公开(公告)号：US20250052744A1

公开(公告)日：2025-02-13

申请号：US18722913

申请日：2022-12-23

Applicant: The University of Liverpool

Inventor： Srijan Jindal

IPC: G01N33/53 ,  C09K11/06 ,  G01N33/569

Abstract: The present invention relates to a combination of fluorescent dyes for identifying, or differentiating between, different bacterial strains, wherein the combination comprises two or more of the following fluorescent dyes: SYBR Green I, SYTO 13 or 9, and/or DiSC3(5) and/or FM4-64. The device is particularly suited for identifying the type of bacterial infection causing a Urinary Tract Infection (UTI) and can be used to help identify which antimicrobial agents would be suitable for the treatment the infection.

公开(公告)号：US20250050326A1

公开(公告)日：2025-02-13

申请号：US18722887

申请日：2022-12-23

Applicant: The University of Liverpool

Inventor： Srijan Jindal ,  Douglas Kell

IPC: B01L3/00 ,  C12Q1/18

Abstract: The present invention relates to A device for performing an assay on an aqueous biological sample, the device comprising: (i) at least a first chamber comprising a desiccated hydrogel, wherein the hydrogel incorporates at least a first desiccated assay reagent or reagents, the hydrogel and reagent or reagents being configured to allow the controlled release of the first reagent or reagents when the hydrogel has been exposed to the aqueous biological sample and has at least been partially hydrated; and (ii) an imaging device for imaging and analysing the first chamber after the aqueous biological sample has been introduced into the first chamber and the hydrogel has been at least partially hydrated and at least part of the first reagent or reagents has or have been released. The present invention also relates to a device for rapidly determining the susceptibility of a microorganism in an aqueous biological sample to an antimicrobial agent comprising: a) a multi-chamber plate, wherein: (i) at least a first chamber comprises a growth medium, a first dye, and a desiccated hydrogel; and (ii) at least a second chamber comprises a growth medium, a first dye, a desiccated hydrogel, and a first antimicrobial agent that inhibits or slows the proliferation of the, or a, microorganism; and b) an imaging device for imaging and analysing one or more chambers after the biological sample has been introduced into the chamber and the hydrogel has been at least partially hydrated. The invention also relates to related methods and a kit of parts. The device is particularly suited for identifying the which antimicrobial agents would be suitable for the treatment of microbial infections, such as Urinary Tract Infections (UTIs).