公开(公告)号：US20080113351A1

公开(公告)日：2008-05-15

申请号：US11598052

申请日：2006-11-13

申请人： Yuki Naito ,  Masato Fujino ,  Shinobu Oguchi ,  Yukikazu Natori

发明人： Yuki Naito ,  Masato Fujino ,  Shinobu Oguchi ,  Yukikazu Natori

IPC分类号： A61K48/00 ,  C12Q1/68 ,  C07H21/02

CPC分类号： C12N15/111 ,  A61K31/713 ,  A61K48/00 ,  C12N2310/14 ,  C12N2320/11 ,  C12N2330/30

摘要： The present invention provides a polynucleotide that not only has a high RNA interference effect on its target gene, but also has a very small risk of causing RNA interference against a gene unrelated to the target gene. A sequence segment conforming to the following rules (a) to (d) is searched from the base sequences of a target gene for RNA interference and, based on the search results, a polynucleotide capable of causing RNAi is designed, synthesized, etc.: (a) The 3′ end base is adenine, thymine, or uracil, (b) The 5′ end base is guanine or cytosine, (c) A 7-base sequence from the 3′ end is rich in one or more types of bases selected from the group consisting of adenine, thymine, and uracil, and (d) The number of bases is within a range that allows RNA interference to occur without causing cytotoxicity.

摘要翻译： 本发明提供了不仅对其靶基因具有高RNA干扰效应，而且对与靶基因无关的基因造成RNA干扰的风险很小的多核苷酸。 从用于RNA干扰的靶基因的碱基序列中搜索符合以下规则（a）至（d）的序列区段，并且基于搜索结果，设计，合成等能够引起RNAi的多核苷酸： （a）3'末端碱基是腺嘌呤，胸腺嘧啶或尿嘧啶，（b）5'末端碱基是鸟嘌呤或胞嘧啶，（c）3'末端的7碱基序列富含一种或多种 选自腺嘌呤，胸腺嘧啶和尿嘧啶的碱基，（d）碱基数在允许RNA干扰发生而不引起细胞毒性的范围内。

公开(公告)号：US20110054005A1

公开(公告)日：2011-03-03

申请号：US12662904

申请日：2010-05-11

申请人： Yuki Naito ,  Masato Fujino ,  Shinobu Oguchi ,  Yukikazu Natori

发明人： Yuki Naito ,  Masato Fujino ,  Shinobu Oguchi ,  Yukikazu Natori

IPC分类号： A61K31/713 ,  C07H21/00 ,  C12Q1/68 ,  C12N5/071 ,  A61P25/28 ,  A61P25/00 ,  A61P35/00 ,  A61P7/06 ,  A61P35/02 ,  A61P21/00 ,  A61P5/26

CPC分类号： C12N15/111 ,  A61K31/713 ,  A61K48/00 ,  C12N2310/14 ,  C12N2320/11 ,  C12N2330/30

摘要： The present invention provides a polynucleotide that not only has a high RNA interference effect on its target gene, but also has a very small risk of causing RNA interference against a gene unrelated to the target gene. A sequence segment conforming to the following rules (a) to (d) is searched from the base sequences of a target gene for RNA interference and, based on the search results, a polynucleotide capable of causing RNAi is designed, synthesized, etc.: (a) The 3′ end base is adenine, thymine, or uracil, (b) The 5′ end base is guanine or cytosine, (c) A 7-base sequence from the 3′ end is rich in one or more types of bases selected from the group consisting of adenine, thymine, and uracil, and (d) The number of bases is within a range that allows RNA interference to occur without causing cytotoxicity.

