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    Process for purification of polypeptide using a buchner funnel
    3.
    发明授权
    Process for purification of polypeptide using a buchner funnel 失效
    使用布氏漏斗纯化多肽的方法

    公开(公告)号：US5612454A

    公开(公告)日：1997-03-18

    申请号：US776272

    申请日：1991-11-29

    申请人： Toshihiko Kaminuma ,  Toshii Iida ,  Masahiro Tajima

    发明人： Toshihiko Kaminuma ,  Toshii Iida ,  Masahiro Tajima

    IPC分类号： C07K1/16 ,  C07K1/36 ,  A61K38/00 ,  A61K38/02 ,  C07K1/00 ,  C07K5/00

    CPC分类号： C07K1/16 ,  C07K1/36

    摘要： An improved process for purifying a polypeptide using a packing material for reversed phase high performance liquid chromatography is provided. A process for purifying a polypeptide characterized in that an aqueous solution containing polypeptide obtained by pre-treating a polypeptide produced by a wide variety of cells to a predetermined state is adjusted to a specific pH value, to remove impurities, and is then treated with a packing material for reversed phase high performance liquid chromatography.

    摘要翻译： PCT No.PCT / JP91 / 00421 Sec。 371日期：1991年11月29日 102（e）1991年11月29日PCT PCT 1991年3月29日PCT。 公开号WO91 / 15502 日期1991年10月17日提供了一种使用反相高效液相色谱法的填充材料纯化多肽的改进方法。 一种纯化多肽的方法，其特征在于，将通过将由多种细胞产生的多肽预处理至预定状态而获得的含有多肽的水溶液调节至特定的pH值以除去杂质，然后用 用于反相高效液相色谱的包装材料。

    Phenylacetonitrile derivatives and hair tonics and external preparations for skin containing the derivatives
    5.
    发明申请
    Phenylacetonitrile derivatives and hair tonics and external preparations for skin containing the derivatives 审中-公开
    苯乙腈衍生物和头发滋养物以及含有衍生物的皮肤外用制剂

    公开(公告)号：US20050176822A1

    公开(公告)日：2005-08-11

    申请号：US10509246

    申请日：2003-03-28

    申请人： Naoto Hanyu ,  Koji Kobayashi ,  Masahiro Tajima ,  Akihiro Ishino

    发明人： Naoto Hanyu ,  Koji Kobayashi ,  Masahiro Tajima ,  Akihiro Ishino

    IPC分类号： A61K8/40 ,  A61K8/41 ,  A61P17/14 ,  A61Q7/00 ,  A61Q19/00 ,  C07C255/42 ,  C07C255/43 ,  A61K31/277

    CPC分类号： A61Q19/00 ,  A61K8/40 ,  A61K8/41 ,  A61Q7/00 ,  C07C255/42 ,  C07C255/43

    摘要： A hair growth promoting composition comprising, as an effective ingredient, a phenylacetonitrile derivative or a pharmacologically acceptable salt thereof expressed by the following Formula (I): wherein each of R1 and R2 is hydrogen atom, a C1-10 alkyl group, a C2-10 alkenyl group or C2-10 acyl group, or NR1R2 may be a heterocycle having 3-7 members; R3 is a C1-5 alkyl group; and each of R4, R5 and R6 is hydrogen atom or a C1-4 alkoxy group. The present invention provides a hair growth promoting composition having excellent effects on promoting hair regrowth, preventing or inhibiting hair loss, and the like in human by compounding above-specified phenylacetonitrile derivative as a effective ingredient thereto.

    摘要翻译： 一种毛发生长促进组合物，其包含由下式（I）表示的苯乙腈衍生物或其药理学上可接受的盐作为有效成分：其中R 1和R 2各自表示， SUP>是氢原子，C 1-10烷基，C 2-10链烯基或C 2-10酰基， 或NR 1 R 2可以是具有3-7个成员的杂环; R 3是C 1-5烷基; R 4，R 5和R 6中的每一个为氢原子或C 1-4烷氧基 。 本发明提供一种通过将上述规定的苯乙腈衍生物作为有效成分配合而具有对促进毛发再生，预防或抑制脱发等的效果的毛发生长促进组合物。

    Method of preparing an enzyme participating in C-terminal amidation
    6.
    发明授权
    Method of preparing an enzyme participating in C-terminal amidation 失效
    制备参与C-末端酰胺化的酶的方法

    公开(公告)号：US6156555A

    公开(公告)日：2000-12-05

    申请号：US172120

    申请日：1998-10-14

    申请人： Toshii Iida ,  Toshihiko Kaminuma ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto ,  Jiro Kishimoto ,  Ohji Ifuku ,  Ichiro Kato

    发明人： Toshii Iida ,  Toshihiko Kaminuma ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto ,  Jiro Kishimoto ,  Ohji Ifuku ,  Ichiro Kato

