公开(公告)号：US11135583B2

公开(公告)日：2021-10-05

申请号：US15768115

申请日：2016-10-13

申请人： University of Virginia Patent Foundation

发明人： James P. Landers ,  Jacquelyn A. DuVall ,  Delphine Le Roux ,  Brian Root ,  Daniel Mills ,  Daniel A. Nelson ,  An-Chi Tsuei ,  Brandon L. Thompson ,  Jingyi Li ,  Christopher Birch

IPC分类号： B01L99/00 ,  B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  G01N21/07 ,  C12Q1/00 ,  G01N21/00

摘要： A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.