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公开(公告)号：US20230146484A1
公开(公告)日：2023-05-11
申请号：US17981671
申请日：2022-11-07
申请人： MANUS BIO INC.
IPC分类号： C12P19/56
CPC分类号： C12P19/56 , C12N1/205 , C12Y114/14 , C12Y114/13079 , C12Y204/01 , C12Y402/03019
摘要： The present invention provides engineered cells and methods for making high purity steviol glycosides, including RebM. In some aspects, the present invention provides host cells, such as bacterial cells (including but not limited to E. coli), that are engineered to overexpress and/or delete or inactivate one or more steviol glycoside transport proteins. The bacterial cells selectively export RebM, or other specific combination of steviol glycosides, out of the cell to increase productivity and reduce production costs associated with downstream purification. Non-target steviol glycosides are not transported to the extracellular medium in significant amounts.
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公开(公告)号：US11618908B2
公开(公告)日：2023-04-04
申请号：US17273567
申请日：2019-09-06
申请人： MANUS BIO INC.
发明人： Ryan N. Philippe , Ajikumar Parayil Kumaran , Christine Nicole S. Santos , Jason Donald , Stephen Sarria
摘要： The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
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公开(公告)号：US20220251607A1
公开(公告)日：2022-08-11
申请号：US17729096
申请日：2022-04-26
申请人： MANUS BIO INC.
发明人： Ajikumar Parayil KUMARAN , Ryan LIM , Jason DONALD , Hsien-Chung TSENG , Christine Nicole S. SANTOS , Ryan PHILIPPE
摘要： The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.
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公开(公告)号：US20220002764A1
公开(公告)日：2022-01-06
申请号：US17293230
申请日：2019-11-14
申请人： Manus Bio Inc.
IPC分类号： C12P7/42 , C12N9/12 , C12N15/52 , C12N1/16 , C12N1/32 , C12N1/20 , C12N9/02 , C12N9/10 , C12N9/88 , C12N9/00
摘要： Enzymes involved in cannabinoid biosynthesis are recombinantly expressed in a host cell. The host cell may be a prokaryote (e.g. Escherichia coli) or a eukaryote (e.g. Yarrowia lipolytica). The enzymes include a heterologous cannabigerolic acid synthase as well as additional enzymes involved in the biosynthesis of cannabinoid precursors such as geranyl diphosphate, olivetol, olivetolic acid, divarin and/or divarinic acid. Methods are provided for producing C5-cannabinoids and/or C3-cannabinoids by fermentation of the recombinant host cell. Alternatively, cannabinoids can be produced by biotransformation of cannabinoid precursors in recombinant cells or by disrupted recombinant cells.
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公开(公告)号：US20210254107A1
公开(公告)日：2021-08-19
申请号：US17273567
申请日：2019-09-06
申请人： MANUS BIO INC.
发明人： RYAN N. PHILIPPE , AJIKUMAR PARAYIL KUMARAN , CHRISTINE NICOLE S. SANTOS , JASON DONALD , STEPHEN SARRIA
摘要： The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
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公开(公告)号：US20210207078A1
公开(公告)日：2021-07-08
申请号：US17151871
申请日：2021-01-19
申请人： MANUS BIO, INC.
发明人： Aaron LOVE , Adel GHADERI , Jason Eric DONALD , Christine Nicole S. SANTOS , Ajikumar Parayil KUMARAN
摘要： Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.
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公开(公告)号：US10743567B2
公开(公告)日：2020-08-18
申请号：US16251993
申请日：2019-01-18
申请人： Manus Bio, Inc.
发明人： Ryan Philippe , Ajikumar Parayil Kumaran , Jason Donald , Krishna Patel , Swati Gupta , Ryan Lim , Liwei Li
摘要： The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
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公开(公告)号：US20240141392A1
公开(公告)日：2024-05-02
申请号：US18373659
申请日：2023-09-27
申请人： Givaudan SA , MANUS BIO INC.
发明人： Ajikumar Parayil Kumaran , Chin Giaw Lim , Liwei Li , Souvik Ghosh , Christopher Pirie , Anthony Qualley , Geoff Marshall-Hill , Martin Preininger
CPC分类号： C12P7/26 , A01N27/00 , A23L27/13 , C12N9/0073 , C12P5/002 , C12Y114/13078 , A23V2002/00 , C07K2319/03
摘要： The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
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公开(公告)号：US20240093258A1
公开(公告)日：2024-03-21
申请号：US18038067
申请日：2021-11-24
申请人： Manus Bio Inc.
发明人： Ajikumar PARAYIL KUMARAN , Christine Nicole S. SANTOS , Jason DONALD , Aaron LOVE , Yiying ZHENG , Adel GHADERI , Vineet SHASTRY , Lu CHEN , Christopher TOOMEY , Hannah LYNCH , Eric NIEMINEN
CPC分类号： C12P19/60 , C12N9/1051 , C12N9/90 , C12Y204/01017 , C12Y503/01009
摘要： In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
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公开(公告)号：US20230323314A1
公开(公告)日：2023-10-12
申请号：US18161938
申请日：2023-01-31
申请人： MANUS BIO INC.
发明人： Jason DONALD , Christopher PIRIE , Liwei LI , Huey-Ming MAK , Srishti TIBREWALA , Ajikumar PARAYIL KUMARAN
CPC分类号： C12N9/0079 , C12N9/00 , C12N15/52 , C12N15/70 , C12P5/007 , C12P19/56 , C07K2319/03
摘要： The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.
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