公开(公告)号：US20200377898A1

公开(公告)日：2020-12-03

申请号：US16070648

申请日：2017-02-02

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： Lianghui JI ,  Yanbin LIU ,  Chong Mei John KOH ,  Sihui Amy YAP

IPC: C12N15/81 ,  C12P1/02

Abstract: The present invention relates to the field of molecular biology and more particularly to promoters useful for metabolic engineering in yeast or fungi for the production of biobased chemicals with broad applications. Intron-containing promoters with strong activity during oil-accumulation stages are particularly useful for genetic engineering in yeast and fungi, particularly Rhodosporidium or Rhodotorula genera. Such promoter are capable of driving strong expression of RNA or proteins in species of the Rhodosporidium or Rhodotorula genera.

公开(公告)号：US20170362285A1

公开(公告)日：2017-12-21

申请号：US15535466

申请日：2015-12-14

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： Yanbin LIU ,  Chong Mei KOH ,  Lianghui JI

IPC: C07K14/37 ,  C12N9/02 ,  C12N15/80 ,  C12N15/113 ,  C12P23/00 ,  C12P21/02

CPC classification number: C07K14/37 ,  C12N9/0008 ,  C12N15/113 ,  C12N15/80 ,  C12P21/02 ,  C12P23/00

Abstract: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of carotenoids in fungal hosts selected from Rhodospordium and Rhodotorula genera.

公开(公告)号：US20180073034A1

公开(公告)日：2018-03-15

申请号：US15563176

申请日：2016-03-31

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： Lianghui JI ,  Yanbin LIU ,  Chong Mei John KOH

IPC: C12N15/81 ,  C12N1/16

CPC classification number: C12N15/815 ,  C12N1/16 ,  C12N15/80 ,  C12N15/81 ,  C12N2830/36 ,  C12N2840/002

Abstract: The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.

公开(公告)号：US20170198315A1

公开(公告)日：2017-07-13

申请号：US15326077

申请日：2015-08-21

Applicant: Temasek Life Sciences Laboratory Limited

Inventor： Yanbin LIU ,  Chong Mei KOH ,  Lianghui JI

IPC: C12P7/64 ,  C12N15/52 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00 ,  C12N9/04 ,  C12N9/02 ,  C12N9/90

CPC classification number: C12P7/6427 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/52 ,  C12N15/81 ,  C12P7/6463 ,  C12Y101/0104 ,  C12Y102/01003 ,  C12Y102/01012 ,  C12Y114/19 ,  C12Y114/19001 ,  C12Y114/19003 ,  C12Y114/19006 ,  C12Y203/0102 ,  C12Y203/03008 ,  C12Y401/01001 ,  C12Y402/01011 ,  C12Y502/01008 ,  C12Y602/01003 ,  C12Y604/01002

Abstract: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of polyunsaturated fatty acids (PUFA) in fungal hosts selected from Rhodosporidium and Rhodotorula genera. The present invention further relates to a modified fungal host cell having reduced native aldehyde dehydrogenase (ALD 1) enzyme activity, and methods for producing omega-3 and omega-6 fatty acids and triacylglycerides, by growing said fungal host cell under suitable conditions.