公开(公告)号：US20230313297A1

公开(公告)日：2023-10-05

申请号：US18308706

申请日：2023-04-28

申请人： Ultivue, Inc.

发明人： Abdul Majeed Mohammed ,  Mael Manesse ,  Mack J. Schermer ,  Mark N. Bobrow ,  Michael Jules Natan

IPC分类号： C12Q1/6876 ,  G01N21/76

CPC分类号： C12Q1/6876 ,  G01N21/76

摘要： Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.

公开(公告)号：US11754562B2

公开(公告)日：2023-09-12

申请号：US16992654

申请日：2020-08-13

申请人： Ultivue, Inc.

发明人： Stephanie Rae Hennek ,  Mael Manesse ,  Abdul Mohammed ,  Xi Chen ,  Michael Jules Natan

IPC分类号： C12Q1/6804 ,  C12Q1/6832 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6823 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/682

CPC分类号： G01N33/54386 ,  C12Q1/68 ,  C12Q1/682 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6832 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/54306 ,  G01N33/54333 ,  G01N33/58 ,  G01N33/587 ,  C12Q1/6816 ,  C12Q2531/125 ,  C12Q2537/143 ,  C12Q2563/179

摘要： The present application provides certain advantageous ways of conducting multiplexed imaging.

公开(公告)号：US11674178B2

公开(公告)日：2023-06-13

申请号：US16713760

申请日：2019-12-13

申请人： Ultivue, Inc.

发明人： Abdul Majeed Mohammed ,  Mael Manesse ,  Mack J. Schermer ,  Mark N. Bobrow ,  Michael Jules Natan

IPC分类号： C12Q1/6876 ,  G01N21/76

CPC分类号： C12Q1/6876 ,  G01N21/76

摘要： Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.