公开(公告)号：US09217727B2

公开(公告)日：2015-12-22

申请号：US13787341

申请日：2013-03-06

申请人： The Trustees of Columbia University in the City of New York

发明人： Jacob Rosenstein ,  Kenneth L. Shepard

IPC分类号： G01N27/447 ,  B82Y5/00 ,  C12Q1/68 ,  B82Y15/00 ,  G01N33/68 ,  G01N33/487

CPC分类号： G01N27/44704 ,  B82Y5/00 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/44713 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/68 ,  Y10S977/852

摘要： A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.

摘要翻译： 提供一种使用集成电路检测单分子的系统和方法，该集成电路包括至少一个具有位于第一和第二储存器之间的纳米孔的膜和在其表面上形成有电极的低噪声前置放大器。 该方法包括使靶分子通过纳米孔，并测量通过纳米孔的电流，以检测生物分子实体的存在（如果有的话）。

公开(公告)号：US20150214384A1

公开(公告)日：2015-07-30

申请号：US14681753

申请日：2015-04-08

申请人： The Trustees of Columbia University in the City of New York

发明人： KENNETH L. SHEPARD ,  Jacob Rosenstein ,  Ryan Michael Field ,  Dan Fleischer

IPC分类号： H01L29/808 ,  H01L21/762 ,  H01L29/423 ,  H01L27/098 ,  H01L21/8232 ,  H01L29/66 ,  H01L29/06

CPC分类号： H01L29/808 ,  H01L21/762 ,  H01L21/8232 ,  H01L27/098 ,  H01L29/0649 ,  H01L29/0696 ,  H01L29/42316 ,  H01L29/66893

摘要： A complementary metal oxide semiconductor (CMOS)-integrated junction field effect transistor (JFET) has reduced scale and reduced noise. An exemplary JFET has a substrate layer of one dopant type with a gate layer of that dopant type disposed on the substrate, a depletion channel of a second dopant type disposed on the first gate layer, and a second gate layer of the first dopant type disposed on the depletion channel and proximate a surface of the transistor. The second gate layer can separate the depletion channel from the surface, and the depletion channel separates the first gate layer from the second gate layer.

摘要翻译： 互补金属氧化物半导体（CMOS） - 集成结型场效应晶体管（JFET）具有减小的尺寸和降低的噪声。 示例性JFET具有一种掺杂剂型的衬底层，其中设置在衬底上的该掺杂剂类型的栅极层，设置在第一栅极层上的第二掺杂剂类型的耗尽沟道和设置在第一掺杂剂类型的第二栅极层 在耗尽通道上并且靠近晶体管的表面。 第二栅极层可以将耗尽沟道与表面分开，耗尽沟道将第一栅极层与第二栅极层分开。

公开(公告)号：US20150090588A1

公开(公告)日：2015-04-02

申请号：US14509594

申请日：2014-10-08

申请人： The Trustees of Columbia University in the City of New York

发明人： Kenneth L. Shepard ,  Jacob Rosenstein ,  Siddharth Ramakrishnan ,  Jared Roseman

IPC分类号： G01N27/414 ,  H05K3/30

CPC分类号： G01N27/4148 ,  G01N33/48728 ,  G01N33/5438 ,  H05K3/303 ,  Y10T29/49147

摘要： Method for interfacing an integrated circuit with a biological ion channel, the integrated circuit being at least partially disposed within an electrolytic solution and including an amplifier and one or more electrodes on a surface thereof, includes forming one or more microwells proximate the one or more electrodes, applying a lipid membrane over the integrated circuit proximate the microwells, and placing a further electrode in the electrolytic solution proximate the lipid membrane on a side opposite the integrated circuit. A biological ion channel interface is also provided.

摘要翻译： 用于将集成电路与生物离子通道接合的方法，所述集成电路至少部分地设置在电解溶液中并且包括放大器和其表面上的一个或多个电极，包括在所述一个或多个电极附近形成一个或多个微孔 在靠近微孔的集成电路上施加脂质膜，并将另外的电极放置在靠近集成电路相对侧的脂质膜的电解液中。 还提供生物离子通道界面。

公开(公告)号：US11624727B2

公开(公告)日：2023-04-11

申请号：US14837514

申请日：2015-08-27

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Jacob Rosenstein ,  Kenneth L. Shepard

IPC分类号： G01N27/447 ,  B82Y5/00 ,  C12Q1/6869 ,  B82Y15/00 ,  G01N33/68 ,  G01N33/487

摘要： A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.

公开(公告)号：US09741870B2

公开(公告)日：2017-08-22

申请号：US14681753

申请日：2015-04-08

申请人： The Trustees of Columbia University in the City of New York

发明人： Kenneth L. Shepard ,  Jacob Rosenstein ,  Ryan Michael Field ,  Dan Fleischer

IPC分类号： H01L29/808 ,  H01L29/06 ,  H01L21/762 ,  H01L21/8232 ,  H01L27/098 ,  H01L29/423 ,  H01L29/66

CPC分类号： H01L29/808 ,  H01L21/762 ,  H01L21/8232 ,  H01L27/098 ,  H01L29/0649 ,  H01L29/0696 ,  H01L29/42316 ,  H01L29/66893

摘要： A complementary metal oxide semiconductor (CMOS)-integrated junction field effect transistor (JFET) has reduced scale and reduced noise. An exemplary JFET has a substrate layer of one dopant type with a gate layer of that dopant type disposed on the substrate, a depletion channel of a second dopant type disposed on the first gate layer, and a second gate layer of the first dopant type disposed on the depletion channel and proximate a surface of the transistor. The second gate layer can separate the depletion channel from the surface, and the depletion channel separates the first gate layer from the second gate layer.

公开(公告)号：US20140048416A1

公开(公告)日：2014-02-20

申请号：US13942242

申请日：2013-07-15

申请人： The Trustees of Columbia University in the City of New York

发明人： Jacob Rosenstein ,  Kenneth L. Shepard

IPC分类号： G01N27/447

CPC分类号： G01N27/44704 ,  B82Y5/00 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/44713 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/68 ,  Y10S977/852

摘要： A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.

摘要翻译： 提供一种使用集成电路检测单分子的系统和方法，该集成电路包括至少一个具有位于第一和第二储存器之间的纳米孔的膜和在其表面上形成有电极的低噪声前置放大器。 该方法包括使靶分子通过纳米孔，并测量通过纳米孔的电流，以检测生物分子实体的存在（如果有的话）。

公开(公告)号：US20150369776A1

公开(公告)日：2015-12-24

申请号：US14837514

申请日：2015-08-27

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Jacob Rosenstein ,  Kenneth L. Shepard

IPC分类号： G01N27/447

CPC分类号： G01N27/44704 ,  B82Y5/00 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/44713 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/68 ,  Y10S977/852

摘要： A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.

摘要翻译： 提供一种使用集成电路检测单分子的系统和方法，该集成电路包括至少一个具有位于第一和第二储存器之间的纳米孔的膜和在其表面上形成有电极的低噪声前置放大器。 该方法包括使靶分子通过纳米孔，并测量通过纳米孔的电流，以检测生物分子实体的存在（如果有的话）。

公开(公告)号：US20130180867A1

公开(公告)日：2013-07-18

申请号：US13787341

申请日：2013-03-06

申请人： The Trustees of Columbia University in the City of New York

发明人： Jacob Rosenstein ,  Kenneth L. Shepard

IPC分类号： C12Q1/00 ,  G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44704 ,  B82Y5/00 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/44713 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/68 ,  Y10S977/852

摘要： A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.