公开(公告)号：US12042531B2

公开(公告)日：2024-07-23

申请号：US17451607

申请日：2021-10-20

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Mary L. Disis ,  Denise Cecil ,  Meredith Slota

IPC分类号： C12N15/00 ,  A61K39/00 ,  C07K14/47 ,  C07K14/705 ,  C07K14/71 ,  C12N9/10 ,  C12N15/62 ,  A61K48/00

CPC分类号： A61K39/0011 ,  A61K39/001103 ,  A61K39/001106 ,  A61K39/001129 ,  A61K39/00115 ,  A61K39/001152 ,  C07K14/4702 ,  C07K14/705 ,  C07K14/70567 ,  C07K14/70596 ,  C07K14/71 ,  C12N9/104 ,  C12N15/62 ,  C12Y203/02 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/572 ,  A61K48/00

摘要： The compositions described herein include an epitope of a peptide that may elicit an immune response in a subject following administration. The compositions may comprise nucleic acids. The compositions may comprise peptides. The methods described herein include administering a composition comprising an epitope of a peptide to a subject in need thereof.

公开(公告)号：US20240207378A1

公开(公告)日：2024-06-27

申请号：US18485536

申请日：2023-10-12

申请人： University of Washington through its Center for Commercialization

发明人： David W. Russell ,  Roli K. Hirata

IPC分类号： A61K39/00 ,  A61K35/12 ,  A61K35/28 ,  C07K14/74

CPC分类号： A61K39/0005 ,  A61K35/28 ,  C07K14/70539 ,  A61K35/12 ,  A61K2039/515 ,  C07K2319/00 ,  C12N2740/17043 ,  C12N2750/14143 ,  C12N2800/30

摘要： The invention provides isolated primate cells preferably human cells that comprise a genetically engineered disruption in a beta-2 microglobulin (B2M) gene, which results in deficiency in MHC class I expression and function. Also provided are the method of using the cells for transplantation and treating a disease condition.

公开(公告)号：US11993815B2

公开(公告)日：2024-05-28

申请号：US17392175

申请日：2021-08-02

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6876

CPC分类号： C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

摘要： Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20240084385A1

公开(公告)日：2024-03-14

申请号：US18465952

申请日：2023-09-12

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC分类号： C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

CPC分类号： C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

摘要： Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20240083953A1

公开(公告)日：2024-03-14

申请号：US18475950

申请日：2023-09-27

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Andre LIEBER ,  Hongjie WANG

IPC分类号： C07K14/005 ,  A61K47/42 ,  C07K7/06 ,  C12N5/077 ,  C12N7/00 ,  C12N15/10 ,  C12N15/86 ,  G01N33/94

CPC分类号： C07K14/005 ,  A61K47/42 ,  C07K7/06 ,  C12N5/0652 ,  C12N7/00 ,  C12N15/10 ,  C12N15/86 ,  G01N33/94 ,  A61K38/00 ,  C12N2710/10321 ,  C12N2710/10322

摘要： Disclosed are recombinant adenoviral compositions and methods for their use in treating disorders associated with epithelial tissues.

公开(公告)号：US20230203082A1

公开(公告)日：2023-06-29

申请号：US18112871

申请日：2023-02-22

申请人： University of Washington through its Center for Commercialization

发明人： Michael H. Gelb ,  Arun Babu Kumar ,  Frances Hocutt ,  Zdenek Spacil ,  Mariana Natali Barcenas Rodriguez ,  Frantisek Turecek ,  C. Ronald Scott

IPC分类号： C07H15/203 ,  C07H19/01

CPC分类号： C07H15/203 ,  C07H19/01 ,  G01N2333/924 ,  C12Q1/34

摘要： Reagents, methods, and kits for assaying enzymes associated with lysosomal storage diseases MPS-I, MPS-II, MPS-IIIA, MPS-IIIB, MPS-IVA, MPS-VI, and MPS VII.

公开(公告)号：US11618764B2

公开(公告)日：2023-04-04

申请号：US16224001

申请日：2018-12-18

申请人： University of Washington through its Center for Commercialization

发明人： Michael H. Gelb ,  Arun Babu Kumar ,  Frances Hocutt ,  Zdenek Spacil ,  Mariana Natali Barcenas Rodriguez ,  Frantisek Turecek ,  C. Ronald Scott

IPC分类号： C07H15/203 ,  C07H19/01 ,  C12Q1/34

摘要： Reagents, methods, and kits for assaying enzymes associated with lysosomal storage diseases MPS-I, MPS-II, MPS-IIIA, MPS-IIIB, MPS-IVA, MPS-VI, and MPS VII.

公开(公告)号：US11583299B1

公开(公告)日：2023-02-21

申请号：US16295607

申请日：2019-03-07

申请人： University of Washington through its Center for Commercialization

发明人： Adam D. Maxwell ,  Bryan W. Cunitz ,  Wayne Kreider ,  Oleg A. Sapozhnikov ,  Ryan S. Hsi ,  Michael R. Bailey

IPC分类号： A61B17/22

摘要： A method for attempting to fragment or comminute an object in a body using ultrasound includes producing a burst wave lithotripsy (BWL) waveform by a therapy transducer. The BWL waveform is configured to fragment or comminute the object. The BWL waveform includes a first burst of continuous ultrasound cycles and a second burst of continuous ultrasound cycles. A burst frequency corresponds to a frequency of repeating the bursts of the BWL waveform. The method also includes determining a cycle frequency f of the continuous ultrasound cycles within the first burst and the second burst based on a target fragment size D, where the cycle frequency is: f(MHz)=0.47/D(mm).

公开(公告)号：US11480575B2

公开(公告)日：2022-10-25

申请号：US16702176

申请日：2019-12-03

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Daniel T. Chiu ,  Mengxia Zhao ,  Wyatt Nelson ,  Perry G. Schiro

IPC分类号： G01N33/574 ,  B01L3/00 ,  G01N15/06 ,  G01N15/14 ,  G01N33/49 ,  G01N21/64 ,  G01N33/53 ,  G01N35/10 ,  G01N35/00 ,  G01N15/10

摘要： Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

公开(公告)号：US20220315918A1

公开(公告)日：2022-10-06

申请号：US17716539

申请日：2022-04-08

申请人： University of Washington Through Its Center for Commercialization

发明人： Jay Ashok Shendure ,  Jerrod Joseph Schwartz ,  Andrew Colin Adey ,  Cho li Lee ,  Joseph Brian Hiatt ,  Jacob Otto Kitzman ,  Akash Kumar

IPC分类号： C12N15/10

摘要： Contiguity information is important to achieving high-quality de novo assembly of mammalian genomes and the haplotype-resolved resequencing of human genomes. The methods described herein pursue cost-effective, massively parallel capture of contiguity information at different scales