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公开(公告)号：US20230131551A1
公开(公告)日：2023-04-27
申请号：US17668553
申请日：2022-02-10
Applicant: Wisconsin Alumni Research Foundation
Inventor： Hasan Khatib
IPC: C12Q1/6888
Abstract: Differentially expressed miRNA or small mRNA in the culture media of embryos were found to correlate to and affect embryo developmental fate. Accordingly the present invention provides a method for selecting a bovine embryo for implantation into a female bovine animal for further development based on the levels of specific miRNA or small mRNA. Also provided are methods of improving bovine embryo development fate by reducing in the culture medium the level of selected miRNAs.
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2.发明授权公开(公告)号：US09856531B2
公开(公告)日：2018-01-02
申请号：US14990810
申请日：2016-01-08
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Hasan Khatib
CPC classification number: C12Q1/6876 , A61D19/02 , A61D19/04 , C07H21/04 , C12Q1/6888 , C12Q2600/124 , C12Q2600/156 , C12Q2600/172
Abstract: An isolated nucleic acid molecule comprising a polymorphic site selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1, an array or a kit comprising the same. Also provided are a method for detecting single nucleotide polymorphism (SNP) in bovine proteinase inhibitor (PI) gene, a method for haplotyping a bovine cell, a method for progeny testing of cattle based on said haplotyping, a method for selectively breeding of cattle based on haplotyping a parent animal. The present invention further provides a method for testing a dairy cattle for its milk production trait, comprising haplotyping its cells, wherein a cattle having haplotypes 1, 3, 4 or 5 indicates that the cattle has desirable milk production trait. Haplotype 1 indicates that the cattle has the most desirable milk production trait.
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3.发明授权公开(公告)号：US09322068B2
公开(公告)日：2016-04-26
申请号：US14309868
申请日：2014-06-19
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Hasan Khatib
CPC classification number: C12Q1/6888 , A61D19/02 , A61D19/04 , C12Q1/6876 , C12Q2600/124 , C12Q2600/156
Abstract: Single nucleotide polymorphic sites of the bovine HSP genes are associated with improved fertilization rate and/or improved embryo survival rate in cattle. Nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods based on these SNPs are disclosed.
Abstract translation: 牛HSP基因的单核苷酸多态性位点与牛的改良受精率和/或改良的胚胎存活率有关。 公开了基于这些SNP的核酸分子，试剂盒，基因分型方法和标记辅助的牛育种方法。
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4.发明申请公开(公告)号：US20140330071A1
公开(公告)日：2014-11-06
申请号：US14309868
申请日：2014-06-19
Applicant: Wisconsin Alumni Research Foundation
Inventor： Hasan Khatib
CPC classification number: C12Q1/6888 , A61D19/02 , A61D19/04 , C12Q1/6876 , C12Q2600/124 , C12Q2600/156
Abstract: Single nucleotide polymorphic sites of the bovine HSP genes are associated with improved fertilization rate and/or improved embryo survival rate in cattle. Nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods based on these SNPs are disclosed.
Abstract translation: 牛HSP基因的单核苷酸多态性位点与牛的改良受精率和/或改良的胚胎存活率有关。 公开了基于这些SNP的核酸分子，试剂盒，基因分型方法和标记辅助的牛育种方法。
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公开(公告)号：US11155870B2
公开(公告)日：2021-10-26
申请号：US16371147
申请日：2019-04-01
Applicant: Wisconsin Alumni Research Foundation
Inventor： Hasan Khatib
IPC: C12Q1/6876 , C12N15/113
Abstract: Method for determining developmental fate of early embryos comprises measuring the expression level of a gene selected from the group consisting of CDKN1C, IGF2R, MAGEL2, MKRN3, NAP1L5, NDN, PEG3, PHLDA2, TSSC4, and UBE3A genes. Also disclosed is a method for improving pregnancy rate, wherein early embryos whose expression level of the MKRN3, NDN, PEG3, PHLDA2, TSSC4, or UBE3A gene is not increased, or the expression level of the CDKN1C, IGF2R, MAGEL2, or NAP1L5 gene is not decreased, are selected for planting into a suitable uterus for further development. Also disclosed are methods for increasing the likelihood of an early embryo to develop successfully into full-term pregnancy, wherein a suitable amount of siRNA corresponding to the PHLDA2 gene is injected into a fertilized egg which is in turn cultured further and planted into a suitable uterus.