摘要翻译： 本发明提供了不仅对其靶基因具有高RNA干扰效应，而且对与靶基因无关的基因造成RNA干扰的风险很小的多核苷酸。 从用于RNA干扰的靶基因的碱基序列中搜索符合以下规则（a）至（d）的序列区段，并且基于搜索结果，设计，合成等能够引起RNAi的多核苷酸： （a）3'末端碱基是腺嘌呤，胸腺嘧啶或尿嘧啶，（b）5'末端碱基是鸟嘌呤或胞嘧啶，（c）3'末端的7碱基序列富含一种或多种 选自腺嘌呤，胸腺嘧啶和尿嘧啶的碱基，（d）碱基数在允许RNA干扰发生而不引起细胞毒性的范围内。

公开(公告)号：US20110033860A1

公开(公告)日：2011-02-10

申请号：US12908393

申请日：2010-10-20

申请人： Kaoru SAIGO ,  Kumiko Tei ,  Yuki Naito

发明人： Kaoru SAIGO ,  Kumiko Tei ,  Yuki Naito

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C07H21/02 ,  G06F19/00 ,  G06F17/30

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N15/1131 ,  C12N15/1138 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/11 ,  G16B30/00 ,  G16B50/00

摘要： In the present invention, a sequence segment conforming to the following rules (a) to (d) is searched from the base sequences of a target gene of RNA interference and, based on the search results, siRNA capable of causing RNAi is designed, synthesized: (a) The 3′ end base is adenine, thymine, or uracil, (b) The 5′ end base is guanine or cytosine, (c) A 7-base sequence from the 3′ end is rich in one or more types of bases selected from the group consisting of adenine, thymine, and uracil, and (d) The number of bases is within a range that allows RNA interference to occur without causing cytotoxicity.

摘要翻译： 在本发明中，从RNA干扰的靶基因的碱基序列中搜索符合以下规则（a）〜（d）的序列区段，根据搜索结果，设计能够引起RNAi的siRNA，合成 （a）3'末端碱基是腺嘌呤，胸腺嘧啶或尿嘧啶，（b）5'末端碱基是鸟嘌呤或胞嘧啶，（c）3'末端的7碱基序列富含一种或多种 的选自腺嘌呤，胸腺嘧啶和尿嘧啶的碱基，和（d）碱基数在允许RNA干扰发生而不引起细胞毒性的范围内。

公开(公告)号：US20070202504A1

公开(公告)日：2007-08-30

申请号：US10593753

申请日：2005-03-23

申请人： Shinichi Morishita ,  Tomoyuki Yamada ,  Yuki Naito

发明人： Shinichi Morishita ,  Tomoyuki Yamada ,  Yuki Naito

IPC分类号： C12Q1/68 ,  G06F17/30 ,  G06F19/00

CPC分类号： C12Q1/6869 ,  G16B25/00 ,  G16B30/00 ,  C12Q2525/149

摘要： It is intended to efficiently determine a base sequence specifically appearing in an expression gene. For this, providing that the expression gene consists of exons (301) . . . (306) and especially that exon (301) is united with exon (302) and exon (302) with exon (303), an aggregate of base sequences (401) (403) being a union of exon base sequences (301) . . . (305) and a boundary base sequence obtained by uniting together base sequences (404) and (405) and base sequences (406) and (407) respectively existing over boundaries between exon (301) and exon (302) and between exon (302) and exon (303) is formed, and the aggregate is searched. If a base sequence is one specifically appearing in the expression gene, the number of search results is 1 and otherwise, the number is plural.

摘要翻译： 旨在有效地确定特异性出现在表达基因中的碱基序列。 为此，提供表达基因由外显子（301）组成。 。 。 （306），特别是外显子（301）与外显子（302）和外显子（302）与外显子（303）结合，作为外显子碱基序列（301）的并集的碱基序列（401）（403）的聚集体。 。 。 （305）和通过将分别存在于外显子（301）和外显子（302）之间的边界上的基本序列（404）和（405）以及碱基序列（406）和（407）之间以及外显子（302）之间的组合而获得的边界碱基序列 ）和外显子（303），并搜索聚集体。 如果碱基序列是表达基因中特定出现的碱基序列，则搜索结果数为1，否则为数。

