公开(公告)号：US20200222875A1

公开(公告)日：2020-07-16

申请号：US16726073

申请日：2019-12-23

Applicant: Twist Bioscience Corporation

Inventor： Bill James PECK ,  Mary NOE ,  Stefan PITSCH ,  Patrick A. Weiss

IPC: B01J19/00 ,  C07H21/04

Abstract: Methods, systems, compositions, and devices for the manufacturing of high-quality building blocks, such as polynucleotides, are described herein. Processes described herein provide for efficient washing of residual reagents, solvents, or byproducts from previous synthetic steps to allow for the generation of polynucleotides with low error rates. Processes described herein also provide for reduction in deletion rates during chemical nucleic acid synthesis. Further, methods and devices described herein allow for the rapid construction and assembly of large libraries of highly accurate polynucleotides.