公开(公告)号：US11119013B2

公开(公告)日：2021-09-14

申请号：US16600322

申请日：2019-10-11

申请人： SiMPore, Inc.

发明人： Jared A. Carter ,  Akash S. Patel ,  Cassandra R. Walinski ,  James A. Roussie

IPC分类号： B01D61/02 ,  B01L3/00 ,  G01N1/40 ,  B01D71/02 ,  G01N1/28

摘要： Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereb forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

公开(公告)号：US11635357B2

公开(公告)日：2023-04-25

申请号：US17382505

申请日：2021-07-22

申请人： SiMPore Inc.

发明人： Jared A. Carter ,  Akash S. Patel ,  Cassandra R. Walinski ,  James A. Roussie

IPC分类号： B01D61/02 ,  B01D61/14 ,  B01D65/08 ,  G01N1/28 ,  G01N1/40 ,  B01D71/02 ,  B01L3/00

摘要： Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

公开(公告)号：US20210215584A1

公开(公告)日：2021-07-15

申请号：US17268330

申请日：2019-08-16

申请人： SIMPORE INC.

发明人： Jared A. Carter ,  James A. Roussie

IPC分类号： G01N1/40 ,  G01N33/50 ,  B01D61/18 ,  B01D61/14 ,  B01D69/02 ,  B01D71/02

摘要： Provided are methods, devices, and kits for the isolation and enumeration of one or more components of interest within a liquid sample using microslit filter membranes. This disclosure relates to the enumeration of components within a sample of interest, and more particularly, the capture of such components by efficient isolation using microslit filters with high permeation capacity and precision molecular cut-off characteristics.