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Patent Agency Ranking
公开(公告)号：US20190218622A1
公开(公告)日：2019-07-18
申请号：US16278723
申请日：2019-02-19
Applicant: Wisconsin Alumni Research Foundation
Inventor： Hasan Khatib
IPC: C12Q1/6888
CPC classification number: C12Q1/6888 , C12Q2600/124 , C12Q2600/158 , C12Q2600/178
Abstract: Differentially expressed miRNA or small mRNA in the culture media of embryos were found to correlate to and affect embryo developmental fate. Accordingly the present invention provides a method for selecting a bovine embryo for implantation into a female bovine animal for further development based on the levels of specific miRNA or small mRNA. Also provided are methods of improving bovine embryo development fate by reducing in the culture medium the level of selected miRNAs.
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公开(公告)号：US20190002992A9
公开(公告)日：2019-01-03
申请号：US15138221
申请日：2016-04-26
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Hasan Khatib
Abstract: Arrays of nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods based on novel SNPs on genes of the bovine transforming growth factor-β (TGF-β) signaling pathway for improved bovine fertilization rate. The methods and compositions of the present invention are related to SNPs in the DNA-binding protein inhibitor 3 (ID3) gene, and in the bone morphogenetic protein 4 (BMP4) gene corresponding to position 2702 of SEQ ID NO: 2. Also disclosed are methods for determining viability of developing bovine embryos by measuring the expression level of one or more target genes in the TGF-signaling pathway, and selecting for implantation only embryos whose target gene expression level is not up-regulated.
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公开(公告)号：US20160333427A1
公开(公告)日：2016-11-17
申请号：US15155068
申请日：2016-05-15
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Hasan Khatib
IPC: C12Q1/68
CPC classification number: C12Q1/6888 , C12Q2600/124 , C12Q2600/158 , C12Q2600/178
Abstract: Differentially expressed miRNA or small mRNA in the culture media of embryos were found to correlate to and affect embryo developmental fate. Accordingly the present invention provides a method for selecting a bovine embryo for implantation into a female bovine animal for further development based on the levels of specific miRNA or small mRNA. Also provided are methods of improving bovine embryo development fate by reducing in the culture medium the level of selected miRNAs.
Abstract translation: 胚胎培养基中差异表达的miRNA或小mRNA被发现与胚胎发育命运相关并影响胚胎发育命运。 因此，本发明提供了一种基于特异性miRNA或小mRNA水平，选择用于植入雌性牛动物进行进一步发育的牛胚胎的方法。 还提供了通过在培养基中减少所选择的miRNA的水平来改善牛胚胎发育命运的方法。
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公开(公告)号：US20140045711A1
公开(公告)日：2014-02-13
申请号：US13798181
申请日：2013-03-13
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Hasan Khatib
CPC classification number: C12Q1/6883 , A01K67/02 , A01K67/027 , A01K2227/101 , A01K2267/02 , A61D19/04 , A61K35/54 , C12Q1/68 , C12Q1/6888 , C12Q2600/124 , C12Q2600/156 , C12Q2600/16
Abstract: Single nucleotide polymorphic sites of the bovine MAP1B, PPP1R11, and DDX4 genes are associated with improved bull fertility as measured by e.g. sire conception rates. Nucleic acid molecules, arrays, kits, methods of genotyping and marker-assisted bovine breeding methods based on these SNPs are disclosed.
Abstract translation: 牛MAP1B，PPP1R11和DDX4基因的单核苷酸多态性位点与改良的公牛生育力相关联，如通过例如 父母受孕率。 公开了基于这些SNP的核酸分子，阵列，试剂盒，基因分型方法和标记辅助牛育种方法。
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10.发明授权公开(公告)号：US11680295B2
公开(公告)日：2023-06-20
申请号：US15345515
申请日：2016-11-08
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Hasan Khatib
IPC: C12Q1/6883
CPC classification number: C12Q1/6883 , C12Q2600/156 , C12Q2600/16
Abstract: Disclosed are arrays of nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods using SNPs on genes of the bovine interferon tau signaling pathway for improved bovine fertilization rate, or embryo survival, or both.
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