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公开(公告)号：US20220097042A1
公开(公告)日：2022-03-31
申请号：US17447015
申请日：2021-09-07
发明人： James P. Landers , Jacquelyn A. DuVall , Delphine Le Roux , Brian Root , Daniel Mills , Daniel A. Nelson , An-chi Tsuei , Brandon L. Thompson , Jingyi Li , Christopher Birch
摘要： A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.
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公开(公告)号：US20180304253A1
公开(公告)日：2018-10-25
申请号：US15768115
申请日：2016-10-13
发明人： James P. Landers , Jacquelyn A. DuVall , Delphine Le Roux , Brian Root , Daniel MIlls , Daniel A. Nelson , An-chi Tsuei , Brandon L. Thompson , Jingyi Li , Christopher Birch
IPC分类号： B01L3/00 , B01L7/00 , G01N27/447
CPC分类号： B01L3/50273 , B01L3/502753 , B01L7/52 , B01L2200/0621 , B01L2200/0647 , B01L2200/0684 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0803 , B01L2300/0816 , B01L2300/0887 , B01L2300/1822 , B01L2300/1844 , B01L2400/0409 , B01L2400/0421 , B01L2400/0424 , G01N27/447
摘要： A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.
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公开(公告)号：US11135583B2
公开(公告)日：2021-10-05
申请号：US15768115
申请日：2016-10-13
发明人： James P. Landers , Jacquelyn A. DuVall , Delphine Le Roux , Brian Root , Daniel Mills , Daniel A. Nelson , An-Chi Tsuei , Brandon L. Thompson , Jingyi Li , Christopher Birch
摘要： A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.
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