公开(公告)号：US20120288959A1

公开(公告)日：2012-11-15

申请号：US13377146

申请日：2010-06-08

Applicant: Wesley Palmer ,  Michael Lee ,  Richard Young ,  Munisch Gupta

Inventor： Wesley Palmer ,  Michael Lee ,  Richard Young ,  Munisch Gupta

IPC: G01N33/566 ,  G01N21/78

CPC classification number: G01N21/78 ,  G01N33/54366 ,  G01N33/558 ,  G01N2333/4724 ,  G01N2333/79

Abstract: Methods and systems are disclosed for rapid detection of cerebrospinal fluid (CSF) in a sample. In some embodiments, the methods comprise depleting a biological sample of beta-1 transferrin by contacting the sample with a sialic acid-specific lectin bound to a solid support, followed by subjecting the beta-1 transferrin-depleted sample to a lateral flow immunoassay. The methods can be used to detect CSF comprised by a sample in under one hour. Furthermore, the methods can detect CSF in a biological sample such as a plasma sample of a volume as small as about 10 μl.

Abstract translation: 公开了用于在样品中快速检测脑脊液（CSF）的方法和系统。 在一些实施方案中，所述方法包括通过使样品与结合到固体支持物的唾液酸特异性凝集素接触，然后使β-1转铁蛋白贫化样品进行侧流免疫测定来消耗β-1转铁蛋白的生物样品。 该方法可用于在1小时内检测由样品组成的CSF。 此外，该方法可以检测生物样品中的CSF，例如体积小至约10μl的血浆样品。