Transgenic seeds expressing amylopullulanase and uses therefor
    1.
    发明授权
    Transgenic seeds expressing amylopullulanase and uses therefor 有权
    转基因种子表达amylopullululanase和用途

    公开(公告)号:US06737563B2

    公开(公告)日:2004-05-18

    申请号:US10050763

    申请日:2002-01-16

    CPC classification number: C12N15/8251 C12N15/8245 C12P19/16

    Abstract: The invention provides DNA constructs and genetically engineered seeds for the expression of amylopullulanase in plant seeds such as rice seeds. Related methods are also provided for the production of sugars, modified starches, and high protein products, and use of the glutelin promoter in the methods.

    Abstract translation: 本发明提供DNA构建体和用于在植物种子例如水稻种子中表达淀粉样蛋白酶的基因工程种子。 还提供了相关方法用于生产糖,改性淀粉和高蛋白产物,以及在该方法中使用谷蛋白启动子。

    Aarobacterium-mediated method for transforming rice
    5.
    发明授权
    Aarobacterium-mediated method for transforming rice 失效
    农杆菌介导的水稻转化方法

    公开(公告)号:US06215051B1

    公开(公告)日:2001-04-10

    申请号:US09072435

    申请日:1998-05-04

    CPC classification number: C12N15/8222 C12N15/8234 C12N15/8238

    Abstract: The present invention is directed to a method for the production of a transgenic plant of rice crop comprising the steps of infecting an immature embryo of rice crop with the genus Agrobacterium for transformation; co-culturing the infected embryo with a dicot suspension culture during the step of transformation; allowing the transformed embryo to grow into a callus in a selective medium comprising a sufficient amount of a plant growth hormone for the growth of rice crop; and allowing the cultured callus to regenerate root and shoot in a regeneration medium comprising a pre-determined amount of nutrients for the growth of rice crop. The invention is further directed to a transformed rice plant made by methods of this invention.

    Abstract translation: 本发明涉及一种用于生产水稻作物转基因植物的方法,包括用农杆菌感染水稻作物的未成熟胚进行转化的步骤; 在转化步骤期间用双子叶悬浮培养物共同培养感染的胚胎; 使得转化的胚胎在包含足够量的用于水稻作物生长的植物生长激素的选择培养基中生长成愈伤组织; 并且允许培养的愈伤组织在包含用于水稻作物生长的预定量的营养物质的再生培养基中再生根和芽。 本发明进一步涉及通过本发明的方法制备的转化水稻植物。

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