公开(公告)号：US20070166716A1

公开(公告)日：2007-07-19

申请号：US10579906

申请日：2004-11-18

申请人： Yuki Naito ,  Kumiko Tel ,  Shuhel Zenno ,  Kaoru Saigo

发明人： Yuki Naito ,  Kumiko Tel ,  Shuhel Zenno ,  Kaoru Saigo

IPC分类号： C12Q1/68

CPC分类号： C12N15/111 ,  C12N2310/14 ,  C12N2320/11 ,  C12Q1/68 ,  C12Q1/6897

摘要： The present invention provides methods whereby an RNAi activity and a miRNA activity can be evaluated simply and conveniently. The RNAi activity or the miRNA activity is evaluated by supplying a target-expressing molecule, which is a polynucleotide containing at least a target sequence and an expression regulatory region regulating the expression of an RNA containing the target sequence, and a subject nucleic acid molecule to be evaluated on whether or not it has RNAi activity on the RNA containing the target sequence into an expression system in which the target-expressing molecule can express the RNA containing the target sequence.

摘要翻译： 本发明提供了可以简单方便地评估RNAi活性和miRNA活性的方法。 通过提供靶向表达分子来评价RNAi活性或miRNA活性，所述分子是至少含有靶序列的多核苷酸和调节含有靶序列的RNA的表达的表达调节区和目标核酸分子， 评估其是否对含有靶序列的RNA具有RNAi活性，使其表达分子能够表达含有靶序列的RNA的表达系统。

公开(公告)号：US20100179875A1

公开(公告)日：2010-07-15

申请号：US12452502

申请日：2008-07-10

申请人： Yuki Naito ,  Genki Takiuchi

发明人： Yuki Naito ,  Genki Takiuchi

IPC分类号： G06Q30/00 ,  G06Q10/00 ,  G06F15/16

CPC分类号： G06Q30/02 ,  G06Q30/0255 ,  G06Q30/0256

摘要： Advertisement attribute information extracted in an advertisement distribution server (101) is transmitted to a viewing terminal (104), browser viewing history information and the viewing history candidate information that the advertiser has registered in association with each other in advance are matched when placing an advertisement, and the advertisement information included in the advertisement attribute information having the agreed viewing history URL as the viewing history candidate information is inserted into an advertising space on a Web page which is a medium. This, in providing Web advertising (Internet advertising), allows the advertiser to get higher advertising effectiveness, the medium to effectively use the advertising space, and the viewer to acquire an advertisement as useful information.

摘要翻译： 在广告分发服务器（101）中提取的广告属性信息被发送到观看终端（104），当放置广告时，浏览器观看历史信息和广告客户预先相关联登记的观看历史候选信息被匹配 并且包括在具有约定的观看历史URL的广告属性信息中的广告信息作为观看历史候选信息被插入作为媒体的网页上的广告空间中。 这在提供网络广告（因特网广告）时，允许广告商获得更高的广告效果，有效地使用广告空间的媒介，并且观众获取广告作为有用的信息。

公开(公告)号：US20060275762A1

公开(公告)日：2006-12-07

申请号：US10535851

申请日：2003-11-21

申请人： Kaoru Saigo ,  Kumiko Tei ,  Yuki Naito

发明人： Kaoru Saigo ,  Kumiko Tei ,  Yuki Naito

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N15/1131 ,  C12N15/1138 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/11 ,  G16B30/00 ,  G16B50/00

摘要： In the present invention, a sequence segment conforming to the following rules (a) to (d) is searched from the base sequences of a target gene of RNA interference and, based on the search results, siRNA capable of causing RNAi is designed, synthesized, etc.: (a) The 3′ end base is adenine, thymine, or uracil, (b) The 5′ end base is guanine or cytosine, (c) A 7-base sequence from the 3′ end is rich in one or more types of bases selected from the group consisting of adenine, thymine, and uracil, and (d) The number of bases is within a range that allows RNA interference to occur without causing cytotoxicity.

摘要翻译： 在本发明中，从RNA干扰的靶基因的碱基序列中搜索符合以下规则（a）〜（d）的序列区段，根据搜索结果，设计能够引起RNAi的siRNA，合成 （a）3'末端碱基是腺嘌呤，胸腺嘧啶或尿嘧啶，（b）5'末端碱基是鸟嘌呤或胞嘧啶，（c）3'端的7碱基序列富含1个 或更多种选自腺嘌呤，胸腺嘧啶和尿嘧啶的碱基，和（d）碱基数在允许RNA干扰发生而不引起细胞毒性的范围内。