公开(公告)号：US20180355322A1

公开(公告)日：2018-12-13

申请号：US16104533

申请日：2018-08-17

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Kei TSUKAMOTO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/09

CPC classification number: C12N5/0693 ,  C12M3/00 ,  C12N2501/91

Abstract: A method of promoting spheroid formation, including: a preparation step of preparing a mixture obtained by mixing a cell sample with a promoter; and a culture step of culturing, inside a spheroid formation-culture vessel, the mixture obtained in the preparation step, in which the promoter is a polymer in which one or more selected from the group consisting of D-glucosamine, D-galactosamine, D-glucuronic acid, L-iduronic acid, and D-galactose are polymerized.

公开(公告)号：US20210079375A1

公开(公告)日：2021-03-18

申请号：US16951456

申请日：2020-11-18

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Hiroki NAGASE ,  Kei TSUKAMOTO ,  Shiro KITANO

IPC: C12N15/10 ,  C07H1/06 ,  C07H21/00 ,  C08G69/10 ,  C08G73/06 ,  G01N1/40 ,  C12N15/09

Abstract: A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.

公开(公告)号：US20190049432A1

公开(公告)日：2019-02-14

申请号：US16164989

申请日：2018-10-19

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro KITANO ,  Kei TSUKAMOTO ,  Shinji IRIE

IPC: G01N33/50

Abstract: A method of assessing an anti-cancer drug including culturing a cell structure including cancer cells and stromal cells in a presence of at least one anti-cancer drug, and assessing an anti-cancer effect of the at least one anti-cancer drug based on a number of viable cancer cells in the cell structure after the culturing.

公开(公告)号：US20170029808A1

公开(公告)日：2017-02-02

申请号：US15292880

申请日：2016-10-13

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Kei TSUKAMOTO

IPC: C12N15/10

CPC classification number: C12N15/1006 ,  C07H1/08 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q2523/308

Abstract: A method of recovering short-chain nucleic acids is provided that includes: a step (a) of bringing a mixture having an alcohol concentration of 0 to 5 volume %, the mixture being prepared by mixing a whole blood sample with a first buffer containing one or more members selected from the group consisting of a chaotropic agent and a surfactant, into contact with a first nucleic acid adsorbent to adsorb nucleic acids derived from the whole blood sample onto the first nucleic acid adsorbent, and then separating and removing the first nucleic acid adsorbent to recover liquid components.

Abstract translation: 提供了一种回收短链核酸的方法，其包括：使醇浓度为0至5体积％的混合物的步骤（a），所述混合物通过将全血样品与含有一种 或更多选自离液剂和表面活性剂的成分与第一核酸吸附剂接触以将从全血样品衍生的核酸吸附到第一核酸吸附剂上，然后分离和除去第一核酸 吸附剂回收液体成分。

公开(公告)号：US20180230452A1

公开(公告)日：2018-08-16

申请号：US15948124

申请日：2018-04-09

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Hiroki NAGASE ,  Kei TSUKAMOTO ,  Shiro KITANO

IPC: C12N15/10 ,  C08G73/06 ,  C08G69/10 ,  G01N1/40

CPC classification number: C12N15/1006 ,  C07H1/06 ,  C07H21/00 ,  C08G69/10 ,  C08G73/0611 ,  C08G73/0616 ,  C12N15/09 ,  G01N1/405 ,  C12Q2525/191 ,  C12Q2537/159

Abstract: A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.

公开(公告)号：US20230323310A1

公开(公告)日：2023-10-12

申请号：US18043507

申请日：2021-09-03

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Tomoko KUNITOMI ,  Shiro KITANO ,  Kei TSUKAMOTO ,  Michiya MATSUSAKI

IPC: C12N5/071 ,  C12N5/077

CPC classification number: C12N5/069 ,  C12N5/0656 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2501/91

Abstract: The present invention relates to a method for producing a three-dimensional tissue body, including: a step of obtaining a mixture by mixing a cationic substance and a fragmented extracellular matrix component with cells; and a step of culturing the cells after the step of obtaining the mixture.