Extracellular vesicle isolation by nanomembranes

    公开(公告)号:US11119013B2

    公开(公告)日:2021-09-14

    申请号:US16600322

    申请日:2019-10-11

    申请人: SiMPore, Inc.

    摘要: Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereb forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

    Extracellular vesicle isolation by nanomembranes

    公开(公告)号:US11635357B2

    公开(公告)日:2023-04-25

    申请号:US17382505

    申请日:2021-07-22

    申请人: SiMPore Inc.

    摘要: Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.