    IPC分类号： C07K1/107 ,  C12N9/02 ,  C12N15/53 ,  C12N15/60 ,  C12N9/48 ,  C12N9/14 ,  C12N9/78 ,  C12N9/80 ,  C12N15/00

    CPC分类号： C12N9/0071 ,  C07K1/107 ,  C12Y114/17003 ,  Y10S435/814 ,  Y10S435/815

    摘要： A purified enzyme-I is obtained that participates in C-terminal amidation by acting on a peptide C-terminal glycine adduct to form a peptide C-terminal .alpha.-hydroxyglycine adduct. The enzyme has an optimum pH of about 5 to 7, an optimum temperature of 25 to 40.degree. C. and a molecular weight of about 25 kDa or about 36 kDa, and metal ions and ascorbic acid act as a cofactor. A purified enzyme-II is obtained that participates in C-terminal amidation by acting on the peptide C-terminal .alpha.-hydroxyglycine adduct to produce a C-terminal amidated compound. The enzyme has an optimum pH of about 5 to 6, an optimum temperature of 15 to 35.degree. C. and a molecular weight of about 40 kDa or about 43 kDa. Enzyme-I does not act on the peptide C-terminal .alpha.-hydroxyglycine adduct and enzyme-II does not act on the peptide C-terminal glycine adduct. The enzymes may be purified from a biological material such as horse serum by affinity chromatography using a peptide C-terminal glycine adduct as a ligand. The enzymes may also be obtained from host cells transformed with a plasmid containing a cDNA coding for the enzymes. Assay of activity of the enzymes is carried out by measuring the C-terminal .alpha.-hydroxyglycine adduct or the C-terminal amidated compound that has been isolated such as by high performance liquid chromatography with the use of an acetonitrile-containing buffer.

    摘要翻译： 获得通过作用于肽C-末端甘氨酸加合物以形成肽C末端α-羟基甘氨酸加合物参与C-末端酰胺化的纯化的酶I. 酶的最佳pH为约5至7，最适温度为25至40℃，分子量为约25kDa或约36kDa，金属离子和抗坏血酸用作辅因子。 获得了通过作用于肽C-末端α-羟基甘氨酸加合物参与C-末端酰胺化产生C末端酰胺化合物的纯化的酶-II。 酶的最佳pH为约5至6，最适温度为15至35℃，分子量为约40kDa或约43kDa。 酶-I不对肽C-端α-羟基甘氨酸加合物起作用，酶-II不对肽C-末端甘氨酸加合物起作用。 可以使用肽C-末端甘氨酸加合物作为配体通过亲和层析从生物材料如马血清中纯化酶。 酶也可以从用含有编码酶的cDNA的质粒转化的宿主细胞获得。 酶的活性测定是通过使用含有乙腈的缓冲液，通过高分辨液相色谱法测定已分离的C末端α-羟基甘氨酸加成物或C末端酰胺化合物进行的。

    Hair revitalizing tonic composition containing a 2,2-dimethylpropanediol compound and use thereof
    10.
    发明授权
    Hair revitalizing tonic composition containing a 2,2-dimethylpropanediol compound and use thereof 失效
    包含2,2-二甲基丙二醇化合物的头发再生补品组合物及其应用

    公开(公告)号：US06177067B1

    公开(公告)日：2001-01-23

    申请号：US09169940

    申请日：1998-10-13

    申请人： Tsunao Magara ,  Yoshiharu Tsuji ,  Masahiro Tajima ,  Koji Kobayashi ,  Hirotada Fukunishi ,  Masazumi Watanabe

    发明人： Tsunao Magara ,  Yoshiharu Tsuji ,  Masahiro Tajima ,  Koji Kobayashi ,  Hirotada Fukunishi ,  Masazumi Watanabe

    IPC分类号： A61K706

    CPC分类号： A61K8/44 ,  A61K8/49 ,  A61K8/4926 ,  A61K8/494 ,  A61Q5/00 ,  A61Q5/006 ,  A61Q5/02 ,  A61Q7/00 ,  Y10S514/88

    摘要： Disclosed is a hair revitalizing tonic composition containing a dimethylpropanediol derivative of the formula: wherein R1 and R2 are each independently a C1-30 hydrocarbon group which may be substituted, or a five- or six-membered heterocyclic group which contains 1 to 4 heteroatoms selected from oxygen, sulfur and nitrogen atoms and which may be substituted, R3 is hydrogen atom, an alkyl group which may be substituted, an acyl group, an alkoxycarbonyl group, a phenoxycarbonyl group, or a carbamoyl group which may be substituted, and a and b are each 0 or 1, as well as a method for effecting hair growth promotion, hair growth stimulation, or hair loss prevention in mammals by using such a hair revitalizing tonic composition. Also disclosed is the use of a compound of formula (I) in the preparation of a hair revitalizing tonic composition. Thus, this invention can provide an excellent means of hair revitalization or hair loss prevention in mammals.

    摘要翻译： 本发明公开了一种含有下式的二甲基丙二醇衍生物的头发活力补品组合物：其中R 1和R 2各自独立地为可被取代的C 1-30烃基或选自含有1至4个杂原子的五元或六元杂环基 可以被氧，硫和氮原子取代，可以被取代，R3是氢原子，可被取代的烷基，酰基，烷氧基羰基，苯氧基羰基或可被取代的氨基甲酰基，以及 b各自为0或1，以及通过使用这种头发活化的滋补组合物来实现哺乳动物的头发生长促进，毛发生长刺激或脱发防止的方法。 还公开了式（I）化合物在制备头发活化滋补组合物中的用途。 因此，本发明可以提供哺乳动物的头发复兴或脱发预防的优良手段。