thereby improving the workpiece surface quality by intervening in process parameters, making up for the technical defect that condition monitoring cannot be performed during the machining process.
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公开(公告)号：US20240002248A1
公开(公告)日：2024-01-04
申请号：US17958743
申请日：2022-10-03
发明人： Jia ZHAO , Xiaonian LI , Yuxue YUE , Saisai WANG , Yihan ZHU
CPC分类号： C01G3/006 , C01G3/00 , B22F9/04 , C01P2006/12 , B22F2009/043
摘要： The invention discloses a porous intermetallic compound and preparation method and application thereof. The pore structure of the porous intermetallic compound includes micropores and mesopores, and the micropores and mesopores are distributed in disorder, wherein the content of the micropores accounts for 6-68%, and the content of mesopores accounts for 32-92%; the specific surface area of the porous intermetallic compound is 50-1600 m2/g, and the porous intermetallic compound is a porous copper silicide intermetallic compound or porous copper-chalcogen intermetallic compound. The invention provides preparation methods of the porous intermetallic compound, and also provides an application of the porous intermetallic compound as a catalyst in the reaction of acetylene hydrochlorination to synthesize vinyl chloride. The porous intermetallic compound catalyst prepared by the invention can carry out the acetylene hydrochlorination reaction in a wide space velocity range, and has good catalytic activity.
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公开(公告)号：US20230111631A1
公开(公告)日：2023-04-13
申请号：US17910787
申请日：2020-12-26
发明人： YAPING XUE , FENG CHENG , CHENGHAO CAO , YUGUO ZHENG , SHUPING ZOU , JIANMIAO XU
摘要： The present invention discloses a method for preparing L-glufosinate ammonium by biological enzymatic de-racemization, a glufosinate ammonium dehydrogenase mutant and a use thereof. The method for preparing L-glufosinate ammonium by biological enzymatic de-racemization includes catalyzing D,L-glufosinate ammonium as a raw material by a multi-enzyme catalysis system to obtain L-glufosinate ammonium. The enzyme catalysis system includes D-amino acid oxidase for catalyzing D-glufosinate ammonium in the D,L-glufosinate ammonium to 2-carbonyl-4-[hydroxy(methyl)phosphonyl]butanoic acid, and a glufosinate ammonium dehydrogenase mutant for catalytically reducing 2-carbonyl-4-[hydroxy(methyl)phosphonyl]butanoic acid to L-glufosinate ammonium. The glufosinate ammonium dehydrogenase mutant is obtained by mutation of glufosinate-ammonium dehydrogenase in wild fungi Thiopseudomonas denitrificans at a mutation site of V377S. The glufosinate ammonium dehydrogenase mutant in the present invention has better catalytic efficiency. When racemic D, L-glufosinate ammonium is used as a substrate for a catalytic reaction, the conversion rate is much higher than the conversion rate of a wild-type enzyme, and the yield of 2-carbonyl-4-[hydroxy(methyl)phosphonyl]butanoic acid (PPO for short) is also greatly improved.
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10.发明申请公开(公告)号：US20220396816A1
公开(公告)日：2022-12-15
申请号：US17778627
申请日：2021-01-21
申请人： ZHEJIANG YONGTAI TECHNOLOGY CO., LTD. , ZHEJIANG UNIVERSITY OF TECHNOLOGY , ZHEJIANG YONGTAI PHARMACEUTICAL CO., LTD.
发明人： Zhiqiang LIU , Feng CHENG , Xiaojian ZHANG , Dongxu JIA , Yuguo ZHENG , Renbao HE , Yizhong JIN , Hongming SHAO , Jiaohua LIN , Feng ZHANG
摘要： The present invention discloses a transaminase mutant and application thereof in preparation of sitagliptin intermediates, the transaminase mutant is obtained by substitution of tyrosine with proline at position 74, substitution of glutamic acid with aspartic acid at position 228, substitution of leucine with alanine at position 254 and substitution of methionine with threonine at position 290 of the amino acid sequence shown in SEQ ID NO: 2. The present invention uses wet cells or a purified transaminase as a biocatalyst and a sitagliptin precursor ketone or a prochiral carbonyl compound as a substrate to prepare a sitagliptin intermediate or a sitagliptin ester intermediate; the total yield of the method reaches about 82%, and e.e. value of the product reaches 99%.
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